FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2098337946> ?p ?o ?g. }

• W2098337946 endingPage "45" @default.
• W2098337946 startingPage "38" @default.
• W2098337946 abstract "Many different techniques have been designed for the quantification of JAK2V617F allelic burden, sometimes producing discrepant results.JAK2V617F quantification techniques were compared among 16 centers using 11 assays based on quantitative polymerase chain reaction (with mutation-specific primers or probes, or fluorescent resonance energy transfer/melting curve analysis), allele-specific polymerase chain reaction, conventional sequencing or pyrosequencing.A first series of blinded samples (granulocyte DNA, n=29) was analyzed. Seven assays (12 centers) reported values inside the mean +/- 2SD; the mean coefficient of variation was 31%. Sequencing techniques lacked sensitivity, and strong discrepancies were observed with four techniques, which could be attributed to inadequate standards or to different modes of expression of results. Indeed, quantification of JAK2V617F in relation to another control gene produced higher than expected values, suggesting the possibility of more than two JAK2 copies/cell. After calibration of assays with common 1% to 100% JAK2V617F standards (dilutions of UKE-1 cells in normal leukocytes), 14 centers tested ten new samples. JAK2V617F allelic burdens greater or equal than 1% were then reliably quantified by five techniques -- one allele specific-polymerase chain reaction and four TaqMan allele-specific quantitative polymerase chain reaction assays, including one previously giving results outside the mean +/- 2SD -- with a lower mean coefficient of variation (21%). Of these, only the two TaqMan allele-specific quantitative polymerase chain reaction assays with primer-based specificity could detect 0.2% JAK2V617F.Techniques expressing the allelic burden as JAK2V617F/total JAK2 and using a common set of standards produced similar quantification results but with variable sensitivity. Calibration to a reference standard improved reproducibility." @default.
• W2098337946 created "2016-06-24" @default.
• W2098337946 creator A5001646221 @default.
• W2098337946 creator A5004553363 @default.
• W2098337946 creator A5010203773 @default.
• W2098337946 creator A5011879422 @default.
• W2098337946 creator A5019037633 @default.
• W2098337946 creator A5019208096 @default.
• W2098337946 creator A5026675862 @default.
• W2098337946 creator A5027336256 @default.
• W2098337946 creator A5027542373 @default.
• W2098337946 creator A5029918265 @default.
• W2098337946 creator A5031749209 @default.
• W2098337946 creator A5036710633 @default.
• W2098337946 creator A5041088127 @default.
• W2098337946 creator A5042381475 @default.
• W2098337946 creator A5042691362 @default.
• W2098337946 creator A5047337692 @default.
• W2098337946 creator A5050358937 @default.
• W2098337946 creator A5052150142 @default.
• W2098337946 creator A5052933885 @default.
• W2098337946 creator A5056157634 @default.
• W2098337946 creator A5073081349 @default.
• W2098337946 creator A5080172381 @default.
• W2098337946 creator A5084653587 @default.
• W2098337946 creator A5089969812 @default.
• W2098337946 date "2009-01-01" @default.
• W2098337946 modified "2023-09-26" @default.
• W2098337946 title "Concordance of assays designed for the quantification of JAK2V617F: a multicenter study" @default.
• W2098337946 cites W1498640978 @default.
• W2098337946 cites W1554559727 @default.
• W2098337946 cites W1964991616 @default.
• W2098337946 cites W1970553871 @default.
• W2098337946 cites W1980568348 @default.
• W2098337946 cites W1985177017 @default.
• W2098337946 cites W1988060134 @default.
• W2098337946 cites W1989188541 @default.
• W2098337946 cites W1994580828 @default.
• W2098337946 cites W2000081002 @default.
• W2098337946 cites W2014268597 @default.
• W2098337946 cites W2022930434 @default.
• W2098337946 cites W2030625475 @default.
• W2098337946 cites W2031932184 @default.
• W2098337946 cites W2034610539 @default.
• W2098337946 cites W2043737324 @default.
• W2098337946 cites W2045411075 @default.
• W2098337946 cites W2049145190 @default.
• W2098337946 cites W2066465348 @default.
• W2098337946 cites W2075970562 @default.
• W2098337946 cites W2099264304 @default.
• W2098337946 cites W2110190127 @default.
• W2098337946 cites W2114773824 @default.
• W2098337946 cites W2129398278 @default.
• W2098337946 cites W2156482024 @default.
• W2098337946 cites W2162013503 @default.
• W2098337946 cites W2548905472 @default.
• W2098337946 cites W351264868 @default.
• W2098337946 cites W651165243 @default.
• W2098337946 doi "https://doi.org/10.3324/haematol.13486" @default.
• W2098337946 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2625411" @default.
• W2098337946 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19001280" @default.
• W2098337946 hasPublicationYear "2009" @default.
• W2098337946 type Work @default.
• W2098337946 sameAs 2098337946 @default.
• W2098337946 citedByCount "75" @default.
• W2098337946 countsByYear W20983379462012 @default.
• W2098337946 countsByYear W20983379462013 @default.
• W2098337946 countsByYear W20983379462014 @default.
• W2098337946 countsByYear W20983379462015 @default.
• W2098337946 countsByYear W20983379462016 @default.
• W2098337946 countsByYear W20983379462017 @default.
• W2098337946 countsByYear W20983379462018 @default.
• W2098337946 countsByYear W20983379462019 @default.
• W2098337946 countsByYear W20983379462020 @default.
• W2098337946 countsByYear W20983379462021 @default.
• W2098337946 countsByYear W20983379462022 @default.
• W2098337946 countsByYear W20983379462023 @default.
• W2098337946 crossrefType "journal-article" @default.
• W2098337946 hasAuthorship W2098337946A5001646221 @default.
• W2098337946 hasAuthorship W2098337946A5004553363 @default.
• W2098337946 hasAuthorship W2098337946A5010203773 @default.
• W2098337946 hasAuthorship W2098337946A5011879422 @default.
• W2098337946 hasAuthorship W2098337946A5019037633 @default.
• W2098337946 hasAuthorship W2098337946A5019208096 @default.
• W2098337946 hasAuthorship W2098337946A5026675862 @default.
• W2098337946 hasAuthorship W2098337946A5027336256 @default.
• W2098337946 hasAuthorship W2098337946A5027542373 @default.
• W2098337946 hasAuthorship W2098337946A5029918265 @default.
• W2098337946 hasAuthorship W2098337946A5031749209 @default.
• W2098337946 hasAuthorship W2098337946A5036710633 @default.
• W2098337946 hasAuthorship W2098337946A5041088127 @default.
• W2098337946 hasAuthorship W2098337946A5042381475 @default.
• W2098337946 hasAuthorship W2098337946A5042691362 @default.
• W2098337946 hasAuthorship W2098337946A5047337692 @default.
• W2098337946 hasAuthorship W2098337946A5050358937 @default.
• W2098337946 hasAuthorship W2098337946A5052150142 @default.
• W2098337946 hasAuthorship W2098337946A5052933885 @default.
• W2098337946 hasAuthorship W2098337946A5056157634 @default.

• next