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• W2098366954 abstract "Microarray datasets are a rich source of information in nutritional investigation. Targeted mining of microarray data following initial, non-biased bioinformatic analysis can provide key insight into specific genes and metabolic processes of interest. Microarrays from human adipocytes were examined to explore the effects of macrophage secretions on the expression of the G-protein-coupled receptor (GPR) genes that encode fatty acid receptors/sensors. Exposure of the adipocytes to macrophage-conditioned medium for 4 or 24 h had no effect on GPR40 and GPR43 expression, but there was a marked stimulation of GPR84 expression (receptor for medium-chain fatty acids), the mRNA level increasing 13·5-fold at 24 h relative to unconditioned medium. Importantly, expression of GPR120, which encodes an n-3 PUFA receptor/sensor, was strongly inhibited by the conditioned medium (15-fold decrease in mRNA at 24 h). Macrophage secretions have major effects on the expression of fatty acid receptor/sensor genes in human adipocytes, which may lead to an augmentation of the inflammatory response in adipose tissue in obesity." @default.
• W2098366954 created "2016-06-24" @default.
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• W2098366954 date "2012-06-01" @default.
• W2098366954 modified "2023-10-03" @default.
• W2098366954 title "Mining microarray datasets in nutrition: expression of the <i>GPR120</i> (<i>n</i>-3 fatty acid receptor/sensor) gene is down-regulated in human adipocytes by macrophage secretions" @default.
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• W2098366954 doi "https://doi.org/10.1017/jns.2012.3" @default.
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