FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2099376237> ?p ?o ?g. }

• W2099376237 endingPage "97" @default.
• W2099376237 startingPage "89" @default.
• W2099376237 abstract "Antibodies are a promising tool for the fast and selective trace detection of explosives. Unfortunately, the production of high‐quality antibodies is not trivial and often expensive. Therefore, excellent antibodies are a rare and limiting resource in fields such as biosensing, environmental analysis, diagnostics, cancer therapy, and proteomics. Here, we report the synthesis, bioconjugation, and application of the structurally optimized hapten 6‐(2,4,6‐trinitro)‐phenylhexanoic acid to improve the selectivity and sensitivity of antibodies for the detection of one of the most important explosives, trinitrotoluene. With a conjugate of bovine serum albumin and a highly purified N ‐hydroxy‐succinimide (NHS)–activated hapten, two rabbits were immunized to obtain polyclonal antibodies. The immunization process was monitored by enzyme‐linked immunosorbent assay to gain information about the progress of antibody titer and affinity. Finally, the polyclonal antibodies reached an affinity constant of (5.1 ± 0.6) × 10 9 l/mol (rabbit R1) and (2.3 ± 0.2) × 10 9 l/mol (rabbit R2). The respective assays show a minimum test midpoint (IC 50 value) of 0.1 ± 0.01 µg/l (R1) and 0.2 ± 0.02 µg/l (R2) and a working range of 0.005 to 150 µg/l (R1) and 0.007 to 200 µg/l (R2), which corresponds to more than four orders of magnitude for both. This is quite remarkable for a competitive immunoassay, which is often believed to have a narrow dynamic range. The limit of detection was calculated to 0.6 ng/l (R1) and 1.5 ng/l (R2), which is up to 100 times improvement in relation to the assay of Zeck et al. (1999) on the basis of a monoclonal antibody. The excellent selectivity of the polyclonal antibodies was comprehensively examined by determining the cross‐reactivity to common explosives and other nitroaromatics including nitro musk components. The widely held belief that polyclonal antibodies generally display higher cross‐reactivities than monoclonals could be disproved. Copyright © 2012 John Wiley & Sons, Ltd." @default.
• W2099376237 created "2016-06-24" @default.
• W2099376237 creator A5031780710 @default.
• W2099376237 creator A5089404192 @default.
• W2099376237 date "2012-01-30" @default.
• W2099376237 modified "2023-09-27" @default.
• W2099376237 title "Extremely sensitive and selective antibodies against the explosive 2,4,6-trinitrotoluene by rational design of a structurally optimized hapten" @default.
• W2099376237 cites W1519871189 @default.
• W2099376237 cites W1973633277 @default.
• W2099376237 cites W1980072265 @default.
• W2099376237 cites W1986076948 @default.
• W2099376237 cites W1997133987 @default.
• W2099376237 cites W2007963893 @default.
• W2099376237 cites W2009884990 @default.
• W2099376237 cites W2021675084 @default.
• W2099376237 cites W2026587806 @default.
• W2099376237 cites W2037281719 @default.
• W2099376237 cites W2039149399 @default.
• W2099376237 cites W2070816244 @default.
• W2099376237 cites W2072694137 @default.
• W2099376237 cites W2078508078 @default.
• W2099376237 cites W2081099854 @default.
• W2099376237 cites W2094514305 @default.
• W2099376237 cites W2100084730 @default.
• W2099376237 cites W4302416229 @default.
• W2099376237 doi "https://doi.org/10.1002/jmr.2162" @default.
• W2099376237 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22290770" @default.
• W2099376237 hasPublicationYear "2012" @default.
• W2099376237 type Work @default.
• W2099376237 sameAs 2099376237 @default.
• W2099376237 citedByCount "25" @default.
• W2099376237 countsByYear W20993762372012 @default.
• W2099376237 countsByYear W20993762372013 @default.
• W2099376237 countsByYear W20993762372014 @default.
