Matches in SemOpenAlex for { <https://semopenalex.org/work/W2099197457> ?p ?o ?g. }
- W2099197457 abstract "AbstractSucrose phosphorylase is a bacterial α-transglucosidase that catalyses glucosyl transfer from sucrose to phosphate, releasing d-fructose and α-d-glucose 1-phosphate as the product of the first (enzyme glucosylation) and second (enzyme deglucosylation) step of the enzymatic reaction, respectively. The transferred glucosyl moiety of sucrose is accommodated at the catalytic subsite of the phosphorylase through a network of charged hydrogen bonds whereby a highly conserved residue pair of Asp and Arg points towards the equatorial hydroxyl at C4. To examine the role of this ‘hyperpolar’ binding site for the substrate 4-OH, we have mutated Asp49 and Arg395 of Leuconostoc mesenteroides sucrose phosphorylase individually to Ala (D49A) and Leu (R395L), respectively, and also prepared an ‘uncharged’ double mutant harbouring both site-directed substitutions. The efficiency for enzyme glucosylation from sucrose was massively decreased in purified preparations of D49A (107-fold) and R395L (105-fold) as compare..." @default.
- W2099197457 created "2016-06-24" @default.
- W2099197457 creator A5017795673 @default.
- W2099197457 creator A5051203357 @default.
- W2099197457 creator A5089993099 @default.
- W2099197457 date "2012-05-01" @default.
- W2099197457 modified "2023-10-01" @default.
- W2099197457 title "Probing enzyme–substrate interactions at the catalytic subsite ofLeuconostoc mesenteroidessucrose phosphorylase with site-directed mutagenesis: the roles of Asp49and Arg395" @default.
- W2099197457 cites W137703748 @default.
- W2099197457 cites W1513388612 @default.
- W2099197457 cites W1590476964 @default.
- W2099197457 cites W1963553814 @default.
- W2099197457 cites W1967539420 @default.
- W2099197457 cites W1973119179 @default.
- W2099197457 cites W1973608763 @default.
- W2099197457 cites W1980231050 @default.
- W2099197457 cites W1981867690 @default.
- W2099197457 cites W1982214757 @default.
- W2099197457 cites W1993634515 @default.
- W2099197457 cites W1996699780 @default.
- W2099197457 cites W1997267426 @default.
- W2099197457 cites W1999898395 @default.
- W2099197457 cites W2007938336 @default.
- W2099197457 cites W2011467657 @default.
- W2099197457 cites W2011744948 @default.
- W2099197457 cites W2019165327 @default.
- W2099197457 cites W2019697474 @default.
- W2099197457 cites W2028253641 @default.
- W2099197457 cites W2029674059 @default.
- W2099197457 cites W2041478739 @default.
- W2099197457 cites W2052745028 @default.
- W2099197457 cites W2053459584 @default.
- W2099197457 cites W2054780477 @default.
- W2099197457 cites W2062539123 @default.
- W2099197457 cites W2065609475 @default.
- W2099197457 cites W2069420027 @default.
- W2099197457 cites W2073918827 @default.
- W2099197457 cites W2090080358 @default.
- W2099197457 cites W2092002806 @default.
- W2099197457 cites W2095016491 @default.
- W2099197457 cites W2103083463 @default.
- W2099197457 cites W2110958200 @default.
- W2099197457 cites W2115630143 @default.
- W2099197457 cites W2122631536 @default.
- W2099197457 cites W2123719065 @default.
- W2099197457 cites W2127670645 @default.
- W2099197457 cites W2136505424 @default.
- W2099197457 cites W2139052360 @default.
- W2099197457 cites W2143205121 @default.
- W2099197457 cites W2155468472 @default.
- W2099197457 cites W2261198396 @default.
- W2099197457 cites W2418361432 @default.
- W2099197457 doi "https://doi.org/10.3109/10242422.2012.674720" @default.
- W2099197457 hasPublicationYear "2012" @default.
- W2099197457 type Work @default.
- W2099197457 sameAs 2099197457 @default.
- W2099197457 citedByCount "5" @default.
- W2099197457 countsByYear W20991974572015 @default.
- W2099197457 countsByYear W20991974572019 @default.
- W2099197457 countsByYear W20991974572020 @default.
- W2099197457 countsByYear W20991974572021 @default.
- W2099197457 crossrefType "journal-article" @default.
- W2099197457 hasAuthorship W2099197457A5017795673 @default.
- W2099197457 hasAuthorship W2099197457A5051203357 @default.
- W2099197457 hasAuthorship W2099197457A5089993099 @default.
- W2099197457 hasConcept C103408237 @default.
- W2099197457 hasConcept C104317684 @default.
- W2099197457 hasConcept C111368507 @default.
- W2099197457 hasConcept C127313418 @default.
- W2099197457 hasConcept C143065580 @default.
- W2099197457 hasConcept C16318435 @default.
- W2099197457 hasConcept C181199279 @default.
- W2099197457 hasConcept C185592680 @default.
- W2099197457 hasConcept C196032511 @default.
- W2099197457 hasConcept C204779464 @default.
- W2099197457 hasConcept C2775920511 @default.
- W2099197457 hasConcept C2776970464 @default.
- W2099197457 hasConcept C2777289219 @default.
- W2099197457 hasConcept C2777423746 @default.
- W2099197457 hasConcept C2777957631 @default.
- W2099197457 hasConcept C2778597767 @default.
- W2099197457 hasConcept C41183919 @default.
- W2099197457 hasConcept C523546767 @default.
- W2099197457 hasConcept C54355233 @default.
- W2099197457 hasConcept C55493867 @default.
- W2099197457 hasConcept C69118441 @default.
- W2099197457 hasConcept C71240020 @default.
- W2099197457 hasConcept C86803240 @default.
- W2099197457 hasConceptScore W2099197457C103408237 @default.
- W2099197457 hasConceptScore W2099197457C104317684 @default.
- W2099197457 hasConceptScore W2099197457C111368507 @default.
- W2099197457 hasConceptScore W2099197457C127313418 @default.
- W2099197457 hasConceptScore W2099197457C143065580 @default.
- W2099197457 hasConceptScore W2099197457C16318435 @default.
- W2099197457 hasConceptScore W2099197457C181199279 @default.
- W2099197457 hasConceptScore W2099197457C185592680 @default.
- W2099197457 hasConceptScore W2099197457C196032511 @default.
- W2099197457 hasConceptScore W2099197457C204779464 @default.
- W2099197457 hasConceptScore W2099197457C2775920511 @default.
- W2099197457 hasConceptScore W2099197457C2776970464 @default.