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- W2098975276 abstract "An off‐line two‐dimensional high‐speed counter‐current chromatography method combined with gradient and recycling elution mode was established to isolate terpenoids and flavones from the leaves of Andrographis paniculata (Burm. f.) Nees. By using the solvent systems composed of n ‐hexane/ethyl acetate/methanol/water with different volume ratios, five compounds including roseooside, 5,4′‐dihydroxyflavonoid‐7‐ O ‐β‐ d ‐pyranglucuronatebutylester, 7,8‐dimethoxy‐2′‐hydroxy‐5‐ O ‐β‐ d ‐glucopyranosyloxyflavon, 14‐deoxyandrographiside, and andrographolide were successfully isolated. Purities of these isolated compounds were all over 95% as determined by high‐performance liquid chromatography. Their structures were identified by UV, mass spectrometry, and 1 H NMR spectroscopy. It has been demonstrated that the combination of off‐line two‐dimensional high‐speed counter‐current chromatography with different elution modes is an efficient technique to isolate compounds from complex natural product extracts." @default.
- W2098975276 created "2016-06-24" @default.
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- W2098975276 date "2015-03-24" @default.
- W2098975276 modified "2023-10-10" @default.
- W2098975276 title "Separation of five compounds from leaves of<i>Andrographis paniculata</i>(Burm. f.) Nees by off-line two-dimensional high-speed counter-current chromatography combined with gradient and recycling elution" @default.
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- W2098975276 doi "https://doi.org/10.1002/jssc.201401458" @default.
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