FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2098049179> ?p ?o ?g. }

• W2098049179 endingPage "405" @default.
• W2098049179 startingPage "398" @default.
• W2098049179 abstract "The pregnane X receptor (PXR, NR1I2) plays a pivotal role in the disposition and detoxification of numerous foreign and endogenous chemicals by increasing transcription of numerous target genes, including phase I and II drug-metabolizing enzymes and transporters. In the present study, yeast two-hybrid screening identified an E3 ubiquitin ligase, RBCK1 (Ring-B-box-coiled-coil protein interacting with protein kinase C-1), as a human pregnane X receptor (hPXR)–interacting protein. Coimmunoprecipitation studies confirmed the interaction between RBCK1 and hPXR when both were ectopically expressed in AD-293 cells. Domain mapping studies showed that the interaction between RBCK1 and hPXR involves all RBCK1 domains. We further demonstrate that RBCK1 ubiquitinates hPXR, and this may target hPXR for degradation by the ubiquitin-proteasome pathway. Simultaneous ectopic overexpression of RBCK1 and PXR decreased PXR levels in AD-293 cells, and this decrease was inhibited by the proteasomal inhibitor MG-132 (carbobenzoxy-Leu-Leu-leucinal). Furthermore, overexpression of RBCK1 decreased endogenous levels of PXR in HepG2 cells. Of importance, ectopic overexpression and silencing of endogenous RBCK1 in primary human hepatocytes resulted in a decrease and increase, respectively, in endogenous PXR protein levels and in the induction of PXR target genes by rifampicin. These results suggest that RBCK1 is important for the ubiquitination of PXR and may play a role in its proteasomal degradation." @default.
• W2098049179 created "2016-06-24" @default.
• W2098049179 creator A5040571618 @default.
• W2098049179 creator A5062829421 @default.
• W2098049179 creator A5065650387 @default.
• W2098049179 creator A5085504945 @default.
• W2098049179 date "2012-11-15" @default.
• W2098049179 modified "2023-10-15" @default.
• W2098049179 title "RBCK1, an E3 Ubiquitin Ligase, Interacts with and Ubiquinates the Human Pregnane X Receptor" @default.
• W2098049179 cites W1835084434 @default.
• W2098049179 cites W1887243203 @default.
• W2098049179 cites W1968535758 @default.
• W2098049179 cites W1971042451 @default.
• W2098049179 cites W1978091865 @default.
• W2098049179 cites W1997901770 @default.
• W2098049179 cites W1998851985 @default.
• W2098049179 cites W2003996311 @default.
• W2098049179 cites W2005322337 @default.
• W2098049179 cites W2008458519 @default.
• W2098049179 cites W2013599007 @default.
• W2098049179 cites W2016194944 @default.
• W2098049179 cites W2019349841 @default.
• W2098049179 cites W2021444563 @default.
• W2098049179 cites W2025748949 @default.
• W2098049179 cites W2026383483 @default.
• W2098049179 cites W2035000881 @default.
• W2098049179 cites W2040849706 @default.
• W2098049179 cites W2041417196 @default.
• W2098049179 cites W2042763169 @default.
• W2098049179 cites W2047195343 @default.
• W2098049179 cites W2050836489 @default.
• W2098049179 cites W2053186448 @default.
• W2098049179 cites W2084462922 @default.
• W2098049179 cites W2096732383 @default.
• W2098049179 cites W2098720060 @default.
• W2098049179 cites W2128374908 @default.
• W2098049179 cites W2130448025 @default.
• W2098049179 cites W2132479491 @default.
• W2098049179 cites W2134757153 @default.
• W2098049179 cites W2140559258 @default.
• W2098049179 cites W2142783300 @default.
• W2098049179 cites W2148059499 @default.
• W2098049179 cites W2150123812 @default.
• W2098049179 cites W2155616928 @default.
• W2098049179 cites W2157166953 @default.
• W2098049179 cites W2157906581 @default.
• W2098049179 cites W2161179748 @default.
• W2098049179 cites W2167076974 @default.
• W2098049179 cites W4243182447 @default.
• W2098049179 doi "https://doi.org/10.1124/dmd.112.048728" @default.
• W2098049179 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3558864" @default.
• W2098049179 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23160820" @default.
• W2098049179 hasPublicationYear "2012" @default.
• W2098049179 type Work @default.
• W2098049179 sameAs 2098049179 @default.
• W2098049179 citedByCount "28" @default.
• W2098049179 countsByYear W20980491792013 @default.
• W2098049179 countsByYear W20980491792014 @default.
• W2098049179 countsByYear W20980491792015 @default.
• W2098049179 countsByYear W20980491792016 @default.
• W2098049179 countsByYear W20980491792017 @default.
• W2098049179 countsByYear W20980491792018 @default.
• W2098049179 countsByYear W20980491792020 @default.
• W2098049179 countsByYear W20980491792021 @default.
• W2098049179 countsByYear W20980491792022 @default.
• W2098049179 countsByYear W20980491792023 @default.
• W2098049179 crossrefType "journal-article" @default.
• W2098049179 hasAuthorship W2098049179A5040571618 @default.
• W2098049179 hasAuthorship W2098049179A5062829421 @default.
• W2098049179 hasAuthorship W2098049179A5065650387 @default.
• W2098049179 hasAuthorship W2098049179A5085504945 @default.
• W2098049179 hasBestOaLocation W20980491792 @default.
• W2098049179 hasConcept C104317684 @default.
• W2098049179 hasConcept C131312102 @default.
• W2098049179 hasConcept C132040763 @default.
• W2098049179 hasConcept C134459356 @default.
• W2098049179 hasConcept C153911025 @default.
• W2098049179 hasConcept C16613235 @default.
• W2098049179 hasConcept C185592680 @default.
• W2098049179 hasConcept C25602115 @default.
• W2098049179 hasConcept C27740335 @default.
• W2098049179 hasConcept C55493867 @default.
• W2098049179 hasConcept C63932345 @default.
• W2098049179 hasConcept C86339819 @default.
• W2098049179 hasConcept C86803240 @default.
• W2098049179 hasConcept C95444343 @default.
• W2098049179 hasConcept C97037327 @default.
• W2098049179 hasConceptScore W2098049179C104317684 @default.
• W2098049179 hasConceptScore W2098049179C131312102 @default.
• W2098049179 hasConceptScore W2098049179C132040763 @default.
• W2098049179 hasConceptScore W2098049179C134459356 @default.
• W2098049179 hasConceptScore W2098049179C153911025 @default.
• W2098049179 hasConceptScore W2098049179C16613235 @default.
• W2098049179 hasConceptScore W2098049179C185592680 @default.
• W2098049179 hasConceptScore W2098049179C25602115 @default.
• W2098049179 hasConceptScore W2098049179C27740335 @default.
• W2098049179 hasConceptScore W2098049179C55493867 @default.
• W2098049179 hasConceptScore W2098049179C63932345 @default.

• next