Matches in SemOpenAlex for { <https://semopenalex.org/work/W2099346871> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2099346871 endingPage "453" @default.
- W2099346871 startingPage "445" @default.
- W2099346871 abstract "Tetracycline-resistant (Tetr) mutants RAD1, RAD2, RAD6, and RAD7 were isolated from Bacillus subtilis BC92 after protoplasting, polyethylene glycol treatment, and regeneration on a medium containing tetracycline. The Tetr phenotype in RAD1, RAD2, and RAD6 was very stable with less than 5% loss of resistance after 30 generations of growth in the absence of selection. Of the four isolates, three contained amplified chromosomal DNA closely associated with the Tetr phenotype. The intensity of restriction fragments present in HindIII and EcoRI digests of chromosomal DNA from RAD1, RAD6, and RAD7 indicated the presence of tandemly duplicated DNA. Disparity in the size and number of amplified fragments suggested that the tandemly duplicated DNA is different in all three isolates. The sizes of the duplicated DNA present in RAD1, RAD6, and RAD7 were estimated to be 10, 19, and 20 kilobases, respectively. No amplified DNA was detected in RAD2. Results of transductional-mapping studies with PBS1 showed that the tetracycline resistance (tet) loci of RAD1, RAD2, and RAD6 all mapped near the origin of chromosomal replication and close to the guaA locus. Amplified DNA characteristic of RAD1 and RAD6 was cotransduced with the tet locus. Cotransfer of amplified DNA with the guaA locus or other nearby loci in the absence of tet was not observed. In every case, loss of Tetr was accompanied by loss of amplified DNA. A possible explanation for the occurrence of the amplified DNA is presented." @default.
- W2099346871 created "2016-06-24" @default.
- W2099346871 creator A5009430280 @default.
- W2099346871 creator A5019183950 @default.
- W2099346871 date "1985-08-01" @default.
- W2099346871 modified "2023-09-25" @default.
- W2099346871 title "Chromosomal-DNA amplification in Bacillus subtilis" @default.
- W2099346871 cites W1507212424 @default.
- W2099346871 cites W1548108521 @default.
- W2099346871 cites W1554059931 @default.
- W2099346871 cites W1605088984 @default.
- W2099346871 cites W1678194650 @default.
- W2099346871 cites W1800577803 @default.
- W2099346871 cites W1965743128 @default.
- W2099346871 cites W1971991212 @default.
- W2099346871 cites W1986797909 @default.
- W2099346871 cites W1989708685 @default.
- W2099346871 cites W2003399752 @default.
- W2099346871 cites W2019410656 @default.
- W2099346871 cites W2024021793 @default.
- W2099346871 cites W2041918211 @default.
- W2099346871 cites W2058705601 @default.
- W2099346871 cites W2059297512 @default.
- W2099346871 cites W2066689347 @default.
- W2099346871 cites W2107738680 @default.
- W2099346871 cites W2118744932 @default.
- W2099346871 cites W2122052317 @default.
- W2099346871 cites W2125534433 @default.
- W2099346871 cites W2135442188 @default.
- W2099346871 doi "https://doi.org/10.1128/jb.163.2.445-453.1985" @default.
- W2099346871 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/219142" @default.
- W2099346871 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2991188" @default.
- W2099346871 hasPublicationYear "1985" @default.
- W2099346871 type Work @default.
- W2099346871 sameAs 2099346871 @default.
- W2099346871 citedByCount "28" @default.
- W2099346871 countsByYear W20993468712014 @default.
- W2099346871 countsByYear W20993468712015 @default.
- W2099346871 crossrefType "journal-article" @default.
- W2099346871 hasAuthorship W2099346871A5009430280 @default.
- W2099346871 hasAuthorship W2099346871A5019183950 @default.
- W2099346871 hasBestOaLocation W20993468711 @default.
- W2099346871 hasConcept C104317684 @default.
- W2099346871 hasConcept C130123407 @default.
- W2099346871 hasConcept C153911025 @default.
- W2099346871 hasConcept C158448853 @default.
- W2099346871 hasConcept C170673058 @default.
- W2099346871 hasConcept C22744801 @default.
- W2099346871 hasConcept C2777272437 @default.
- W2099346871 hasConcept C2777274385 @default.
- W2099346871 hasConcept C523546767 @default.
- W2099346871 hasConcept C54355233 @default.
- W2099346871 hasConcept C552990157 @default.
- W2099346871 hasConcept C84289048 @default.
- W2099346871 hasConcept C84597430 @default.
- W2099346871 hasConcept C86339819 @default.
- W2099346871 hasConcept C86803240 @default.
- W2099346871 hasConceptScore W2099346871C104317684 @default.
- W2099346871 hasConceptScore W2099346871C130123407 @default.
- W2099346871 hasConceptScore W2099346871C153911025 @default.
- W2099346871 hasConceptScore W2099346871C158448853 @default.
- W2099346871 hasConceptScore W2099346871C170673058 @default.
- W2099346871 hasConceptScore W2099346871C22744801 @default.
- W2099346871 hasConceptScore W2099346871C2777272437 @default.
- W2099346871 hasConceptScore W2099346871C2777274385 @default.
- W2099346871 hasConceptScore W2099346871C523546767 @default.
- W2099346871 hasConceptScore W2099346871C54355233 @default.
- W2099346871 hasConceptScore W2099346871C552990157 @default.
- W2099346871 hasConceptScore W2099346871C84289048 @default.
- W2099346871 hasConceptScore W2099346871C84597430 @default.
- W2099346871 hasConceptScore W2099346871C86339819 @default.
- W2099346871 hasConceptScore W2099346871C86803240 @default.
- W2099346871 hasIssue "2" @default.
- W2099346871 hasLocation W20993468711 @default.
- W2099346871 hasLocation W20993468712 @default.
- W2099346871 hasLocation W20993468713 @default.
- W2099346871 hasOpenAccess W2099346871 @default.
- W2099346871 hasPrimaryLocation W20993468711 @default.
- W2099346871 hasRelatedWork W1973861642 @default.
- W2099346871 hasRelatedWork W1994044819 @default.
- W2099346871 hasRelatedWork W1996709019 @default.
- W2099346871 hasRelatedWork W1999093417 @default.
- W2099346871 hasRelatedWork W2059493961 @default.
- W2099346871 hasRelatedWork W2064949877 @default.
- W2099346871 hasRelatedWork W2360911975 @default.
- W2099346871 hasRelatedWork W2395723962 @default.
- W2099346871 hasRelatedWork W2433798839 @default.
- W2099346871 hasRelatedWork W4290367698 @default.
- W2099346871 hasVolume "163" @default.
- W2099346871 isParatext "false" @default.
- W2099346871 isRetracted "false" @default.
- W2099346871 magId "2099346871" @default.
- W2099346871 workType "article" @default.