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- W2097634931 abstract "Some like it hot: We previously described a laboratory-evolved variant of the cytochrome P450 BM-3 heme domain which functions as a peroxide-driven hydroxylase (peroxygenase, see picture). This biocatalyst does not require additional proteins or NADPH to drive hydroxylation and is amenable to further improvements through protein engineering. Here we describe a thermostable variant whose half-life at 57.5 °C is 50 times that of the F87A variant and 250 times that of the wildtype holoenzyme." @default.
- W2097634931 created "2016-06-24" @default.
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- W2097634931 date "2003-09-04" @default.
- W2097634931 modified "2023-10-17" @default.
- W2097634931 title "Thermostabilization of a Cytochrome P450 Peroxygenase" @default.
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- W2097634931 doi "https://doi.org/10.1002/cbic.200300660" @default.
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