FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2099185954> ?p ?o ?g. }

• W2099185954 endingPage "4029" @default.
• W2099185954 startingPage "4021" @default.
• W2099185954 abstract "ABSTRACT Autoinducer 2 (AI-2) produced by the oral pathogen Actinobacillus actinomycetemcomitans influences growth of the organism under iron limitation and regulates the expression of iron uptake genes. However, the cellular components that mediate the response of A. actinomycetemcomitans to AI-2 have not been fully characterized. Analysis of the complete genome sequence of A. actinomycetemcomitans ( www.oralgen.lanl.gov ) indicated that the RbsB protein was related to LuxP, the AI-2 receptor of Vibrio harveyi . To determine if RbsB interacts with AI-2, the bioluminescence of the reporter strain V. harveyi BB170 (sensor 1−, sensor 2+) was determined after stimulation with partially purified AI-2 from A. actinomycetemcomitans or conditioned medium from V. harveyi cultures in the presence and absence of purified six-His-tagged RbsB. RbsB efficiently inhibited V. harveyi bioluminescence induced by both A. actinomycetemcomitans AI-2 and V. harveyi AI-2 in a dose-dependent manner, suggesting that RbsB competes with LuxP for AI-2. Fifty percent inhibition occurred with approximately 0.3 nM RbsB for A. actinomycetemcomitans AI-2 and 15 nM RbsB for V. harveyi AI-2. RbsB-mediated inhibition of V. harveyi bioluminescence was reversed by the addition of 50 mM ribose, suggesting that A. actinomycetemcomitans AI-2 and ribose bind at the same site of RbsB. The RbsB/AI-2 complex was thermostable since A. actinomycetemcomitans AI-2 could not be recovered by heating. This was not due to heat inactivation of A. actinomycetemcomitans AI-2 since signal activity was unaffected by heating in the absence of RbsB. Furthermore, an isogenic A. actinomycetemcomitans mutant that was unable to express rbsB was deficient in depleting A. actinomycetemcomitans AI-2 from solution relative to the wild-type organism. Inactivation of rbsB also influenced the ability of the organism to grow under iron-limiting conditions. The mutant strain attained a cell density of approximately 30% that of the wild-type organism under iron limitation. In addition, real-time PCR showed that the expression of afuABC , encoding a major ferric ion transporter, was reduced by approximately eightfold in the rbsB mutant. This phenotype was similar to that of a LuxS-deficient mutant of A. actinomycetemcomitans that is unable to produce AI-2. Together, our results suggest that RbsB may play a role in the response of A. actinomycetemcomitans to AI-2." @default.
• W2099185954 created "2016-06-24" @default.
• W2099185954 creator A5006760859 @default.
• W2099185954 creator A5008946134 @default.
• W2099185954 creator A5009202130 @default.
• W2099185954 creator A5085761568 @default.
• W2099185954 date "2006-07-01" @default.
• W2099185954 modified "2023-10-07" @default.
• W2099185954 title "The <i>Actinobacillus actinomycetemcomitans</i> Ribose Binding Protein RbsB Interacts with Cognate and Heterologous Autoinducer 2 Signals" @default.
• W2099185954 cites W1505015935 @default.
• W2099185954 cites W1851276803 @default.
• W2099185954 cites W1902899140 @default.
• W2099185954 cites W1927570869 @default.
• W2099185954 cites W1959548112 @default.
• W2099185954 cites W1972435034 @default.
• W2099185954 cites W1991095439 @default.
• W2099185954 cites W1993616876 @default.
• W2099185954 cites W1994462493 @default.
• W2099185954 cites W1995186414 @default.
• W2099185954 cites W1996872292 @default.
• W2099185954 cites W2040641257 @default.
• W2099185954 cites W2045154470 @default.
• W2099185954 cites W2052255348 @default.
• W2099185954 cites W2055873811 @default.
• W2099185954 cites W2056932987 @default.
• W2099185954 cites W2060779329 @default.
• W2099185954 cites W2064612941 @default.
