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- W2099187812 abstract "It is well established that when E. coli 30S ribosomal subunits are irradiated with ultraviolet light under mild conditions a specific cross-link is formed between protein S7 and the 16S RNA. Methodology is presented for the analysis of the single nucleotide residue concerned in this cross-link. Firstly, the identity of the ribonuclease T1 octanucleotide attached to S7 is confirmed by a new method, which involves isolation and analysis of S7-polynucleotide complexes containing 30 -- 40 nucleotides. Secondly, the isolated S7-octanucleotide complex is digested successively with ribonuclease A, proteinase K and ribonuclease T2, and the nucleotides liberated are identified. The results show unambiguously that uridine residue number 1239 in the 16S RNA sequence is cross-linked to protein S7." @default.
- W2099187812 created "2016-06-24" @default.
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- W2099187812 title "RNA-protein cross-linking in Escherichia coli 30s ribosomal subunits: precise localisation of the nucleotide in 16s RNA which is coupled to protein S7 by ultraviolet irradiation" @default.
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- W2099187812 doi "https://doi.org/10.1093/nar/6.5.1775" @default.
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