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• W2097718887 endingPage "2291" @default.
• W2097718887 startingPage "2281" @default.
• W2097718887 abstract "In mammalian cells, the core factors involved in the damage recognition and incision steps of DNA nucleotide excision repair are XPA, TFIIH complex, XPC-HR23B, replication protein A (RPA), XPG, and ERCC1-XPF. Many interactions between these components have been detected, using different physical methods, in human cells and for the homologous factors in Saccharomyces cerevisiae. Several human nucleotide excision repair (NER) complexes, including a high-molecular-mass repairosome complex, have been proposed. However, there have been no measurements of activity of any mammalian NER protein complex isolated under native conditions. In order to assess relative strengths of interactions between NER factors, we captured TFIIH from cell extracts with an anti-cdk7 antibody, retaining TFIIH in active form attached to magnetic beads. Coimmunoprecipitation of other NER proteins was then monitored functionally in a reconstituted repair system with purified proteins. We found that all detectable TFIIH in gently prepared human cell extracts was present in the intact nine-subunit form. There was no evidence for a repair complex that contained all of the NER components. At low ionic strength TFIIH could associate with functional amounts of each NER factor except RPA. At physiological ionic strength, TFIIH associated with significant amounts of XPC-HR23B and XPG but not other repair factors. The strongest interaction was between TFIIH and XPC-HR23B, indicating a coupled role of these proteins in early steps of repair. A panel of antibodies was used to estimate that there are on the order of 10(5) molecules of each core NER factor per HeLa cell." @default.
• W2097718887 created "2016-06-24" @default.
• W2097718887 creator A5065003651 @default.
• W2097718887 creator A5067286626 @default.
• W2097718887 creator A5081633286 @default.
• W2097718887 date "2001-04-01" @default.
• W2097718887 modified "2023-10-17" @default.
• W2097718887 title "Strong Functional Interactions of TFIIH with XPC and XPG in Human DNA Nucleotide Excision Repair, without a Preassembled Repairosome" @default.
• W2097718887 cites W1514802418 @default.
• W2097718887 cites W1533908104 @default.
• W2097718887 cites W1556435247 @default.
• W2097718887 cites W1558614105 @default.
• W2097718887 cites W1809038905 @default.
• W2097718887 cites W1886853229 @default.
• W2097718887 cites W1961821398 @default.
• W2097718887 cites W1964198669 @default.
• W2097718887 cites W1967288647 @default.
• W2097718887 cites W1975494947 @default.
• W2097718887 cites W1975853177 @default.
• W2097718887 cites W1982307936 @default.
• W2097718887 cites W1982918697 @default.
• W2097718887 cites W1987430471 @default.
• W2097718887 cites W1992431849 @default.
• W2097718887 cites W1996172944 @default.
• W2097718887 cites W1999602003 @default.
• W2097718887 cites W2000814287 @default.
• W2097718887 cites W2000983378 @default.
• W2097718887 cites W2001569092 @default.
• W2097718887 cites W2004431479 @default.
• W2097718887 cites W2007998684 @default.
• W2097718887 cites W2010992218 @default.
• W2097718887 cites W2013230822 @default.
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• W2097718887 cites W2022561772 @default.
• W2097718887 cites W2025571437 @default.
• W2097718887 cites W2028919668 @default.
• W2097718887 cites W2033180282 @default.
• W2097718887 cites W2036103078 @default.
• W2097718887 cites W2038267351 @default.
• W2097718887 cites W2039068784 @default.
• W2097718887 cites W2039740270 @default.
• W2097718887 cites W2041514249 @default.
• W2097718887 cites W2043587249 @default.
• W2097718887 cites W2044000184 @default.
• W2097718887 cites W2048725347 @default.
• W2097718887 cites W2049812251 @default.
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• W2097718887 cites W2059299769 @default.
• W2097718887 cites W2062582450 @default.
• W2097718887 cites W2063260609 @default.
• W2097718887 cites W2064235474 @default.
• W2097718887 cites W2070184329 @default.
• W2097718887 cites W2070821144 @default.
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• W2097718887 cites W2150072036 @default.
• W2097718887 cites W2163864783 @default.
• W2097718887 cites W2166593574 @default.
• W2097718887 cites W2171578468 @default.
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• W2097718887 doi "https://doi.org/10.1128/mcb.21.7.2281-2291.2001" @default.
• W2097718887 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/86862" @default.
• W2097718887 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11259578" @default.
• W2097718887 hasPublicationYear "2001" @default.
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