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- W2099508777 abstract "A marine Yarrowia lipolytica yeast, named Bohaisea-9145, was found to secrete a large amount of lipase into the medium. A gene coding for lipase was cloned from the genome of this strain and expressed successfully in Escherichia coli BL21 (DE3). A maximum activity of 17.6 U/mg was obtained from cellular extract of E. coli harboring the lipase gene. The recombinant lipase exhibits one band with a molecular mass of about 44 kDa on SDS-PAGE. The optimal temperature and pH of the purified lipase were 35°C and 8.5, respectively. The K m and V max values of the lipase for p-nitrophenyl laurate were 0.582 μM and 0.124 mmol min−1 mg−1 under 35°C, respectively. Additionally, the purified lipase showed a high activity and stability over a wide range of temperatures, especially in the low and moderate temperatures, suggesting its potential for industrial applications." @default.
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- W2099508777 date "2011-11-10" @default.
- W2099508777 modified "2023-10-16" @default.
- W2099508777 title "Cloning, characterization and expression of a novel lipase gene from marine psychrotrophic Yarrowia lipolytica" @default.
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- W2099508777 doi "https://doi.org/10.1007/s13213-011-0348-9" @default.
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