• W2099185954 cites W2071784720 @default.
• W2099185954 cites W2082410833 @default.
• W2099185954 cites W2098064749 @default.
• W2099185954 cites W2098626852 @default.
• W2099185954 cites W2103359264 @default.
• W2099185954 cites W2109277022 @default.
• W2099185954 cites W2112063053 @default.
• W2099185954 cites W2115808190 @default.
• W2099185954 cites W2120960183 @default.
• W2099185954 cites W2121992777 @default.
• W2099185954 cites W2134555745 @default.
• W2099185954 cites W2144640625 @default.
• W2099185954 cites W2145166342 @default.
• W2099185954 cites W2150890529 @default.
• W2099185954 cites W2151001863 @default.
• W2099185954 cites W2154819662 @default.
• W2099185954 cites W2157092031 @default.
• W2099185954 cites W2158326203 @default.
• W2099185954 cites W2158792388 @default.
• W2099185954 cites W2172030218 @default.
• W2099185954 cites W2326717311 @default.
• W2099185954 cites W2341183439 @default.
• W2099185954 cites W4211005858 @default.
• W2099185954 doi "https://doi.org/10.1128/iai.01741-05" @default.
• W2099185954 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1489740" @default.
• W2099185954 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16790775" @default.
• W2099185954 hasPublicationYear "2006" @default.
• W2099185954 type Work @default.
• W2099185954 sameAs 2099185954 @default.
• W2099185954 citedByCount "78" @default.
• W2099185954 countsByYear W20991859542012 @default.
• W2099185954 countsByYear W20991859542013 @default.
• W2099185954 countsByYear W20991859542014 @default.
• W2099185954 countsByYear W20991859542015 @default.
• W2099185954 countsByYear W20991859542016 @default.
• W2099185954 countsByYear W20991859542017 @default.
• W2099185954 countsByYear W20991859542018 @default.
• W2099185954 countsByYear W20991859542019 @default.
• W2099185954 countsByYear W20991859542020 @default.
• W2099185954 countsByYear W20991859542021 @default.
• W2099185954 countsByYear W20991859542022 @default.
• W2099185954 countsByYear W20991859542023 @default.
• W2099185954 crossrefType "journal-article" @default.
• W2099185954 hasAuthorship W2099185954A5006760859 @default.
• W2099185954 hasAuthorship W2099185954A5008946134 @default.
• W2099185954 hasAuthorship W2099185954A5009202130 @default.
• W2099185954 hasAuthorship W2099185954A5085761568 @default.
• W2099185954 hasBestOaLocation W20991859542 @default.
• W2099185954 hasConcept C104317684 @default.
• W2099185954 hasConcept C111335760 @default.
• W2099185954 hasConcept C153911025 @default.
• W2099185954 hasConcept C2776154503 @default.
• W2099185954 hasConcept C2780115692 @default.
• W2099185954 hasConcept C2780728835 @default.
• W2099185954 hasConcept C2781275326 @default.
• W2099185954 hasConcept C523546767 @default.
• W2099185954 hasConcept C54009773 @default.
• W2099185954 hasConcept C54355233 @default.
• W2099185954 hasConcept C55493867 @default.
• W2099185954 hasConcept C86803240 @default.
• W2099185954 hasConcept C89423630 @default.
• W2099185954 hasConceptScore W2099185954C104317684 @default.
• W2099185954 hasConceptScore W2099185954C111335760 @default.
• W2099185954 hasConceptScore W2099185954C153911025 @default.
• W2099185954 hasConceptScore W2099185954C2776154503 @default.
• W2099185954 hasConceptScore W2099185954C2780115692 @default.
• W2099185954 hasConceptScore W2099185954C2780728835 @default.
• W2099185954 hasConceptScore W2099185954C2781275326 @default.
• W2099185954 hasConceptScore W2099185954C523546767 @default.
• W2099185954 hasConceptScore W2099185954C54009773 @default.
• W2099185954 hasConceptScore W2099185954C54355233 @default.

• next