FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2099450421> ?p ?o ?g. }

• W2099450421 endingPage "690" @default.
• W2099450421 startingPage "677" @default.
• W2099450421 abstract "Abstract A peptide corresponding to residues 101–141 of the human nucleotide excision repair protein XPA was synthesized with an isoleucine substituted for L138 and its solution structure studied by circular dichroism and homonuclear 1H NMR spectroscopy. The peptide, (XPA-41), contains a C4−type zinc-binding motif, C105-(X)2C108-(X)l7−C126-(X)2 C129, which XPA requires for damaged-DNA binding activity. The proton resonances of XPA-41without zinc (apoXPA-41) were assigned using homonuclear TOCSY, NOESY and DQF-COSY data and show the apo-zinc peptide is a random coil. The peptide was folded with the addition of 1.2 equivalents of ZnCl2 in dilute solution at pH 4.0. Electrospray ionization mass spectroscopy illustrated an increase in the molecular weight of XPA-41 by 65 amu. Circular dichroism spectra of the zinc-folded peptide (zXPA-41) showed the acquisition of elements of secondary structure. Such a conclusion was confirmed with'H NMR data collected at 25°C, pH 6.3. Hα-secondary shifts and NOE patterns indicate that regions V102-C105 and G109-F112 form an anti-parallel β-sheet and residues N128-K137 form a nascent α-helix. Rapid exchange of most amide resonances between S115-C126 prohibited unambiguous assignment of all the proton resonances in this region. However, a 1.19 ppm downfield shift of the Hα resonance of T125 relative to the apo-zinc peptide, together with downfield shifted Hα resonances for the adjacent residues (P124 and L123), suggest a second β-sheet is present in the S115-C126 region. On the basis of structural similarities to GATA-1 (Science 267:438–446), a homology generated structure for zXPA-41 was made, using GATA-1 as the template, which satisfied all the observed NOEs. Using the hybrid homology-NMR based zXPA-41 structure and analogy to GATA-1, models for the role played by the zinc-binding core (101–141) of XPA in DNA damage recognition are proposed." @default.
• W2099450421 created "2016-06-24" @default.
• W2099450421 creator A5033465444 @default.
• W2099450421 creator A5069241881 @default.
• W2099450421 date "1997-06-01" @default.
• W2099450421 modified "2023-09-23" @default.
• W2099450421 title "Human Nucleotide Excision Repair Protein XPA:1H NMR and CD Solution Studies of a Synthetic Peptide Fragment Corresponding to the Zinc-Binding Domain (101–141)" @default.
• W2099450421 cites W12794080 @default.
• W2099450421 cites W1499802317 @default.
• W2099450421 cites W1539339671 @default.
• W2099450421 cites W1956254450 @default.
• W2099450421 cites W1969760979 @default.
• W2099450421 cites W1969769040 @default.
• W2099450421 cites W1970417043 @default.
• W2099450421 cites W1971966571 @default.
• W2099450421 cites W1972459558 @default.
• W2099450421 cites W1981147325 @default.
• W2099450421 cites W1982034145 @default.
• W2099450421 cites W1989317177 @default.
• W2099450421 cites W1992661805 @default.
• W2099450421 cites W1993844842 @default.
• W2099450421 cites W1995021789 @default.
• W2099450421 cites W1997354900 @default.
• W2099450421 cites W1999104040 @default.
• W2099450421 cites W2000856801 @default.
• W2099450421 cites W2000936373 @default.
• W2099450421 cites W2010035436 @default.
• W2099450421 cites W2017639307 @default.
• W2099450421 cites W2020651808 @default.
• W2099450421 cites W2020745281 @default.
• W2099450421 cites W2024275778 @default.
• W2099450421 cites W2030901966 @default.
• W2099450421 cites W2034711659 @default.
• W2099450421 cites W2035288562 @default.
• W2099450421 cites W2037692676 @default.
• W2099450421 cites W2045950270 @default.
• W2099450421 cites W2053539431 @default.
• W2099450421 cites W2058425350 @default.
• W2099450421 cites W2067127132 @default.
• W2099450421 cites W2067470404 @default.
• W2099450421 cites W2068685168 @default.
• W2099450421 cites W2074770586 @default.
• W2099450421 cites W2075954862 @default.
• W2099450421 cites W2078882775 @default.
• W2099450421 cites W2081049803 @default.
• W2099450421 cites W2085213735 @default.
• W2099450421 cites W2085242657 @default.
• W2099450421 cites W2091566576 @default.
• W2099450421 cites W2133335149 @default.
• W2099450421 cites W2134925852 @default.
• W2099450421 cites W2142355539 @default.
• W2099450421 cites W2172093339 @default.
• W2099450421 cites W2178040892 @default.
• W2099450421 cites W2334261900 @default.
• W2099450421 cites W2949250147 @default.
• W2099450421 cites W35241519 @default.
• W2099450421 cites W4205408780 @default.
• W2099450421 cites W4251299197 @default.
• W2099450421 doi "https://doi.org/10.1080/07391102.1997.10508171" @default.
• W2099450421 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9195337" @default.
• W2099450421 hasPublicationYear "1997" @default.
• W2099450421 type Work @default.
• W2099450421 sameAs 2099450421 @default.
• W2099450421 citedByCount "5" @default.
• W2099450421 crossrefType "journal-article" @default.
• W2099450421 hasAuthorship W2099450421A5033465444 @default.
• W2099450421 hasAuthorship W2099450421A5069241881 @default.
• W2099450421 hasConcept C133571119 @default.
• W2099450421 hasConcept C178790620 @default.
• W2099450421 hasConcept C185592680 @default.
• W2099450421 hasConcept C2779281246 @default.
• W2099450421 hasConcept C32909587 @default.
• W2099450421 hasConcept C34349673 @default.
• W2099450421 hasConcept C40447573 @default.
• W2099450421 hasConcept C535196362 @default.
• W2099450421 hasConcept C55493867 @default.
• W2099450421 hasConcept C62614982 @default.
• W2099450421 hasConcept C66974803 @default.
• W2099450421 hasConcept C71240020 @default.
• W2099450421 hasConcept C8010536 @default.
• W2099450421 hasConceptScore W2099450421C133571119 @default.
• W2099450421 hasConceptScore W2099450421C178790620 @default.
• W2099450421 hasConceptScore W2099450421C185592680 @default.
• W2099450421 hasConceptScore W2099450421C2779281246 @default.
• W2099450421 hasConceptScore W2099450421C32909587 @default.
• W2099450421 hasConceptScore W2099450421C34349673 @default.
• W2099450421 hasConceptScore W2099450421C40447573 @default.
• W2099450421 hasConceptScore W2099450421C535196362 @default.
• W2099450421 hasConceptScore W2099450421C55493867 @default.
• W2099450421 hasConceptScore W2099450421C62614982 @default.
• W2099450421 hasConceptScore W2099450421C66974803 @default.
• W2099450421 hasConceptScore W2099450421C71240020 @default.
• W2099450421 hasConceptScore W2099450421C8010536 @default.
• W2099450421 hasIssue "6" @default.
• W2099450421 hasLocation W20994504211 @default.
• W2099450421 hasLocation W20994504212 @default.
• W2099450421 hasOpenAccess W2099450421 @default.
• W2099450421 hasPrimaryLocation W20994504211 @default.

• next