Matches in SemOpenAlex for { <https://semopenalex.org/work/W2098056895> ?p ?o ?g. }
- W2098056895 endingPage "3648" @default.
- W2098056895 startingPage "3636" @default.
- W2098056895 abstract "Contact inhibition ubiquitously exists in non-transformed cells that are in contact with neighboring cells. This phenomenon explains the poor regenerative capacity of in vivo human corneal endothelial cells during aging, injury and surgery. This study demonstrated that the conventional approach of expanding human corneal endothelial cells by disrupting contact inhibition with EDTA followed by bFGF activated canonical Wnt signaling and lost the normal phenotype to endothelial-mesenchymal transition, especially if TGFβ1 was added. By contrast, siRNA against p120 catenin (CTNND1) also uniquely promoted proliferation of the endothelial cells by activating trafficking of p120 catenin to the nucleus, thus relieving repression by nuclear Kaiso. This nuclear p120-catenin-Kaiso signaling is associated with activation of RhoA-ROCK signaling, destabilization of microtubules and inhibition of Hippo signaling, but not with activation of Wnt-β-catenin signaling. Consequently, proliferating human corneal endothelial cells maintained a hexagonal shape, with junctional expression of N-cadherin, ZO-1 and Na(+)/K(+)-ATPase. Further expansion of human corneal endothelial monolayers with a normal phenotype and a higher density was possible by prolonging treatment with p120 catenin siRNA followed by its withdrawal. This new strategy of perturbing contact inhibition by selective activation of p120-catenin-Kaiso signaling without disrupting adherent junction could be used to engineer surgical grafts containing normal human corneal endothelial cells to meet a global corneal shortage and for endothelial keratoplasties." @default.
- W2098056895 created "2016-06-24" @default.
- W2098056895 creator A5017290614 @default.
- W2098056895 creator A5031915479 @default.
- W2098056895 creator A5032718030 @default.
- W2098056895 creator A5044834301 @default.
- W2098056895 date "2012-08-01" @default.
- W2098056895 modified "2023-10-11" @default.
- W2098056895 title "Nuclear p120 catenin unlocks mitotic block of contact-inhibited human corneal endothelial monolayers without disrupting adherent junctions" @default.
- W2098056895 cites W1583843945 @default.
- W2098056895 cites W1744742580 @default.
- W2098056895 cites W1934202741 @default.
- W2098056895 cites W1965950604 @default.
- W2098056895 cites W1969016804 @default.
- W2098056895 cites W1974673111 @default.
- W2098056895 cites W1976561610 @default.
- W2098056895 cites W1977125048 @default.
- W2098056895 cites W1977286350 @default.
- W2098056895 cites W1983057716 @default.
- W2098056895 cites W1991976042 @default.
- W2098056895 cites W2002253933 @default.
- W2098056895 cites W2002278797 @default.
- W2098056895 cites W2007916618 @default.
- W2098056895 cites W2008763684 @default.
- W2098056895 cites W2009775095 @default.
- W2098056895 cites W2013067580 @default.
- W2098056895 cites W2016056829 @default.
- W2098056895 cites W2040210675 @default.
- W2098056895 cites W2041614750 @default.
- W2098056895 cites W2043656350 @default.
- W2098056895 cites W2046825731 @default.
- W2098056895 cites W2048616064 @default.
- W2098056895 cites W2051696368 @default.
- W2098056895 cites W2057257265 @default.
- W2098056895 cites W2058497055 @default.
- W2098056895 cites W2059204760 @default.
- W2098056895 cites W2064381695 @default.
- W2098056895 cites W2065131699 @default.
- W2098056895 cites W2065723711 @default.
- W2098056895 cites W2067977003 @default.
- W2098056895 cites W2071765960 @default.
- W2098056895 cites W2078063257 @default.
- W2098056895 cites W2079110639 @default.
- W2098056895 cites W2085179400 @default.
- W2098056895 cites W2093590754 @default.
- W2098056895 cites W2095404261 @default.
- W2098056895 cites W2105241867 @default.
- W2098056895 cites W2108251346 @default.
- W2098056895 cites W2114718608 @default.
- W2098056895 cites W2124059822 @default.
- W2098056895 cites W2129140621 @default.
- W2098056895 cites W2131679528 @default.
- W2098056895 cites W2135726634 @default.
- W2098056895 cites W2136991739 @default.
- W2098056895 cites W2141759421 @default.
- W2098056895 cites W2146473598 @default.
- W2098056895 cites W2148294117 @default.
- W2098056895 cites W2150163671 @default.
- W2098056895 cites W2151269972 @default.
- W2098056895 cites W2157845902 @default.
- W2098056895 cites W2161918376 @default.
- W2098056895 cites W2169745186 @default.
- W2098056895 doi "https://doi.org/10.1242/jcs.103267" @default.
- W2098056895 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3445326" @default.
- W2098056895 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22505615" @default.
- W2098056895 hasPublicationYear "2012" @default.
- W2098056895 type Work @default.
- W2098056895 sameAs 2098056895 @default.
- W2098056895 citedByCount "92" @default.
- W2098056895 countsByYear W20980568952012 @default.
- W2098056895 countsByYear W20980568952013 @default.
- W2098056895 countsByYear W20980568952014 @default.
- W2098056895 countsByYear W20980568952015 @default.
- W2098056895 countsByYear W20980568952016 @default.
- W2098056895 countsByYear W20980568952017 @default.
- W2098056895 countsByYear W20980568952018 @default.
- W2098056895 countsByYear W20980568952019 @default.
- W2098056895 countsByYear W20980568952020 @default.
- W2098056895 countsByYear W20980568952021 @default.
- W2098056895 countsByYear W20980568952022 @default.
- W2098056895 countsByYear W20980568952023 @default.
- W2098056895 crossrefType "journal-article" @default.
- W2098056895 hasAuthorship W2098056895A5017290614 @default.
- W2098056895 hasAuthorship W2098056895A5031915479 @default.
- W2098056895 hasAuthorship W2098056895A5032718030 @default.
- W2098056895 hasAuthorship W2098056895A5044834301 @default.
- W2098056895 hasBestOaLocation W20980568951 @default.
- W2098056895 hasConcept C123012128 @default.
- W2098056895 hasConcept C137620995 @default.
- W2098056895 hasConcept C202751555 @default.
- W2098056895 hasConcept C2777093181 @default.
- W2098056895 hasConcept C30145163 @default.
- W2098056895 hasConcept C55493867 @default.
- W2098056895 hasConcept C62112901 @default.
- W2098056895 hasConcept C62478195 @default.
- W2098056895 hasConcept C64979461 @default.
- W2098056895 hasConcept C86803240 @default.
- W2098056895 hasConcept C95444343 @default.