• W2099376237 countsByYear W20993762372015 @default.
• W2099376237 countsByYear W20993762372016 @default.
• W2099376237 countsByYear W20993762372017 @default.
• W2099376237 countsByYear W20993762372018 @default.
• W2099376237 countsByYear W20993762372019 @default.
• W2099376237 countsByYear W20993762372020 @default.
• W2099376237 countsByYear W20993762372021 @default.
• W2099376237 countsByYear W20993762372023 @default.
• W2099376237 crossrefType "journal-article" @default.
• W2099376237 hasAuthorship W2099376237A5031780710 @default.
• W2099376237 hasAuthorship W2099376237A5089404192 @default.
• W2099376237 hasConcept C119128265 @default.
• W2099376237 hasConcept C134306372 @default.
• W2099376237 hasConcept C154238967 @default.
• W2099376237 hasConcept C159654299 @default.
• W2099376237 hasConcept C160756335 @default.
• W2099376237 hasConcept C178790620 @default.
• W2099376237 hasConcept C185592680 @default.
• W2099376237 hasConcept C197336794 @default.
• W2099376237 hasConcept C203014093 @default.
• W2099376237 hasConcept C2780420688 @default.
• W2099376237 hasConcept C2910635913 @default.
• W2099376237 hasConcept C29144244 @default.
• W2099376237 hasConcept C33923547 @default.
• W2099376237 hasConcept C43617362 @default.
• W2099376237 hasConcept C55493867 @default.
• W2099376237 hasConcept C86803240 @default.
• W2099376237 hasConcept C86807702 @default.
• W2099376237 hasConcept C956191 @default.
• W2099376237 hasConceptScore W2099376237C119128265 @default.
• W2099376237 hasConceptScore W2099376237C134306372 @default.
• W2099376237 hasConceptScore W2099376237C154238967 @default.
• W2099376237 hasConceptScore W2099376237C159654299 @default.
• W2099376237 hasConceptScore W2099376237C160756335 @default.
• W2099376237 hasConceptScore W2099376237C178790620 @default.
• W2099376237 hasConceptScore W2099376237C185592680 @default.
• W2099376237 hasConceptScore W2099376237C197336794 @default.
• W2099376237 hasConceptScore W2099376237C203014093 @default.
• W2099376237 hasConceptScore W2099376237C2780420688 @default.
• W2099376237 hasConceptScore W2099376237C2910635913 @default.
• W2099376237 hasConceptScore W2099376237C29144244 @default.
• W2099376237 hasConceptScore W2099376237C33923547 @default.
• W2099376237 hasConceptScore W2099376237C43617362 @default.
• W2099376237 hasConceptScore W2099376237C55493867 @default.
• W2099376237 hasConceptScore W2099376237C86803240 @default.
• W2099376237 hasConceptScore W2099376237C86807702 @default.
• W2099376237 hasConceptScore W2099376237C956191 @default.
• W2099376237 hasIssue "2" @default.
• W2099376237 hasLocation W20993762371 @default.
• W2099376237 hasLocation W20993762372 @default.
• W2099376237 hasOpenAccess W2099376237 @default.
• W2099376237 hasPrimaryLocation W20993762371 @default.
• W2099376237 hasRelatedWork W1972893657 @default.
• W2099376237 hasRelatedWork W1986320371 @default.
• W2099376237 hasRelatedWork W2009008500 @default.
• W2099376237 hasRelatedWork W2011949324 @default.
• W2099376237 hasRelatedWork W2030657907 @default.
• W2099376237 hasRelatedWork W2044573037 @default.
• W2099376237 hasRelatedWork W2090741034 @default.
• W2099376237 hasRelatedWork W2408090127 @default.
• W2099376237 hasRelatedWork W892782301 @default.
• W2099376237 hasRelatedWork W1985098886 @default.
• W2099376237 hasVolume "25" @default.
• W2099376237 isParatext "false" @default.

• next