FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2098840211> ?p ?o ?g. }

• W2098840211 endingPage "e42704" @default.
• W2098840211 startingPage "e42704" @default.
• W2098840211 abstract "Promoter methylation profiles are proposed as potential prognosis and/or diagnosis biomarkers in cervical cancer. Up to now, little is known about the promoter methylation profile and expression pattern of stem cell (SC) markers during tumor development. In this study, we were interested to identify SC genes methylation profiles during cervical carcinogenesis. A genome-wide promoter methylation screening revealed a strong hypermethylation of Undifferentiated cell Transcription Factor 1 (UTF1) promoter in cervical cancer in comparison with normal ectocervix. By direct bisulfite pyrosequencing of DNA isolated from liquid-based cytological samples, we showed that UTF1 promoter methylation increases with lesion severity, the highest level of methylation being found in carcinoma. This hypermethylation was associated with increased UTF1 mRNA and protein expression. By using quantitative RT-PCR and Western Blot, we showed that both UTF1 mRNA and protein are present in epithelial cancer cell lines, even in the absence of its two main described regulators Oct4A and Sox2. Moreover, by immunofluorescence, we confirmed the nuclear localisation of UTF1 in cell lines. Surprisingly, direct bisulfite pyrosequencing revealed that the inhibition of DNA methyltransferase by 5-aza-2'-deoxycytidine was associated with decreased UTF1 gene methylation and expression in two cervical cancer cell lines of the four tested. These findings strongly suggest that UTF1 promoter methylation profile might be a useful biomarker for cervical cancer diagnosis and raise the questions of its role during epithelial carcinogenesis and of the mechanisms regulating its expression." @default.
• W2098840211 created "2016-06-24" @default.
• W2098840211 creator A5014115827 @default.
• W2098840211 creator A5015007361 @default.
• W2098840211 creator A5018869706 @default.
• W2098840211 creator A5025624026 @default.
• W2098840211 creator A5038483344 @default.
• W2098840211 creator A5043108540 @default.
• W2098840211 creator A5048998883 @default.
• W2098840211 creator A5065632278 @default.
• W2098840211 creator A5069118692 @default.
• W2098840211 creator A5078420969 @default.
• W2098840211 creator A5079802157 @default.
• W2098840211 date "2012-08-03" @default.
• W2098840211 modified "2023-10-14" @default.
• W2098840211 title "Aberrant Promoter Methylation and Expression of UTF1 during Cervical Carcinogenesis" @default.
• W2098840211 cites W1518200090 @default.
• W2098840211 cites W1558096440 @default.
• W2098840211 cites W1877598057 @default.
• W2098840211 cites W1884113824 @default.
• W2098840211 cites W1963744267 @default.
• W2098840211 cites W1966676297 @default.
• W2098840211 cites W1970818296 @default.
• W2098840211 cites W1981989535 @default.
• W2098840211 cites W1985089231 @default.
• W2098840211 cites W1990789249 @default.
• W2098840211 cites W1995640339 @default.
• W2098840211 cites W1997793743 @default.
• W2098840211 cites W2003592185 @default.
• W2098840211 cites W2007139340 @default.
• W2098840211 cites W2007155094 @default.
• W2098840211 cites W2008284862 @default.
• W2098840211 cites W2013211434 @default.
• W2098840211 cites W2013531495 @default.
• W2098840211 cites W2020483546 @default.
• W2098840211 cites W2023942950 @default.
• W2098840211 cites W2027162391 @default.
• W2098840211 cites W2036190704 @default.
• W2098840211 cites W2060375833 @default.
• W2098840211 cites W2060889804 @default.
• W2098840211 cites W2062076781 @default.
• W2098840211 cites W2063941640 @default.
• W2098840211 cites W2064830547 @default.
• W2098840211 cites W2067083508 @default.
• W2098840211 cites W2067781054 @default.
• W2098840211 cites W2068509272 @default.
• W2098840211 cites W2071110150 @default.
• W2098840211 cites W2073393965 @default.
• W2098840211 cites W2079590675 @default.
• W2098840211 cites W2082051295 @default.
• W2098840211 cites W2088596095 @default.
• W2098840211 cites W2093203122 @default.
• W2098840211 cites W2094454538 @default.
• W2098840211 cites W2095799750 @default.
• W2098840211 cites W2102085111 @default.
• W2098840211 cites W2102140165 @default.
• W2098840211 cites W2114918609 @default.
• W2098840211 cites W2116123637 @default.
• W2098840211 cites W2116228913 @default.
• W2098840211 cites W2117692326 @default.
• W2098840211 cites W2120571560 @default.
• W2098840211 cites W2123174009 @default.
• W2098840211 cites W2123473989 @default.
• W2098840211 cites W2123557395 @default.
• W2098840211 cites W2126376889 @default.
• W2098840211 cites W2130673887 @default.
• W2098840211 cites W2136438175 @default.
• W2098840211 cites W2141064505 @default.
• W2098840211 cites W2143328224 @default.
• W2098840211 cites W2153562609 @default.
• W2098840211 cites W2156365913 @default.
• W2098840211 cites W2162873248 @default.
• W2098840211 cites W2163125553 @default.
• W2098840211 cites W2165587740 @default.
• W2098840211 cites W2170682201 @default.
• W2098840211 cites W90661041 @default.
• W2098840211 doi "https://doi.org/10.1371/journal.pone.0042704" @default.
• W2098840211 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3411846" @default.
• W2098840211 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22880087" @default.
• W2098840211 hasPublicationYear "2012" @default.
• W2098840211 type Work @default.
• W2098840211 sameAs 2098840211 @default.
• W2098840211 citedByCount "24" @default.
• W2098840211 countsByYear W20988402112013 @default.
• W2098840211 countsByYear W20988402112014 @default.
• W2098840211 countsByYear W20988402112015 @default.
• W2098840211 countsByYear W20988402112016 @default.
• W2098840211 countsByYear W20988402112017 @default.
• W2098840211 countsByYear W20988402112018 @default.
• W2098840211 countsByYear W20988402112019 @default.
• W2098840211 countsByYear W20988402112021 @default.
• W2098840211 countsByYear W20988402112022 @default.
• W2098840211 countsByYear W20988402112023 @default.
• W2098840211 crossrefType "journal-article" @default.
• W2098840211 hasAuthorship W2098840211A5014115827 @default.
• W2098840211 hasAuthorship W2098840211A5015007361 @default.
• W2098840211 hasAuthorship W2098840211A5018869706 @default.
• W2098840211 hasAuthorship W2098840211A5025624026 @default.

• next