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- W2097632166 abstract "Glycosylphosphatidylinositol (GPI) anchoring of proteins to the cell surface is important for various biological processes, but GPI-anchored proteins are difficult to study. An effective strategy was developed for the metabolic engineering of cell-surface GPIs and GPI-anchored proteins by using inositol derivatives carrying an azido group. The azide-labeled GPIs and GPI-anchored proteins were then tagged with biotin on live cells through a click reaction, which allows further elaboration with streptavidin-conjugated dyes or other molecules. The strategy can be used to label GPI-anchored proteins with various tags for biological studies." @default.
- W2097632166 created "2016-06-24" @default.
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- W2097632166 date "2015-06-23" @default.
- W2097632166 modified "2023-09-27" @default.
- W2097632166 title "Labeling Cell Surface GPIs and GPI-Anchored Proteins through Metabolic Engineering with Artificial Inositol Derivatives" @default.
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- W2097632166 doi "https://doi.org/10.1002/anie.201503814" @default.
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