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- W2099255248 abstract "ABSTRACT A number of cyanobacteria from different taxonomic groups exhibited very low levels of uptake of 2-[U- 14 C]oxoglutarate. Synechococcus sp. strain PCC 7942 was transformed with DNA constructs carrying the Escherichia coli kgtP gene encoding a 2-oxoglutarate permease and a kanamycin resistance gene cassette. The Synechococcus sp. strains bearing the kgtP gene incorporated 2-oxoglutarate into the cells through an active transport process. About 75% of the radioactivity from the 2-[U- 14 C]oxoglutarate taken up that was recovered in soluble metabolites was found as glutamate and glutamine. 2-Oxoglutarate was, however, detrimental to the growth of a Synechococcus sp. strain bearing the kgtP gene." @default.
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- W2099255248 date "2000-01-01" @default.
- W2099255248 modified "2023-10-01" @default.
- W2099255248 title "Uptake of 2-Oxoglutarate in <i>Synechococcus</i> Strains Transformed with the <i>Escherichia coli kgtP</i> Gene" @default.
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- W2099255248 doi "https://doi.org/10.1128/jb.182.1.211-215.2000" @default.
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