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• W2098288954 abstract "Purpose : To investigate how cell-cycle delays in human peripheral lymphocytes affect the expression of complex chromosome damage in metaphase following high- and low-LET radiation exposure. Materials and methods : Whole blood was irradiated in vitro with a low and a high dose of 1 GeV u -1 iron particles, 400MeV u -1 neon particles or γ-rays. Lymphocytes were cultured and metaphase cells were collected at different time points after 48-84h in culture. Interphase chromosomes were prematurely condensed using calyculin-A, either 48 or 72 h after exposure to iron particles or γ-rays. Cells in first division were analysed using a combination of FISH whole-chromosome painting and DAPI/Hoechst 33258 harlequin staining. Results : There was a delay in expression of chromosome damage in metaphase that was LET- and dose-dependant. This delay was mostly related to the late emergence of complex-type damage into metaphase. Yields of damage in PCC collected 48h after irradiation with iron particles were similar to values obtained from cells undergoing mitosis after prolonged incubation. Conclusion : The yield of high-LET radiation-induced complex chromosome damage could be underestimated when analysing metaphase cells collected at one time point after irradiation. Chemically induced PCC is a more accurate technique since problems with complicated cell-cycle delays are avoided." @default.
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• W2098288954 date "2001-01-01" @default.
• W2098288954 modified "2023-09-26" @default.
• W2098288954 title "High- and low-LET induced chromosome damage in human lymphocytes: a time-course of aberrations in metaphase and interphase" @default.
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• W2098288954 doi "https://doi.org/10.1080/0955300001003760" @default.
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