Matches in SemOpenAlex for { <https://semopenalex.org/work/W2099292414> ?p ?o ?g. }
- W2099292414 endingPage "46" @default.
- W2099292414 startingPage "31" @default.
- W2099292414 abstract "The loss of 14C ethanolamine- and 3H choline-labelled phospholipids from rat liver during tissue preparation for electron microscopy has been examined. Column and thin-layer chromatography combined with double-label scintillation spectrometry were used to analyse the radioactive phospholipid content of the livers of rats injected simultaneously with 14C aminoethanol and 3H choline chloride. After 4 h (in vivo) the 14C and 3H labels were mainly incorporated into phosphatidyl ethanolamine and phosphatidyl choline respectively but some 14C label had been incorporated into phosphatidyl choline. Chopped rat liver was fixed in glutaraldehyde or osmium tetroxide or both sequentially and tissues were dehydrated in ethanol and embedded in Araldite. In each procedure examined the choline label proved more labile than the ethanolamine. After glutaraldehyde fixation alone complete loss of phosphatidyl choline occurred and half of the phosphatidyl ethanolamine was also lost. Following osmium tetroxide fixation negligible loss of either phosphatide occurred. In terms of phospholipid retention, no advantage was gained by glutaraldehyde fixation prior to osmium tetroxide fixation. The results show that both ethanols and embedding monomers are potent phospholipid solvents. The data also suggests that EM autoradiography of these two phosphatides may be carried out with reasonable confidence although it must be pointed out that a high degree of retention does not necessarily imply retention in situ." @default.
- W2099292414 created "2016-06-24" @default.
- W2099292414 creator A5047883451 @default.
- W2099292414 creator A5062530767 @default.
- W2099292414 date "1969-08-01" @default.
- W2099292414 modified "2023-10-13" @default.
- W2099292414 title "Quantitative studies on the preservation of choline and ethanolamine phosphatides during tissue preparation for electron microscopy" @default.
- W2099292414 cites W1522819451 @default.
- W2099292414 cites W17084386 @default.
- W2099292414 cites W1965667673 @default.
- W2099292414 cites W1968333715 @default.
- W2099292414 cites W1969410452 @default.
- W2099292414 cites W1973818220 @default.
- W2099292414 cites W1976424232 @default.
- W2099292414 cites W1989276095 @default.
- W2099292414 cites W1992500898 @default.
- W2099292414 cites W1998137801 @default.
- W2099292414 cites W1998183048 @default.
- W2099292414 cites W1998977136 @default.
- W2099292414 cites W2000425522 @default.
- W2099292414 cites W2000518806 @default.
- W2099292414 cites W2007668251 @default.
- W2099292414 cites W2024100765 @default.
- W2099292414 cites W2030702038 @default.
- W2099292414 cites W2033255315 @default.
- W2099292414 cites W2041015294 @default.
- W2099292414 cites W2042273444 @default.
- W2099292414 cites W2049703159 @default.
- W2099292414 cites W2054760402 @default.
- W2099292414 cites W2060253137 @default.
- W2099292414 cites W2062096952 @default.
- W2099292414 cites W2064030572 @default.
- W2099292414 cites W2064549064 @default.
- W2099292414 cites W2092402923 @default.
- W2099292414 cites W2095109812 @default.
- W2099292414 cites W2099292414 @default.
- W2099292414 cites W2115163939 @default.
- W2099292414 cites W2127911529 @default.
- W2099292414 cites W2141576462 @default.
- W2099292414 cites W2160846172 @default.
- W2099292414 cites W2168526937 @default.
- W2099292414 cites W2329649355 @default.
- W2099292414 cites W241733517 @default.
- W2099292414 cites W4252805780 @default.
- W2099292414 cites W571081291 @default.
- W2099292414 doi "https://doi.org/10.1111/j.1365-2818.1969.tb00692.x" @default.
- W2099292414 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4895518" @default.
- W2099292414 hasPublicationYear "1969" @default.
- W2099292414 type Work @default.
- W2099292414 sameAs 2099292414 @default.
- W2099292414 citedByCount "55" @default.
- W2099292414 countsByYear W20992924142014 @default.
- W2099292414 countsByYear W20992924142015 @default.
- W2099292414 countsByYear W20992924142017 @default.
- W2099292414 countsByYear W20992924142018 @default.
- W2099292414 countsByYear W20992924142021 @default.
- W2099292414 countsByYear W20992924142022 @default.
- W2099292414 countsByYear W20992924142023 @default.
- W2099292414 crossrefType "journal-article" @default.
- W2099292414 hasAuthorship W2099292414A5047883451 @default.
- W2099292414 hasAuthorship W2099292414A5062530767 @default.
- W2099292414 hasConcept C104317684 @default.
- W2099292414 hasConcept C120665830 @default.
- W2099292414 hasConcept C121332964 @default.
- W2099292414 hasConcept C146249460 @default.
- W2099292414 hasConcept C185592680 @default.
- W2099292414 hasConcept C2777529624 @default.
- W2099292414 hasConcept C2778918659 @default.
- W2099292414 hasConcept C2779216040 @default.
- W2099292414 hasConcept C2779424567 @default.
- W2099292414 hasConcept C2781123555 @default.
- W2099292414 hasConcept C41625074 @default.
- W2099292414 hasConcept C43617362 @default.
- W2099292414 hasConcept C55493867 @default.
- W2099292414 hasConcept C93877712 @default.
- W2099292414 hasConceptScore W2099292414C104317684 @default.
- W2099292414 hasConceptScore W2099292414C120665830 @default.
- W2099292414 hasConceptScore W2099292414C121332964 @default.
- W2099292414 hasConceptScore W2099292414C146249460 @default.
- W2099292414 hasConceptScore W2099292414C185592680 @default.
- W2099292414 hasConceptScore W2099292414C2777529624 @default.
- W2099292414 hasConceptScore W2099292414C2778918659 @default.
- W2099292414 hasConceptScore W2099292414C2779216040 @default.
- W2099292414 hasConceptScore W2099292414C2779424567 @default.
- W2099292414 hasConceptScore W2099292414C2781123555 @default.
- W2099292414 hasConceptScore W2099292414C41625074 @default.
- W2099292414 hasConceptScore W2099292414C43617362 @default.
- W2099292414 hasConceptScore W2099292414C55493867 @default.
- W2099292414 hasConceptScore W2099292414C93877712 @default.
- W2099292414 hasIssue "1" @default.
- W2099292414 hasLocation W20992924141 @default.
- W2099292414 hasLocation W20992924142 @default.
- W2099292414 hasOpenAccess W2099292414 @default.
- W2099292414 hasPrimaryLocation W20992924141 @default.
- W2099292414 hasRelatedWork W1514597216 @default.
- W2099292414 hasRelatedWork W1992053599 @default.
- W2099292414 hasRelatedWork W2019250733 @default.
- W2099292414 hasRelatedWork W2041476721 @default.