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• W2099396543 abstract "We identified a near-full-length cDNA clone encoding ornithine decarboxylase (ODC) from tomato (Lycopersicon esculentum Mill). It contained a small upstream open reading frame (uORF) within its 5' untranslated region. An in vitro translation assay demonstrated that the uORF repressed expression of downstream ORF. Neither nucleotide nor predicted peptide sequence of the uORF was responsible for the repression. The presence of upstream AUG codon was shown to be responsible. ODC expression appeared to be organ specific. The ODC gene was expressed in roots, hypocotyls and sink leaves but not in source leaves. ODC transcripts were observed in apical meristem of primary roots, and were distributed in cells of cortex layer preferentially. ODC expression responded immediately to sucrose availability via the sucrose-specific pathway independent of hexokinase. Sucrose induction of ODC gene was seen in roots, hypocotyls and flowers but not in mature leaves. Moreover, only the root apical meristem responded to sucrose availability. These observations indicate that the spatial pattern of ODC expression is closely associated with cell proliferation and that sucrose sensing plays a major role in the spatial pattern of ODC expression. Also, the differential regulation of ODC and arginine decarboxylase gene expression by factors modulating plant growth suggests that they would have different physiological roles in plant development." @default.
• W2099396543 created "2016-06-24" @default.
• W2099396543 creator A5032613159 @default.
• W2099396543 creator A5047026843 @default.
• W2099396543 date "2001-03-15" @default.
• W2099396543 modified "2023-10-15" @default.
• W2099396543 title "The Regulation of Ornithine Decarboxylase Gene Expression by Sucrose and Small Upstream Open Reading Frame in Tomato (Lycopersicon esculentum Mill)" @default.
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• W2099396543 doi "https://doi.org/10.1093/pcp/pce040" @default.
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