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• W209900511 abstract "Thirteen phage clones containing low-copy sequences were isolated from a human DNA library and tested for their ability to detect restriction fragment length polymorphisms (RFLPs). Reported are the RFLPs revealed with each clone, all found in frequencies useful for linkage studies. Cytological data are available for five of the 13 clones, with regional assignments made for three of the markers by in situ hybridization. It is concluded that phage clones containing large unique DNA inserts detect multiple RFLPs with high efficiency. An analysis of the relative efficiency of 20 restriction enzymes for detecting single nucleotide changes is discussed by comparing the observed data to those expected on the basis of recognition and potential site frequencies, as computed from the dinucleotide distribution. Finally, in an effort to facilitate linkage studies using polymorphic DNA sequences, experiments were made with pools of probes from various sources." @default.
• W209900511 created "2016-06-24" @default.
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• W209900511 date "1985-07-01" @default.
• W209900511 modified "2023-09-27" @default.
• W209900511 title "A systematic approach for detecting high-frequency restriction fragment length polymorphisms using large genomic probes." @default.
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• W209900511 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1684621" @default.
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