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• W2098536639 startingPage "3814" @default.
• W2098536639 abstract "The siderophore and virulence factor yersiniabactin is produced by Pseudomonas syringae. Yersiniabactin was originally detected by high-pressure liquid chromatography (HPLC); commonly used PCR tests proved ineffective. Yersiniabactin production in P. syringae correlated with the possession of irp1 located in a predicted yersiniabactin locus. Three similarly divergent yersiniabactin locus groups were determined: the Yersinia pestis group, the P. syringae group, and the Photorhabdus luminescens group; yersiniabactin locus organization is similar in P. syringae and P. luminescens. In P. syringae pv. tomato DC3000, the locus has a high GC content (63.4% compared with 58.4% for the chromosome and 60.1% and 60.7% for adjacent regions) but it lacks high-pathogenicity-island features, such as the insertion in a tRNA locus, the integrase, and insertion sequence elements. In P. syringae pv. tomato DC3000 and pv. phaseolicola 1448A, the locus lies between homologues of Psyr_2284 and Psyr_2285 of P. syringae pv. syringae B728a, which lacks the locus. Among tested pseudomonads, a PCR test specific to two yersiniabactin locus groups detected a locus in genospecies 3, 7, and 8 of P. syringae, and DNA hybridization within P. syringae also detected a locus in the pathovars phaseolicola and glycinea. The PCR and HPLC methods enabled analysis of nonpathogenic Escherichia coli. HPLC-proven yersiniabactin-producing E. coli lacked modifications found in irp1 and irp2 in the human pathogen CFT073, and it is not clear whether CFT073 produces yersiniabactin. The study provides clues about the evolution and dispersion of yersiniabactin genes. It describes methods to detect and study yersiniabactin producers, even where genes have evolved." @default.
• W2098536639 created "2016-06-24" @default.
• W2098536639 creator A5025228961 @default.
• W2098536639 creator A5038095913 @default.
• W2098536639 creator A5047075027 @default.
• W2098536639 date "2006-06-01" @default.
• W2098536639 modified "2023-10-06" @default.
• W2098536639 title "Yersiniabactin Production by <i>Pseudomonas syringae</i> and <i>Escherichia coli</i> , and Description of a Second Yersiniabactin Locus Evolutionary Group" @default.
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• W2098536639 doi "https://doi.org/10.1128/aem.00119-06" @default.
• W2098536639 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1489633" @default.
• W2098536639 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16751485" @default.
• W2098536639 hasPublicationYear "2006" @default.
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