FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2099248086> ?p ?o ?g. }

• W2099248086 endingPage "F166" @default.
• W2099248086 startingPage "F157" @default.
• W2099248086 abstract "The mammalian kidney isoform of the essential chloride-bicarbonate exchanger AE1 differs from its erythrocyte counterpart, being shorter at its N terminus. It has previously been reported that the glycolytic enzyme GAPDH interacts only with erythrocyte AE1, by binding to the portion not found in the kidney isoform. (Chu H, Low PS. Biochem J 400:143-151, 2006). We have identified GAPDH as a candidate binding partner for the C terminus of both AE1 and AE2. We show that full-length AE1 and GAPDH coimmunoprecipitated from both human and rat kidney as well as from Madin-Darby canine kidney (MDCK) cells stably expressing kidney AE1, while in human liver, AE2 coprecipitated with GAPDH. ELISA and glutathione S-transferase (GST) pull-down assays using GST-tagged C-terminal AE1 fusion protein confirmed that the interaction is direct; fluorescence titration revealed saturable binding kinetics with Kd 2.3±0.2 μM. Further GST precipitation assays demonstrated that the D902EY residues in the D902EYDE motif located within the C terminus of AE1 are important for GAPDH binding. In vitro GAPDH activity was unaffected by C-terminal AE1 binding, unlike in erythrocytes. Also, differently from red cell N-terminal binding, GAPDH-AE1 C-terminal binding was not disrupted by phosphorylation of AE1 in kidney AE1-expressing MDCK cells. Importantly, small interfering RNA knockdown of GAPDH in these cells resulted in significant intracellular retention of AE1, with a concomitant reduction in AE1 at the cell membrane. These results indicate differences between kidney and erythrocyte AE1/GAPDH behavior and show that in the kidney, GAPDH is required for kidney AE1 to achieve stable basolateral residency." @default.
• W2099248086 created "2016-06-24" @default.
• W2099248086 creator A5000754271 @default.
• W2099248086 creator A5021117584 @default.
• W2099248086 creator A5026611358 @default.
• W2099248086 creator A5026763709 @default.
• W2099248086 creator A5034892349 @default.
• W2099248086 creator A5060662946 @default.
• W2099248086 creator A5069213420 @default.
• W2099248086 creator A5071518367 @default.
• W2099248086 creator A5084605677 @default.
• W2099248086 creator A5086235740 @default.
• W2099248086 date "2011-01-01" @default.
• W2099248086 modified "2023-09-30" @default.
• W2099248086 title "Glyceraldehyde 3-phosphate dehydrogenase is required for band 3 (anion exchanger 1) membrane residency in the mammalian kidney" @default.
• W2099248086 cites W1508076157 @default.
• W2099248086 cites W1554532255 @default.
• W2099248086 cites W1555778502 @default.
• W2099248086 cites W1561589021 @default.
• W2099248086 cites W1610051602 @default.
• W2099248086 cites W1661137134 @default.
• W2099248086 cites W1876326576 @default.
• W2099248086 cites W1934148052 @default.
• W2099248086 cites W1964299129 @default.
• W2099248086 cites W1979473634 @default.
• W2099248086 cites W1988203656 @default.
• W2099248086 cites W1988615788 @default.
• W2099248086 cites W1995111646 @default.
• W2099248086 cites W2015631023 @default.
• W2099248086 cites W2017960724 @default.
• W2099248086 cites W2021868083 @default.
• W2099248086 cites W2022787880 @default.
• W2099248086 cites W2026138425 @default.
• W2099248086 cites W2027307541 @default.
• W2099248086 cites W2038581366 @default.
• W2099248086 cites W2051588469 @default.
• W2099248086 cites W2053359084 @default.
• W2099248086 cites W2067682684 @default.
• W2099248086 cites W2070397394 @default.
• W2099248086 cites W2072338906 @default.
• W2099248086 cites W2073927199 @default.
• W2099248086 cites W2076924960 @default.
• W2099248086 cites W2084146928 @default.
• W2099248086 cites W2087821761 @default.
• W2099248086 cites W2089203011 @default.
• W2099248086 cites W2094011266 @default.
• W2099248086 cites W2107678837 @default.
• W2099248086 cites W2108231676 @default.
• W2099248086 cites W2111395938 @default.
• W2099248086 cites W2112150224 @default.
• W2099248086 cites W2118044204 @default.
• W2099248086 cites W2123463281 @default.
• W2099248086 cites W2128025298 @default.
• W2099248086 cites W2143602939 @default.
• W2099248086 cites W2157561719 @default.
• W2099248086 cites W2299294120 @default.
• W2099248086 cites W2338398300 @default.
• W2099248086 cites W2404097283 @default.
• W2099248086 cites W2410892318 @default.
• W2099248086 cites W776803945 @default.
• W2099248086 cites W966755600 @default.
• W2099248086 doi "https://doi.org/10.1152/ajprenal.00228.2010" @default.
• W2099248086 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3023227" @default.
• W2099248086 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20980406" @default.
• W2099248086 hasPublicationYear "2011" @default.
• W2099248086 type Work @default.
• W2099248086 sameAs 2099248086 @default.
• W2099248086 citedByCount "26" @default.
• W2099248086 countsByYear W20992480862012 @default.
• W2099248086 countsByYear W20992480862013 @default.
• W2099248086 countsByYear W20992480862014 @default.
• W2099248086 countsByYear W20992480862015 @default.
• W2099248086 countsByYear W20992480862016 @default.
• W2099248086 countsByYear W20992480862017 @default.
• W2099248086 countsByYear W20992480862019 @default.
• W2099248086 countsByYear W20992480862020 @default.
• W2099248086 countsByYear W20992480862021 @default.
• W2099248086 countsByYear W20992480862022 @default.
• W2099248086 crossrefType "journal-article" @default.
• W2099248086 hasAuthorship W2099248086A5000754271 @default.
• W2099248086 hasAuthorship W2099248086A5021117584 @default.
• W2099248086 hasAuthorship W2099248086A5026611358 @default.
• W2099248086 hasAuthorship W2099248086A5026763709 @default.
• W2099248086 hasAuthorship W2099248086A5034892349 @default.
• W2099248086 hasAuthorship W2099248086A5060662946 @default.
• W2099248086 hasAuthorship W2099248086A5069213420 @default.
• W2099248086 hasAuthorship W2099248086A5071518367 @default.
• W2099248086 hasAuthorship W2099248086A5084605677 @default.
• W2099248086 hasAuthorship W2099248086A5086235740 @default.
• W2099248086 hasBestOaLocation W20992480862 @default.
• W2099248086 hasConcept C134792803 @default.
• W2099248086 hasConcept C144647389 @default.
• W2099248086 hasConcept C153911025 @default.
• W2099248086 hasConcept C173396325 @default.
• W2099248086 hasConcept C181199279 @default.
• W2099248086 hasConcept C185592680 @default.
• W2099248086 hasConcept C190283241 @default.
• W2099248086 hasConcept C2776317432 @default.

• next