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• W2099576192 endingPage "2531" @default.
• W2099576192 startingPage "2521" @default.
• W2099576192 abstract "The creation of a vascularized bed makes the survival of seeded cells on 3-dimensional scaffolds much more likely. However, relying purely on random capillary ingrowth into the porous scaffolds from the host may compromise vascularization of a scaffold. One solution is to transplant cells capable of differentiating into new blood vessels into the scaffolds to accelerate the creation of a vascularized scaffold. Because endothelial cells are the key cells involved in blood vessel formation, the present study was designed to investigate the development of a biomaterial surface that supports endothelial cell attachment and proliferation. The subsequent effects of the material surface modifications on the differentiation and proliferation of human bone marrow-derived fibroblasts (HBMFs) when grown in co-culture with a human bone marrow endothelial cell line (HBMEC-60) were studied. Endothelialization studies showed that the gelatin-coated and hydroxyapatite-coated substrates were superior for HBMEC-60 attachment and proliferation to hydrolyzed-only or untreated polycaprolactone substrates. Co-culture studies showed that the presence of the HBMEC-60 specifically enhanced HBMF cell proliferation and differentiation and that this effect was not observed with co-culture with skin fibroblasts. It is concluded that the co-culture of endothelial cells with HBMFs could be a promising culture system for bone tissue- engineering applications." @default.
• W2099576192 created "2016-06-24" @default.
• W2099576192 creator A5003215109 @default.
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• W2099576192 date "2006-09-01" @default.
• W2099576192 modified "2023-10-16" @default.
• W2099576192 title "Co-culture of Bone Marrow Fibroblasts and Endothelial Cells on Modified Polycaprolactone Substrates for Enhanced Potentials in Bone Tissue Engineering" @default.
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• W2099576192 doi "https://doi.org/10.1089/ten.2006.12.2521" @default.
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