FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2098943921> ?p ?o ?g. }

• W2098943921 endingPage "585" @default.
• W2098943921 startingPage "571" @default.
• W2098943921 abstract "During amino acid starvation, bacterial cells rapidly synthesize the nucleotides (p)ppGpp, causing a massive re-programming of the transcriptional profile known as the stringent response. The (p)ppGpp synthase RelA is activated by ribosomes harboring an uncharged tRNA at the A site. It is unclear whether synthesis occurs while RelA is bound to the ribosome or free in the cytoplasm. We present a study of three Escherichia coli strains, each expressing a different RelA-fluorescent protein (RelA-FP) construct: RelA-YFP, RelA-mEos2 and RelA-Dendra2. Single-molecule localization and tracking studies were carried out under normal growth conditions and during amino acid starvation. Study of three labeling schemes enabled us to assess potential problems with FP labeling of RelA. The diffusive trajectories and axial spatial distributions indicate that amino acid starvation induces net binding of all three RelA-FP constructs to 70S ribosomes. The data are most consistent with a model in which RelA synthesizes (p)ppGpp while bound to the 70S ribosome. We suggest a 'short hopping time' model of RelA activity during starvation. Our results contradict an earlier study of RelA-Dendra2 diffusion that inferred off-ribosome synthesis of (p)ppGpp. The reasons for the discrepancy remain unclear." @default.
• W2098943921 created "2016-06-24" @default.
• W2098943921 creator A5028981049 @default.
• W2098943921 creator A5056929785 @default.
• W2098943921 creator A5068627804 @default.
• W2098943921 creator A5076256227 @default.
• W2098943921 creator A5077564904 @default.
• W2098943921 creator A5086664284 @default.
• W2098943921 date "2015-11-10" @default.
• W2098943921 modified "2023-10-15" @default.
• W2098943921 title "Effects of amino acid starvation on RelA diffusive behavior in live<i>E</i><i>scherichia coli</i>" @default.
• W2098943921 cites W1781531090 @default.
• W2098943921 cites W1883779479 @default.
• W2098943921 cites W1905117713 @default.
• W2098943921 cites W1917921565 @default.
• W2098943921 cites W1967826482 @default.
• W2098943921 cites W1968414070 @default.
• W2098943921 cites W1971507610 @default.
• W2098943921 cites W1988278060 @default.
• W2098943921 cites W1990823004 @default.
• W2098943921 cites W1994944889 @default.
• W2098943921 cites W2001221216 @default.
• W2098943921 cites W2004235182 @default.
• W2098943921 cites W2006257982 @default.
• W2098943921 cites W2008778576 @default.
• W2098943921 cites W2022437289 @default.
• W2098943921 cites W2034876354 @default.
• W2098943921 cites W2038871597 @default.
• W2098943921 cites W2042629182 @default.
• W2098943921 cites W2057349693 @default.
• W2098943921 cites W2060177007 @default.
• W2098943921 cites W2095719211 @default.
• W2098943921 cites W2097101221 @default.
• W2098943921 cites W2105028012 @default.
• W2098943921 cites W2109335024 @default.
• W2098943921 cites W2114085934 @default.
• W2098943921 cites W2115020206 @default.
• W2098943921 cites W2124254415 @default.
• W2098943921 cites W2125301321 @default.
• W2098943921 cites W2136017260 @default.
• W2098943921 cites W2138611595 @default.
• W2098943921 cites W2148579902 @default.
• W2098943921 cites W2164856850 @default.
• W2098943921 cites W2167892316 @default.
• W2098943921 cites W81658165 @default.
• W2098943921 doi "https://doi.org/10.1111/mmi.13252" @default.
• W2098943921 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4962531" @default.
• W2098943921 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26480956" @default.
• W2098943921 hasPublicationYear "2015" @default.
• W2098943921 type Work @default.
• W2098943921 sameAs 2098943921 @default.
• W2098943921 citedByCount "28" @default.
• W2098943921 countsByYear W20989439212016 @default.
• W2098943921 countsByYear W20989439212017 @default.
• W2098943921 countsByYear W20989439212018 @default.
• W2098943921 countsByYear W20989439212019 @default.
• W2098943921 countsByYear W20989439212020 @default.
• W2098943921 countsByYear W20989439212021 @default.
• W2098943921 countsByYear W20989439212022 @default.
• W2098943921 countsByYear W20989439212023 @default.
• W2098943921 crossrefType "journal-article" @default.
• W2098943921 hasAuthorship W2098943921A5028981049 @default.
• W2098943921 hasAuthorship W2098943921A5056929785 @default.
• W2098943921 hasAuthorship W2098943921A5068627804 @default.
• W2098943921 hasAuthorship W2098943921A5076256227 @default.
• W2098943921 hasAuthorship W2098943921A5077564904 @default.
• W2098943921 hasAuthorship W2098943921A5086664284 @default.
• W2098943921 hasBestOaLocation W20989439211 @default.
• W2098943921 hasConcept C104317684 @default.
• W2098943921 hasConcept C134018914 @default.
• W2098943921 hasConcept C153957851 @default.
• W2098943921 hasConcept C26628138 @default.
• W2098943921 hasConcept C2780606744 @default.
• W2098943921 hasConcept C3675279 @default.
• W2098943921 hasConcept C515207424 @default.
• W2098943921 hasConcept C547475151 @default.
• W2098943921 hasConcept C55493867 @default.
• W2098943921 hasConcept C67705224 @default.
• W2098943921 hasConcept C86803240 @default.
• W2098943921 hasConcept C88478588 @default.
• W2098943921 hasConcept C95444343 @default.
• W2098943921 hasConceptScore W2098943921C104317684 @default.
• W2098943921 hasConceptScore W2098943921C134018914 @default.
• W2098943921 hasConceptScore W2098943921C153957851 @default.
• W2098943921 hasConceptScore W2098943921C26628138 @default.
• W2098943921 hasConceptScore W2098943921C2780606744 @default.
• W2098943921 hasConceptScore W2098943921C3675279 @default.
• W2098943921 hasConceptScore W2098943921C515207424 @default.
• W2098943921 hasConceptScore W2098943921C547475151 @default.
• W2098943921 hasConceptScore W2098943921C55493867 @default.
• W2098943921 hasConceptScore W2098943921C67705224 @default.
• W2098943921 hasConceptScore W2098943921C86803240 @default.
• W2098943921 hasConceptScore W2098943921C88478588 @default.
• W2098943921 hasConceptScore W2098943921C95444343 @default.
• W2098943921 hasFunder F4320309911 @default.
• W2098943921 hasFunder F4320332161 @default.
• W2098943921 hasIssue "3" @default.
• W2098943921 hasLocation W20989439211 @default.

• next