FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2099293414> ?p ?o ?g. }

• W2099293414 abstract "Abstract Background MicroRNAs (miRNAs), a ubiquitous class of short RNAs, play vital roles in physiological and biochemical processes in plants by mediating gene silencing at post-transcriptional (PTGS) level. Tomato is a model system to study molecular basis of fleshy fruit ripening and senescence, ethylene biosynthesis and signal transduction owing to its genetic and molecular tractability. To study the functions of miRNAs in tomato fruit ripening and senescence, and their possible roles in ethylene response, the next generation sequencing method was employed to identify miRNAs in tomato fruit. Bioinformatics and molecular biology approaches were combined to profile the miRNAs expression patterns at three different fruit ripening stages and by exogenous ethylene treatment. Results In addition to 7 novel miRNA families, 103 conserved miRNAs belonging to 24 families and 10 non-conserved miRNAs matching 9 families were identified in our libraries. The targets of many these miRNAs were predicted to be transcriptional factors. Other targets are known to play roles in the regulation of metabolic processes. Interestingly, some targets were predicted to be involved in fruit ripening and softening, such as Pectate Lyase, beta-galactosidase, while a few others were predicted to be involved in ethylene biosynthesis and signaling pathway, such as ACS, EIN2 and CTR1. The expression patterns of a number of such miRNAs at three ripening stages were confirmed by stem-loop RT-PCR, which showed a strong negative correlation with that of their targets. The regulation of exogenous ethylene on miRNAs expression profiles were analyzed simultaneously, and 3 down-regulated, 5 up-regulated miRNAs were found in this study. Conclusions A combination of high throughput sequencing and molecular biology approaches was used to explore the involvement of miRNAs during fruit ripening. Several miRNAs showed differential expression profiles during fruit ripening, and a number of miRNAs were influenced by ethylene treatment. The results suggest the importance of miRNAs in fruit ripening and ethylene response." @default.
• W2099293414 created "2016-06-24" @default.
• W2099293414 creator A5003149305 @default.
• W2099293414 creator A5015655065 @default.
• W2099293414 creator A5028990220 @default.
• W2099293414 creator A5047401051 @default.
• W2099293414 creator A5061754700 @default.
• W2099293414 creator A5074630977 @default.
• W2099293414 creator A5077219469 @default.
• W2099293414 creator A5085442298 @default.
• W2099293414 creator A5085821630 @default.
• W2099293414 creator A5089614699 @default.
• W2099293414 date "2012-01-09" @default.
• W2099293414 modified "2023-10-10" @default.
• W2099293414 title "Sculpting the maturation, softening and ethylene pathway: The influences of microRNAs on tomato fruits" @default.
• W2099293414 cites W1567360163 @default.
• W2099293414 cites W1885622104 @default.
• W2099293414 cites W1964301144 @default.
• W2099293414 cites W1966612206 @default.
• W2099293414 cites W1967543716 @default.
• W2099293414 cites W1968930391 @default.
• W2099293414 cites W1969146251 @default.
• W2099293414 cites W1969172553 @default.
• W2099293414 cites W1969398747 @default.
• W2099293414 cites W1975390562 @default.
• W2099293414 cites W1986328541 @default.
• W2099293414 cites W1997413633 @default.
• W2099293414 cites W1997956266 @default.
• W2099293414 cites W2005476363 @default.
• W2099293414 cites W2006312644 @default.
• W2099293414 cites W2012361460 @default.
• W2099293414 cites W2012841698 @default.
• W2099293414 cites W2018403717 @default.
• W2099293414 cites W2020448394 @default.
• W2099293414 cites W2020878700 @default.
• W2099293414 cites W2023118437 @default.
• W2099293414 cites W2026582537 @default.
• W2099293414 cites W2029073522 @default.
• W2099293414 cites W2039096418 @default.
• W2099293414 cites W2040368735 @default.
• W2099293414 cites W2040733779 @default.
• W2099293414 cites W2042004955 @default.
• W2099293414 cites W2044201202 @default.
• W2099293414 cites W2049660411 @default.
• W2099293414 cites W2056837410 @default.
• W2099293414 cites W2064379205 @default.
• W2099293414 cites W2069015809 @default.
• W2099293414 cites W2070571588 @default.
• W2099293414 cites W2071574444 @default.
• W2099293414 cites W2071671704 @default.
• W2099293414 cites W2078627652 @default.
• W2099293414 cites W2079165446 @default.
• W2099293414 cites W2082538881 @default.
• W2099293414 cites W2087821308 @default.
• W2099293414 cites W2092002187 @default.
• W2099293414 cites W2092262391 @default.
• W2099293414 cites W2093296144 @default.
• W2099293414 cites W2094990382 @default.
• W2099293414 cites W2096224641 @default.
• W2099293414 cites W2096963672 @default.
• W2099293414 cites W2097595476 @default.
• W2099293414 cites W2099660828 @default.
• W2099293414 cites W2100932663 @default.
• W2099293414 cites W2102681779 @default.
• W2099293414 cites W2103291924 @default.
• W2099293414 cites W2104080203 @default.
• W2099293414 cites W2106268948 @default.
• W2099293414 cites W2106867028 @default.
• W2099293414 cites W2109460959 @default.
• W2099293414 cites W2109610428 @default.
• W2099293414 cites W2111046910 @default.
• W2099293414 cites W2111434558 @default.
• W2099293414 cites W2112167962 @default.
• W2099293414 cites W2114206214 @default.
• W2099293414 cites W2116166762 @default.
• W2099293414 cites W2116672276 @default.
• W2099293414 cites W2120186352 @default.
• W2099293414 cites W2121673931 @default.
• W2099293414 cites W2123079914 @default.
• W2099293414 cites W2125202677 @default.
• W2099293414 cites W2132946216 @default.
• W2099293414 cites W2133478935 @default.
• W2099293414 cites W2134995632 @default.
• W2099293414 cites W2135013982 @default.
• W2099293414 cites W2135015580 @default.
• W2099293414 cites W2137672602 @default.
• W2099293414 cites W2138026076 @default.
• W2099293414 cites W2140415861 @default.
• W2099293414 cites W2142214917 @default.
• W2099293414 cites W2142680879 @default.
• W2099293414 cites W2144818666 @default.
• W2099293414 cites W2147688972 @default.
• W2099293414 cites W2147908409 @default.
• W2099293414 cites W2148549632 @default.
• W2099293414 cites W2149199504 @default.
• W2099293414 cites W2152799036 @default.
• W2099293414 cites W2155024253 @default.
• W2099293414 cites W2156852055 @default.
• W2099293414 cites W2157542060 @default.
• W2099293414 cites W2158894404 @default.

• next