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- W2099502610 abstract "A Tn7 donor plasmid, pTn7SX, was constructed for use with the model gram-positive bacterium Bacillus subtilis. This new mini-Tn7, mTn7SX, contains a spectinomycin resistance cassette and an outward-facing, xylose-inducible promoter, thereby allowing for the regulated expression of genes downstream of the transposon. We demonstrate that mTn7SX inserts are obtained at a high frequency and occur randomly throughout the B. subtilis genome. The utility of this system was demonstrated by the selection of mutants with increased resistance to the antibiotic fosfomycin or duramycin." @default.
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- W2099502610 date "2008-06-01" @default.
- W2099502610 modified "2023-09-30" @default.
- W2099502610 title "In Vitro Mutagenesis of Bacillus subtilis by Using a Modified Tn 7 Transposon with an Outward-Facing Inducible Promoter" @default.
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- W2099502610 doi "https://doi.org/10.1128/aem.00476-08" @default.
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