FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2098982921> ?p ?o ?g. }

• W2098982921 endingPage "64" @default.
• W2098982921 startingPage "55" @default.
• W2098982921 abstract "Clostridium difficile, a human pathogen, produces two very large protein toxins, A and B (250-600 kDa), which resist dissociation into subunits. To clone the toxin A gene, a genomic library of 3-8 kb chromosomal DNA fragments of C. difficile strain VPI 10463 established in pUC12 was screened with a rabbit polyclonal toxin A antiserum. Thirty-five clones were isolated which carried 2.5-7.0 kb inserts representing a 10 kb region of the C. difficile genome. All the inserts were oriented in the same direction, suggesting that toxin A gene expression was under control of the lac promoter of the pUC12 vector. Western blot experiments revealed the presence of low amounts of fusion proteins of variable size (30-170 kDa) in Escherichia coli strains harbouring recombinant plasmids. As deduced from subcloning experiments, the DNA sequences encoding toxin A comprised about 4 kb, corresponding to about 140 kDa of the 300-600 kDa protein. This was either due to incomplete cloning of the gene or it might indicate a subunit composition of toxin A. No additional gene(s) with homology to the cloned toxin A gene was detected." @default.
• W2098982921 created "2016-06-24" @default.
• W2098982921 creator A5001439152 @default.
• W2098982921 creator A5064243203 @default.
• W2098982921 creator A5079963261 @default.
• W2098982921 creator A5085441376 @default.
• W2098982921 date "1989-01-01" @default.
• W2098982921 modified "2023-10-01" @default.
• W2098982921 title "Cloning and Characterization of Overlapping DNA Fragments of the Toxin A Gene of Clostridium difficile" @default.
• W2098982921 cites W1501031736 @default.
• W2098982921 cites W1536037525 @default.
• W2098982921 cites W1613179247 @default.
• W2098982921 cites W17245457 @default.
• W2098982921 cites W1853771818 @default.
• W2098982921 cites W1922367055 @default.
• W2098982921 cites W1933339962 @default.
• W2098982921 cites W1942228758 @default.
• W2098982921 cites W1957453673 @default.
• W2098982921 cites W1967999707 @default.
• W2098982921 cites W2028622989 @default.
• W2098982921 cites W2063450941 @default.
• W2098982921 cites W2086940218 @default.
• W2098982921 cites W2100837269 @default.
• W2098982921 cites W2103254493 @default.
• W2098982921 cites W2111716321 @default.
• W2098982921 cites W2113881244 @default.
• W2098982921 cites W2122965320 @default.
• W2098982921 cites W2124622016 @default.
• W2098982921 cites W2132417094 @default.
• W2098982921 cites W2144634347 @default.
• W2098982921 cites W2165346253 @default.
• W2098982921 cites W2537619017 @default.
• W2098982921 doi "https://doi.org/10.1099/00221287-135-1-55" @default.
• W2098982921 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2506313" @default.
• W2098982921 hasPublicationYear "1989" @default.
• W2098982921 type Work @default.
• W2098982921 sameAs 2098982921 @default.
• W2098982921 citedByCount "11" @default.
• W2098982921 countsByYear W20989829212021 @default.
• W2098982921 crossrefType "journal-article" @default.
• W2098982921 hasAuthorship W2098982921A5001439152 @default.
• W2098982921 hasAuthorship W2098982921A5064243203 @default.
• W2098982921 hasAuthorship W2098982921A5079963261 @default.
• W2098982921 hasAuthorship W2098982921A5085441376 @default.
• W2098982921 hasBestOaLocation W20989829211 @default.
• W2098982921 hasConcept C104317684 @default.
• W2098982921 hasConcept C133307689 @default.
• W2098982921 hasConcept C13575015 @default.
• W2098982921 hasConcept C150194340 @default.
• W2098982921 hasConcept C153911025 @default.
• W2098982921 hasConcept C163005120 @default.
• W2098982921 hasConcept C167625842 @default.
• W2098982921 hasConcept C168374021 @default.
• W2098982921 hasConcept C17757408 @default.
• W2098982921 hasConcept C19924922 @default.
• W2098982921 hasConcept C22744801 @default.
• W2098982921 hasConcept C2776441376 @default.
• W2098982921 hasConcept C2777367657 @default.
• W2098982921 hasConcept C2994496256 @default.
• W2098982921 hasConcept C40767141 @default.
• W2098982921 hasConcept C48292096 @default.
• W2098982921 hasConcept C501593827 @default.
• W2098982921 hasConcept C54355233 @default.
• W2098982921 hasConcept C547475151 @default.
• W2098982921 hasConcept C86803240 @default.
• W2098982921 hasConcept C89423630 @default.
• W2098982921 hasConcept C97505648 @default.
• W2098982921 hasConceptScore W2098982921C104317684 @default.
• W2098982921 hasConceptScore W2098982921C133307689 @default.
• W2098982921 hasConceptScore W2098982921C13575015 @default.
• W2098982921 hasConceptScore W2098982921C150194340 @default.
• W2098982921 hasConceptScore W2098982921C153911025 @default.
• W2098982921 hasConceptScore W2098982921C163005120 @default.
• W2098982921 hasConceptScore W2098982921C167625842 @default.
• W2098982921 hasConceptScore W2098982921C168374021 @default.
• W2098982921 hasConceptScore W2098982921C17757408 @default.
• W2098982921 hasConceptScore W2098982921C19924922 @default.
• W2098982921 hasConceptScore W2098982921C22744801 @default.
• W2098982921 hasConceptScore W2098982921C2776441376 @default.
• W2098982921 hasConceptScore W2098982921C2777367657 @default.
• W2098982921 hasConceptScore W2098982921C2994496256 @default.
• W2098982921 hasConceptScore W2098982921C40767141 @default.
• W2098982921 hasConceptScore W2098982921C48292096 @default.
• W2098982921 hasConceptScore W2098982921C501593827 @default.
• W2098982921 hasConceptScore W2098982921C54355233 @default.
• W2098982921 hasConceptScore W2098982921C547475151 @default.
• W2098982921 hasConceptScore W2098982921C86803240 @default.
• W2098982921 hasConceptScore W2098982921C89423630 @default.
• W2098982921 hasConceptScore W2098982921C97505648 @default.
• W2098982921 hasIssue "1" @default.
• W2098982921 hasLocation W20989829211 @default.
• W2098982921 hasLocation W20989829212 @default.
• W2098982921 hasOpenAccess W2098982921 @default.
• W2098982921 hasPrimaryLocation W20989829211 @default.
• W2098982921 hasRelatedWork W1917998177 @default.
• W2098982921 hasRelatedWork W2003283696 @default.
• W2098982921 hasRelatedWork W2009728107 @default.
• W2098982921 hasRelatedWork W2055427449 @default.

• next