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• W2098259689 endingPage "e6964" @default.
• W2098259689 startingPage "e6964" @default.
• W2098259689 abstract "To avoid molecular damage of biomolecules due to oxidation, all cells have evolved constitutive and responsive systems to mitigate and repair chemical modifications. Archaea have adapted to some of the most extreme environments known to support life, including highly oxidizing conditions. However, in comparison to bacteria and eukaryotes, relatively little is known about the biology and biochemistry of archaea in response to changing conditions and repair of oxidative damage. In this study transcriptome, proteome, and chemical reactivity analyses of hydrogen peroxide (H2O2) induced oxidative stress in Sulfolobus solfataricus (P2) were conducted. Microarray analysis of mRNA expression showed that 102 transcripts were regulated by at least 1.5 fold, 30 minutes after exposure to 30 µM H2O2. Parallel proteomic analyses using two-dimensional differential gel electrophoresis (2D-DIGE), monitored more than 800 proteins 30 and 105 minutes after exposure and found that 18 had significant changes in abundance. A recently characterized ferritin-like antioxidant protein, DPSL, was the most highly regulated species of mRNA and protein, in addition to being post-translationally modified. As expected, a number of antioxidant related mRNAs and proteins were differentially regulated. Three of these, DPSL, superoxide dismutase, and peroxiredoxin were shown to interact and likely form a novel supramolecular complex for mitigating oxidative damage. A scheme for the ability of this complex to perform multi-step reactions is presented. Despite the central role played by DPSL, cells maintained a lower level of protection after disruption of the dpsl gene, indicating a level of redundancy in the oxidative stress pathways of S. solfataricus. This work provides the first “omics” scale assessment of the oxidative stress response for an archeal organism and together with a network analysis using data from previous studies on bacteria and eukaryotes reveals evolutionarily conserved pathways where complex and overlapping defense mechanisms protect against oxygen toxicity." @default.
• W2098259689 created "2016-06-24" @default.
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• W2098259689 date "2009-09-16" @default.
• W2098259689 modified "2023-10-16" @default.
• W2098259689 title "Something Old, Something New, Something Borrowed; How the Thermoacidophilic Archaeon Sulfolobus solfataricus Responds to Oxidative Stress" @default.
• W2098259689 cites W1578854153 @default.
• W2098259689 cites W1589307419 @default.
• W2098259689 cites W1685828115 @default.
• W2098259689 cites W1706992039 @default.
• W2098259689 cites W1908973860 @default.
• W2098259689 cites W1964895626 @default.
• W2098259689 cites W1966607206 @default.
• W2098259689 cites W1970901062 @default.
• W2098259689 cites W1972083248 @default.
• W2098259689 cites W1984803435 @default.
• W2098259689 cites W1989393586 @default.
• W2098259689 cites W1991441221 @default.
• W2098259689 cites W2004188992 @default.
• W2098259689 cites W2004400264 @default.
• W2098259689 cites W2006463683 @default.
• W2098259689 cites W2012717668 @default.
• W2098259689 cites W2017265125 @default.
• W2098259689 cites W2018971148 @default.
• W2098259689 cites W2020609484 @default.
• W2098259689 cites W2021861756 @default.
• W2098259689 cites W2023706819 @default.
• W2098259689 cites W2024499248 @default.
• W2098259689 cites W2025119181 @default.
• W2098259689 cites W2025320572 @default.
• W2098259689 cites W2025773344 @default.
• W2098259689 cites W2026596261 @default.
• W2098259689 cites W2027852704 @default.
• W2098259689 cites W2033897696 @default.
• W2098259689 cites W2039655701 @default.
• W2098259689 cites W2039862991 @default.
• W2098259689 cites W2045790079 @default.
• W2098259689 cites W2046204981 @default.
• W2098259689 cites W2046300791 @default.
• W2098259689 cites W2048333869 @default.
• W2098259689 cites W2049656973 @default.
• W2098259689 cites W2056168344 @default.
• W2098259689 cites W2056568168 @default.
• W2098259689 cites W2061501796 @default.
• W2098259689 cites W2061736316 @default.
• W2098259689 cites W2061986421 @default.
• W2098259689 cites W2068431218 @default.
• W2098259689 cites W2069480316 @default.
• W2098259689 cites W2072428701 @default.
• W2098259689 cites W2075584603 @default.
• W2098259689 cites W2075853257 @default.
• W2098259689 cites W2084237466 @default.
• W2098259689 cites W2085053205 @default.
• W2098259689 cites W2096234478 @default.
• W2098259689 cites W2096859475 @default.
• W2098259689 cites W2097094841 @default.
• W2098259689 cites W2100762672 @default.
• W2098259689 cites W2100990314 @default.
• W2098259689 cites W2104251051 @default.
• W2098259689 cites W2105959645 @default.
• W2098259689 cites W2110612352 @default.
• W2098259689 cites W2115166150 @default.
• W2098259689 cites W2118365328 @default.
• W2098259689 cites W2120805921 @default.
• W2098259689 cites W2124954099 @default.
• W2098259689 cites W2124959582 @default.
• W2098259689 cites W2125194704 @default.
• W2098259689 cites W2125378369 @default.
• W2098259689 cites W2125426688 @default.
• W2098259689 cites W2126024920 @default.
• W2098259689 cites W2126948463 @default.
• W2098259689 cites W2127242142 @default.
• W2098259689 cites W2127558534 @default.
• W2098259689 cites W2127754721 @default.
• W2098259689 cites W2128148887 @default.
• W2098259689 cites W2130909247 @default.
• W2098259689 cites W2132066544 @default.
• W2098259689 cites W2133035856 @default.
• W2098259689 cites W2133312664 @default.
• W2098259689 cites W2133790733 @default.
• W2098259689 cites W2134276209 @default.
• W2098259689 cites W2134701031 @default.
• W2098259689 cites W2134809769 @default.
• W2098259689 cites W2137531873 @default.
• W2098259689 cites W2137683543 @default.
• W2098259689 cites W2142343396 @default.

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