Matches in SemOpenAlex for { <https://semopenalex.org/work/W2098081309> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2098081309 endingPage "tw283" @default.
- W2098081309 startingPage "tw283" @default.
- W2098081309 abstract "Scratching monolayers of tissue culture cells and observing the changes that occur in those cells closest to the scratch have provided important insights into how cells polarize and coordinate their movement to repair the damage. The area of the cell closest to the scratch (essentially the new front of the cell) becomes the site of actin-rich protrusions, whereas rearrangements of the microtubule cytoskeleton push the centrosome and Golgi toward the front of the cell and push the nucleus toward the back. This polarization of the cell is dependent on the activity of the Rho family guanosine triphosphatase, Cdc42, which also leads to the assembly of a complex between the scaffold protein Par6 and atypical protein kinase C (aPKC). This complex is necessary for the accumulation of adenomatous polyposis coli (APC), a tumor suppressor protein involved in Wnt signaling. Inhibition of the activity of glycogen synthase kinase (GSK)-3β by PKC phosphorylation is presumed to allow the accumulation of APC at the leading edge. However, experiments by Schlessinger et al. suggest that the interaction between GSK-3β and aPKC is not the relevant one. They generated double knockin mice (GSK-3SA) in which the relevant Ser residues of both GSK-3α and GSK-3β were mutated to Ala residues, thus preventing their inhibition by phosphorylation. Unexpectedly, movement of the Golgi and centrosome in response to monolayer scratching of fibroblasts from GSK-3SA mice was normal. Dishevelled (Dvl) is another component in the Wnt pathway, and Wnt stimulates the binding of Dvl to the scaffold protein axin, which results in the release of GSK-3β from a complex containing axin, APC, and β-catenin. Knockdown of Dvl or axin with siRNA in rat embryo fibroblasts inhibited reorientation of both the centrosome and Golgi, as did treatment of scratched monolayers with a Wnt-binding protein, demonstrating the importance of Wnt signaling in cell polarization. Inhibition of Wnt5a, but not Wnt1 or Wnt3a, by siRNA blocked reorientation of the fibroblasts in scratched monolayers and also inhibited the accumulation of APC to microtubules at the leading edge, as demonstrated by confocal microscopy. Western blotting demonstrated that scratching of the monolayer resulted in the phosphorylation of Dvl proteins that was dependent on Wnt signaling. Finally, coimmunoprecipitation studies showed that aPKC and Dvl2 formed a complex after scratching of monolayers, which was dependent on both Wnt and Cdc42, as demonstrated by the use of Wnt-specific siRNA and an inhibitor of Cdc42. Thus, Cdc42-dependent Wnt signaling appears to play a role in cell polarization." @default.
- W2098081309 created "2016-06-24" @default.
- W2098081309 creator A5040603302 @default.
- W2098081309 date "2007-07-31" @default.
- W2098081309 modified "2023-10-17" @default.
- W2098081309 title "Scratch and See" @default.
- W2098081309 doi "https://doi.org/10.1126/stke.3982007tw283" @default.
- W2098081309 hasPublicationYear "2007" @default.
- W2098081309 type Work @default.
- W2098081309 sameAs 2098081309 @default.
- W2098081309 citedByCount "1" @default.
- W2098081309 crossrefType "journal-article" @default.
- W2098081309 hasAuthorship W2098081309A5040603302 @default.
- W2098081309 hasConcept C10447061 @default.
- W2098081309 hasConcept C107300179 @default.
- W2098081309 hasConcept C11960822 @default.
- W2098081309 hasConcept C121608353 @default.
- W2098081309 hasConcept C137620995 @default.
- W2098081309 hasConcept C137738243 @default.
- W2098081309 hasConcept C1491633281 @default.
- W2098081309 hasConcept C151166631 @default.
- W2098081309 hasConcept C182996813 @default.
- W2098081309 hasConcept C184235292 @default.
- W2098081309 hasConcept C185592680 @default.
- W2098081309 hasConcept C2775928862 @default.
- W2098081309 hasConcept C2778237340 @default.
- W2098081309 hasConcept C2780040266 @default.
- W2098081309 hasConcept C29537977 @default.
- W2098081309 hasConcept C49353375 @default.
- W2098081309 hasConcept C526805850 @default.
- W2098081309 hasConcept C53645450 @default.
- W2098081309 hasConcept C54355233 @default.
- W2098081309 hasConcept C55493867 @default.
- W2098081309 hasConcept C62478195 @default.
- W2098081309 hasConcept C86803240 @default.
- W2098081309 hasConcept C95444343 @default.
- W2098081309 hasConceptScore W2098081309C10447061 @default.
- W2098081309 hasConceptScore W2098081309C107300179 @default.
- W2098081309 hasConceptScore W2098081309C11960822 @default.
- W2098081309 hasConceptScore W2098081309C121608353 @default.
- W2098081309 hasConceptScore W2098081309C137620995 @default.
- W2098081309 hasConceptScore W2098081309C137738243 @default.
- W2098081309 hasConceptScore W2098081309C1491633281 @default.
- W2098081309 hasConceptScore W2098081309C151166631 @default.
- W2098081309 hasConceptScore W2098081309C182996813 @default.
- W2098081309 hasConceptScore W2098081309C184235292 @default.
- W2098081309 hasConceptScore W2098081309C185592680 @default.
- W2098081309 hasConceptScore W2098081309C2775928862 @default.
- W2098081309 hasConceptScore W2098081309C2778237340 @default.
- W2098081309 hasConceptScore W2098081309C2780040266 @default.
- W2098081309 hasConceptScore W2098081309C29537977 @default.
- W2098081309 hasConceptScore W2098081309C49353375 @default.
- W2098081309 hasConceptScore W2098081309C526805850 @default.
- W2098081309 hasConceptScore W2098081309C53645450 @default.
- W2098081309 hasConceptScore W2098081309C54355233 @default.
- W2098081309 hasConceptScore W2098081309C55493867 @default.
- W2098081309 hasConceptScore W2098081309C62478195 @default.
- W2098081309 hasConceptScore W2098081309C86803240 @default.
- W2098081309 hasConceptScore W2098081309C95444343 @default.
- W2098081309 hasIssue "398" @default.
- W2098081309 hasLocation W20980813091 @default.
- W2098081309 hasOpenAccess W2098081309 @default.
- W2098081309 hasPrimaryLocation W20980813091 @default.
- W2098081309 hasRelatedWork W1968966133 @default.
- W2098081309 hasRelatedWork W1970948460 @default.
- W2098081309 hasRelatedWork W2014457703 @default.
- W2098081309 hasRelatedWork W2098081309 @default.
- W2098081309 hasRelatedWork W2102366997 @default.
- W2098081309 hasRelatedWork W2103455712 @default.
- W2098081309 hasRelatedWork W2155002380 @default.
- W2098081309 hasRelatedWork W2262730689 @default.
- W2098081309 hasRelatedWork W2370215344 @default.
- W2098081309 hasRelatedWork W2470782946 @default.
- W2098081309 hasVolume "2007" @default.
- W2098081309 isParatext "false" @default.
- W2098081309 isRetracted "false" @default.
- W2098081309 magId "2098081309" @default.
- W2098081309 workType "article" @default.