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• W2099080339 abstract "ABSTRACT At the onset of endospore formation in Bacillus subtilis , the DNA binding protein Spo0A activates transcription from two types of promoters. The first type includes the spoIIG and spoIIE promoters, which are used by σ A -RNA polymerase, whereas the second type includes the spoIIA promoter, which is used by RNA polymerase containing the secondary sigma factor σ H . Previous genetic analyses have identified specific amino acids in α-helix E of Spo0A that are important for activation of Spo0A-dependent, σ A -dependent promoters. However, these amino acids are not required for activation of the σ H -dependent spoIIA promoter. We now report the effects of additional single-amino-acid substitutions and the effects of deletions in α-helix E. The effects of alanine substitutions revealed one new position (239) in Spo0A that appears to be specifically required for activation of the σ A -dependent promoters. Based on the effects of a deletion mutation, we suggest that α-helix E in Spo0A is not directly involved in interaction with σ H -RNA polymerase." @default.
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• W2099080339 date "2004-02-15" @default.
• W2099080339 modified "2023-10-10" @default.
• W2099080339 title "α-Helix E of Spo0A Is Required for σ ^A - but Not for σ ^H -Dependent Promoter Activation in <i>Bacillus subtilis</i>" @default.
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• W2099080339 doi "https://doi.org/10.1128/jb.186.4.1078-1083.2004" @default.
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