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• W2097764474 abstract "ABSTRACT LuxR is the transcriptional activator for quorum-sensing control of luminescence in Vibrio fischeri . A series of alanine-scanning mutants spanning a predicted helix-turn-helix region in the DNA binding domain of LuxR was constructed, and the activity of each of the LuxR mutant proteins in recombinant Escherichia coli was investigated. The region covered by the mutagenesis spanned residues 190 to 224. About half of the alanine-scanning mutants showed activities similar to that of the wild-type LuxR: at least two were positive-control mutants, four appeared to be defective in DNA binding, and several others were characterized as DNA binding affinity mutants. This analysis, taken together with information about other bacterial transcription factors, provides insights into amino acid residues in LuxR that are involved in DNA binding and transcriptional activation." @default.
• W2097764474 created "2016-06-24" @default.
• W2097764474 creator A5022848501 @default.
• W2097764474 creator A5084243657 @default.
• W2097764474 date "2001-01-01" @default.
• W2097764474 modified "2023-09-30" @default.
• W2097764474 title "Quorum Sensing in <i>Vibrio fischeri</i> : Analysis of the LuxR DNA Binding Region by Alanine-Scanning Mutagenesis" @default.
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• W2097764474 doi "https://doi.org/10.1128/jb.183.1.382-386.2001" @default.
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