FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2098714535> ?p ?o ?g. }

• W2098714535 endingPage "221" @default.
• W2098714535 startingPage "211" @default.
• W2098714535 abstract "Recent studies suggest that immature, core-glycosylated ΔF508-CFTR [the predominant mutant form of the CFTR (cystic fibrosis transmembrane conductance regulator)] can reach the plasma membrane under some conditions. In the present study we investigated this possibility since it has implications for understanding how therapeutics rescue the trafficking of mutant CFTR and perhaps other misfolded proteins. Core-glycosylated CFTR was labelled and pulled down on streptavidin beads after exposure to sulfo-NHS-SS-biotin [biotin attached to a reactive NHS (N-hydroxysuccinimide) ester with a disulfide spacer; molecular mass=606.7 Da]; however, intracellular proteins were also detected in the precipitates. When the R domain of CFTR was expressed in the cytosol of BHK (baby-hamster kidney) cells as a soluble polypeptide it was also labelled after surface biotinylation and pulled down on streptavidin beads. Intracellular biotinylation was reduced when cells were treated with sulfo-NHS-LC-biotin (biotin attached to a reactive NHS ester with an aminocaproic acid spacer) or sulfo-NHS-PEO12-biotin [biotin attached to a reactive NHS ester with a poly(ethylene glycol) spacer], but the reduction could be explained by the lower reactivity of these reagents. The R domain was detected on Western blots after loading &lt;0.25% of the pulldown sample (∼0.01% of total lysate protein), a fraction that could be ascribed to cells that were permeable to ethidium homodimer-1 (molecular mass=856.8 Da) and propidium iodide (molecular mass=668.6 Da). When BHK cells were incubated at 29 °C to rescue ΔF508-CFTR trafficking, and then biotinylated and sorted to remove permeable cells, labelling of core-glycosylated ΔF508-CFTR was no longer detected although a weak signal was still observed using CFBE (cystic fibrosis bronchial epithelial) cells. These results suggest that there is weak surface expression of immature ΔF508-CFTR on airway epithelial cells and demonstrate the need to remove permeable cells when studying CFTR glycoforms by surface biotinylation." @default.
• W2098714535 created "2016-06-24" @default.
• W2098714535 creator A5004357421 @default.
• W2098714535 creator A5005230910 @default.
• W2098714535 creator A5056371233 @default.
• W2098714535 date "2009-03-13" @default.
• W2098714535 modified "2023-09-26" @default.
• W2098714535 title "Trafficking of immature ΔF508-CFTR to the plasma membrane and its detection by biotinylation" @default.
• W2098714535 cites W1973958173 @default.
• W2098714535 cites W1975971473 @default.
• W2098714535 cites W1976507289 @default.
• W2098714535 cites W1985017562 @default.
• W2098714535 cites W2012602248 @default.
• W2098714535 cites W2014319545 @default.
• W2098714535 cites W2021348896 @default.
• W2098714535 cites W2047746505 @default.
• W2098714535 cites W2057625458 @default.
• W2098714535 cites W2061816753 @default.
• W2098714535 cites W2072558851 @default.
• W2098714535 cites W2076860446 @default.
• W2098714535 cites W2079234445 @default.
• W2098714535 cites W2086750244 @default.
• W2098714535 cites W2093904931 @default.
• W2098714535 cites W2103130264 @default.
• W2098714535 cites W2122280687 @default.
• W2098714535 cites W2125460059 @default.
• W2098714535 cites W2136656278 @default.
• W2098714535 cites W2152355434 @default.
• W2098714535 cites W2170675946 @default.
• W2098714535 cites W2262325900 @default.
• W2098714535 cites W2342658150 @default.
• W2098714535 cites W84336447 @default.
• W2098714535 doi "https://doi.org/10.1042/bj20081869" @default.
• W2098714535 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19053947" @default.
• W2098714535 hasPublicationYear "2009" @default.
• W2098714535 type Work @default.
• W2098714535 sameAs 2098714535 @default.
• W2098714535 citedByCount "26" @default.
• W2098714535 countsByYear W20987145352012 @default.
• W2098714535 countsByYear W20987145352013 @default.
• W2098714535 countsByYear W20987145352014 @default.
• W2098714535 countsByYear W20987145352015 @default.
• W2098714535 countsByYear W20987145352016 @default.
• W2098714535 countsByYear W20987145352018 @default.
• W2098714535 countsByYear W20987145352019 @default.
• W2098714535 countsByYear W20987145352021 @default.
• W2098714535 countsByYear W20987145352022 @default.
• W2098714535 crossrefType "journal-article" @default.
• W2098714535 hasAuthorship W2098714535A5004357421 @default.
• W2098714535 hasAuthorship W2098714535A5005230910 @default.
• W2098714535 hasAuthorship W2098714535A5056371233 @default.
• W2098714535 hasConcept C104317684 @default.
• W2098714535 hasConcept C1491633281 @default.
• W2098714535 hasConcept C153911025 @default.
• W2098714535 hasConcept C185592680 @default.
• W2098714535 hasConcept C204628709 @default.
• W2098714535 hasConcept C2776190903 @default.
• W2098714535 hasConcept C2777893369 @default.
• W2098714535 hasConcept C2778204606 @default.
• W2098714535 hasConcept C2778428886 @default.
• W2098714535 hasConcept C33263850 @default.
• W2098714535 hasConcept C55493867 @default.
• W2098714535 hasConcept C79879829 @default.
• W2098714535 hasConcept C85265743 @default.
• W2098714535 hasConcept C86803240 @default.
• W2098714535 hasConceptScore W2098714535C104317684 @default.
• W2098714535 hasConceptScore W2098714535C1491633281 @default.
• W2098714535 hasConceptScore W2098714535C153911025 @default.
• W2098714535 hasConceptScore W2098714535C185592680 @default.
• W2098714535 hasConceptScore W2098714535C204628709 @default.
• W2098714535 hasConceptScore W2098714535C2776190903 @default.
• W2098714535 hasConceptScore W2098714535C2777893369 @default.
• W2098714535 hasConceptScore W2098714535C2778204606 @default.
• W2098714535 hasConceptScore W2098714535C2778428886 @default.
• W2098714535 hasConceptScore W2098714535C33263850 @default.
• W2098714535 hasConceptScore W2098714535C55493867 @default.
• W2098714535 hasConceptScore W2098714535C79879829 @default.
• W2098714535 hasConceptScore W2098714535C85265743 @default.
• W2098714535 hasConceptScore W2098714535C86803240 @default.
• W2098714535 hasIssue "1" @default.
• W2098714535 hasLocation W20987145351 @default.
• W2098714535 hasLocation W20987145352 @default.
• W2098714535 hasOpenAccess W2098714535 @default.
• W2098714535 hasPrimaryLocation W20987145351 @default.
• W2098714535 hasRelatedWork W1511280725 @default.
• W2098714535 hasRelatedWork W2018310880 @default.
• W2098714535 hasRelatedWork W202670695 @default.
• W2098714535 hasRelatedWork W2098714535 @default.
• W2098714535 hasRelatedWork W2158943306 @default.
• W2098714535 hasRelatedWork W2396522900 @default.
• W2098714535 hasRelatedWork W2462922943 @default.
• W2098714535 hasRelatedWork W2746163646 @default.
• W2098714535 hasRelatedWork W2767828069 @default.
• W2098714535 hasRelatedWork W4297992181 @default.
• W2098714535 hasVolume "419" @default.
• W2098714535 isParatext "false" @default.
• W2098714535 isRetracted "false" @default.
• W2098714535 magId "2098714535" @default.

• next