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- W2099204510 abstract "Two src family kinases, lck and fyn, participate in the activation of T lymphocytes. Both of these protein tyrosine kinases are thought to function via their interaction with cell surface receptors. Thus, lck is associated with CD4, CD8, and Thy-1, whereas fyn is associated with the T cell antigen receptor and Thy-1. In this study, the intracellular localization of these two protein tyrosine kinases in T cells was analyzed by immunofluorescence and confocal microscopy. Lck was present at the plasma membrane, consistent with its proposed role in transmembrane signalling, and was also associated with pericentrosomal vesicles which co-localized with the cation-independent mannose 6-phosphate receptor. Surprisingly, fyn was not detected at the plasma membrane in either Jurkat T cells or T lymphoblasts but was closely associated with the centrosome and to microtubule bundles radiating from the centrosome. In mitotic cells, fyn co-localized with the mitotic spindle and poles. The essentially non-overlapping intracellular distributions of lck and fyn suggest that these kinases may be accessible to distinct regulatory proteins and substrates and, therefore, may regulate different aspects of T cell activation. Anti-phosphotyrosine antibody staining at the plasma membrane increases dramatically after CD3 cross-linking of Jurkat T cells. The localization of lck to the plasma membrane suggests that it may participate in mediating this increase in tyrosine phosphorylation, rather than fyn. Furthermore, the distribution of fyn in mitotic cells raises the possibility that it functions at the M phase of the cell cycle." @default.
- W2099204510 created "2016-06-24" @default.
- W2099204510 creator A5031817650 @default.
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- W2099204510 creator A5064583816 @default.
- W2099204510 creator A5079491369 @default.
- W2099204510 date "1994-05-01" @default.
- W2099204510 modified "2023-09-30" @default.
- W2099204510 title "Distinct intracellular localization of Lck and Fyn protein tyrosine kinases in human T lymphocytes." @default.
- W2099204510 cites W1516065974 @default.
- W2099204510 cites W1529387078 @default.
- W2099204510 cites W1539361761 @default.
- W2099204510 cites W1561243612 @default.
- W2099204510 cites W1561731791 @default.
- W2099204510 cites W1578244808 @default.
- W2099204510 cites W1581148997 @default.
- W2099204510 cites W1602215669 @default.
- W2099204510 cites W1602468041 @default.
- W2099204510 cites W168147921 @default.
- W2099204510 cites W1897328392 @default.
- W2099204510 cites W1964774404 @default.
- W2099204510 cites W1972825826 @default.
- W2099204510 cites W1972974591 @default.
- W2099204510 cites W1974517994 @default.
- W2099204510 cites W1975791322 @default.
- W2099204510 cites W1977984433 @default.
- W2099204510 cites W1980554628 @default.
- W2099204510 cites W1990693463 @default.
- W2099204510 cites W1991982062 @default.
- W2099204510 cites W1993572893 @default.
- W2099204510 cites W1994514513 @default.
- W2099204510 cites W1994772282 @default.
- W2099204510 cites W1996405135 @default.
- W2099204510 cites W2000939282 @default.
- W2099204510 cites W2009196480 @default.
- W2099204510 cites W2016894006 @default.
- W2099204510 cites W2017516492 @default.
- W2099204510 cites W2023271648 @default.
- W2099204510 cites W2024083409 @default.
- W2099204510 cites W2025081899 @default.
- W2099204510 cites W2028669354 @default.
- W2099204510 cites W2032468105 @default.
- W2099204510 cites W2033306271 @default.
- W2099204510 cites W2035562653 @default.
- W2099204510 cites W2035686808 @default.
- W2099204510 cites W2037466012 @default.
- W2099204510 cites W2041875613 @default.
- W2099204510 cites W2042248645 @default.
- W2099204510 cites W2045819374 @default.
- W2099204510 cites W2047214302 @default.
- W2099204510 cites W2047945116 @default.
- W2099204510 cites W2049355608 @default.
- W2099204510 cites W2052561585 @default.
- W2099204510 cites W2057653339 @default.
- W2099204510 cites W2058885870 @default.
- W2099204510 cites W2063107082 @default.
- W2099204510 cites W2068803746 @default.
- W2099204510 cites W2077317888 @default.
- W2099204510 cites W208343037 @default.
- W2099204510 cites W2087656195 @default.
- W2099204510 cites W2090821655 @default.
- W2099204510 cites W2092732198 @default.
- W2099204510 cites W2094126922 @default.
- W2099204510 cites W2109202813 @default.
- W2099204510 cites W2129030094 @default.
- W2099204510 cites W2133503825 @default.
- W2099204510 cites W2134352843 @default.
- W2099204510 cites W2142685018 @default.
- W2099204510 cites W2148330408 @default.
- W2099204510 cites W2149087572 @default.
- W2099204510 cites W2151151593 @default.
- W2099204510 cites W2157342751 @default.
- W2099204510 cites W2157953677 @default.
- W2099204510 cites W2159431877 @default.
- W2099204510 cites W2160416978 @default.
- W2099204510 cites W2161314348 @default.
- W2099204510 cites W2163995402 @default.
- W2099204510 cites W2166101366 @default.
- W2099204510 cites W2167625024 @default.
- W2099204510 cites W2168870378 @default.
- W2099204510 cites W2174557896 @default.
- W2099204510 cites W2310647335 @default.
- W2099204510 cites W2337001455 @default.
- W2099204510 cites W2414153201 @default.
- W2099204510 cites W2467593455 @default.
- W2099204510 cites W87002874 @default.
- W2099204510 cites W90048484 @default.
- W2099204510 cites W91142790 @default.
- W2099204510 doi "https://doi.org/10.1083/jcb.125.3.639" @default.
- W2099204510 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2119993" @default.
- W2099204510 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7513706" @default.
- W2099204510 hasPublicationYear "1994" @default.
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