Matches in SemOpenAlex for { <https://semopenalex.org/work/W2098455947> ?p ?o ?g. }
- W2098455947 endingPage "3195" @default.
- W2098455947 startingPage "3185" @default.
- W2098455947 abstract "ABSTRACT A method to study the function of individual African swine fever virus (ASFV) gene products utilizing the Escherichia coli lac repressor-operator system has been developed. Recombinant viruses containing both the lacI gene encoding the lac repressor and a strong virus late promoter modified by the insertion of one or two copies of the lac operator sequence at various positions were constructed. The ability of each modified promoter to regulate expression of the firefly luciferase gene was assayed in the presence and in the absence of the inducer isopropyl β- d -thiogalactoside (IPTG). Induction and repression of gene activity were dependent on the position(s) of the operator(s) with respect to the promoter and on the number of operators inserted. The ability of this system to regulate the expression of ASFV genes was analyzed by constructing a recombinant virus inducibly expressing the major capsid protein p72. Electron microscopy analysis revealed that under nonpermissive conditions, electron-dense membrane-like structures accumulated in the viral factories and capsid formation was inhibited. Induction of p72 expression allowed the progressive building of the capsid on these structures, leading to assembly of ASFV particles. The results of this report demonstrate that the transferred inducible expression system is a powerful tool for analyzing the function of ASFV genes." @default.
- W2098455947 created "2016-06-24" @default.
- W2098455947 creator A5014205573 @default.
- W2098455947 creator A5046963843 @default.
- W2098455947 creator A5066051519 @default.
- W2098455947 creator A5081996486 @default.
- W2098455947 date "1998-04-01" @default.
- W2098455947 modified "2023-10-18" @default.
- W2098455947 title "Inducible Gene Expression from African Swine Fever Virus Recombinants: Analysis of the Major Capsid Protein p72" @default.
- W2098455947 cites W1242757451 @default.
- W2098455947 cites W1486321123 @default.
- W2098455947 cites W1494593126 @default.
- W2098455947 cites W1523665785 @default.
- W2098455947 cites W1538361043 @default.
- W2098455947 cites W1541195468 @default.
- W2098455947 cites W1542837477 @default.
- W2098455947 cites W1567981435 @default.
- W2098455947 cites W1570685643 @default.
- W2098455947 cites W1578860231 @default.
- W2098455947 cites W1595238427 @default.
- W2098455947 cites W167697669 @default.
- W2098455947 cites W1759660421 @default.
- W2098455947 cites W1864893929 @default.
- W2098455947 cites W1943717348 @default.
- W2098455947 cites W1967013096 @default.
- W2098455947 cites W1970385134 @default.
- W2098455947 cites W1973720950 @default.
- W2098455947 cites W1978237557 @default.
- W2098455947 cites W1980991323 @default.
- W2098455947 cites W1990763318 @default.
- W2098455947 cites W1993580305 @default.
- W2098455947 cites W1995052530 @default.
- W2098455947 cites W1996992296 @default.
- W2098455947 cites W1997755405 @default.
- W2098455947 cites W1998917046 @default.
- W2098455947 cites W2001315239 @default.
- W2098455947 cites W2004073738 @default.
- W2098455947 cites W2010678227 @default.
- W2098455947 cites W2014572189 @default.
- W2098455947 cites W2014971990 @default.
- W2098455947 cites W2018289835 @default.
- W2098455947 cites W2018778009 @default.
- W2098455947 cites W2021213516 @default.
- W2098455947 cites W2023051334 @default.
- W2098455947 cites W2024658606 @default.
- W2098455947 cites W2027792116 @default.
- W2098455947 cites W2042107008 @default.
- W2098455947 cites W2055588321 @default.
- W2098455947 cites W2061293103 @default.
- W2098455947 cites W2072971665 @default.
- W2098455947 cites W2076300996 @default.
- W2098455947 cites W2078638959 @default.
- W2098455947 cites W2079896651 @default.
- W2098455947 cites W2087759132 @default.
- W2098455947 cites W2092923090 @default.
- W2098455947 cites W2094559945 @default.
- W2098455947 cites W2100837269 @default.
- W2098455947 cites W2109794591 @default.
- W2098455947 cites W2113222286 @default.
- W2098455947 cites W2127088139 @default.
- W2098455947 cites W2128635872 @default.
- W2098455947 cites W2139674974 @default.
- W2098455947 cites W2146138171 @default.
- W2098455947 cites W2149664246 @default.
- W2098455947 cites W2163576959 @default.
- W2098455947 cites W2404067333 @default.
- W2098455947 cites W394299496 @default.
- W2098455947 cites W4293247451 @default.
- W2098455947 doi "https://doi.org/10.1128/jvi.72.4.3185-3195.1998" @default.
- W2098455947 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/109780" @default.
- W2098455947 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9580160" @default.
- W2098455947 hasPublicationYear "1998" @default.
- W2098455947 type Work @default.
- W2098455947 sameAs 2098455947 @default.
- W2098455947 citedByCount "71" @default.
- W2098455947 countsByYear W20984559472012 @default.
- W2098455947 countsByYear W20984559472013 @default.
- W2098455947 countsByYear W20984559472015 @default.
- W2098455947 countsByYear W20984559472016 @default.
- W2098455947 countsByYear W20984559472017 @default.
- W2098455947 countsByYear W20984559472018 @default.
- W2098455947 countsByYear W20984559472020 @default.
- W2098455947 countsByYear W20984559472021 @default.
- W2098455947 countsByYear W20984559472022 @default.
- W2098455947 countsByYear W20984559472023 @default.
- W2098455947 crossrefType "journal-article" @default.
- W2098455947 hasAuthorship W2098455947A5014205573 @default.
- W2098455947 hasAuthorship W2098455947A5046963843 @default.
- W2098455947 hasAuthorship W2098455947A5066051519 @default.
- W2098455947 hasAuthorship W2098455947A5081996486 @default.
- W2098455947 hasBestOaLocation W20984559471 @default.
- W2098455947 hasConcept C101762097 @default.
- W2098455947 hasConcept C104317684 @default.
- W2098455947 hasConcept C113629113 @default.
- W2098455947 hasConcept C150194340 @default.
- W2098455947 hasConcept C153911025 @default.
- W2098455947 hasConcept C158448853 @default.
- W2098455947 hasConcept C159047783 @default.