FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2099661054> ?p ?o ?g. }

• W2099661054 abstract "Microscopy and antigen detecting rapid diagnostic tests are the diagnostic tests of choice in management of clinical malaria. However, due to their limitations, the need to utilize more sensitive methods such as real-time PCR (qPCR) is evident as more studies are now utilizing molecular methods in detection of malaria. Some of the challenges that continue to limit the widespread utilization of qPCR include lack of assay standardization, assay variability, risk of contamination, and the need for cold-chain. Lyophilization of molecular assays can overcome some of these limitations and potentially enable widespread qPCR utilization.A recently published multiplex malaria qPCR assay was lyophilized by freezing drying into Sample-Ready™ format (MMSR). MMSR assay contained all the required reagents for qPCR including primers and probes, requiring only the addition of water and sample to perform qPCR. The performance of the MMSR assay was compared to the non-freeze dried, wet assay. Stability studies were done by maintaining the MMSR assays at four different ambient temperatures of 4°C, room temperature (RT), 37°C and 42°C over a period of 42 days, tested at seven-day intervals. Plasmodium falciparum and Plasmodium vivax DNAs were used for analysis of the MMSR assay either as single or mixed parasites, at two different concentrations. The CT values and the standard deviations (SD) were used in the analysis of the assay performance.The limit of detection for the MMSR assay was 0.244 parasites/μL for Plasmodium spp. (PLU) and P. falciparum (FAL) assay targets compared to wet assay which was 0.39 and 3.13 parasites/μL for PLU and FAL assay targets, respectively. The MMSR assay performed with high efficiencies similar to those of the wet assay and was stable at 37°C for 42 days, with estimated shelf-life of 5 months. When used to analyse field clinical samples, MMSR assay performed with 100% sensitivity and specificity compared to the wet assay.The MMSR assay has the same robust performance characteristics as the wet assay and is highly stable. Availability of MMSR assay allows flexibility and provides an option in choosing assay for malaria diagnostics depending on the application, needs and budget." @default.
• W2099661054 created "2016-06-24" @default.
• W2099661054 creator A5019928797 @default.
• W2099661054 creator A5045725145 @default.
• W2099661054 creator A5059493447 @default.
• W2099661054 creator A5059907819 @default.
• W2099661054 creator A5063733503 @default.
• W2099661054 creator A5069843611 @default.
• W2099661054 creator A5074945505 @default.
• W2099661054 creator A5077124817 @default.
• W2099661054 creator A5080307493 @default.
• W2099661054 date "2014-04-25" @default.
• W2099661054 modified "2023-10-16" @default.
• W2099661054 title "Sample-ready multiplex qPCR assay for detection of malaria" @default.
• W2099661054 cites W1856096155 @default.
• W2099661054 cites W1990827269 @default.
• W2099661054 cites W1995572691 @default.
• W2099661054 cites W2004995441 @default.
• W2099661054 cites W2010146342 @default.
• W2099661054 cites W2042573261 @default.
• W2099661054 cites W2053248925 @default.
• W2099661054 cites W2069995906 @default.
• W2099661054 cites W2071195067 @default.
• W2099661054 cites W2088824647 @default.
• W2099661054 cites W2096357035 @default.
• W2099661054 cites W2107255366 @default.
• W2099661054 cites W2108065348 @default.
• W2099661054 cites W2131478872 @default.
• W2099661054 cites W2147509508 @default.
• W2099661054 cites W2164752189 @default.
• W2099661054 cites W2165822060 @default.
• W2099661054 cites W2168420558 @default.
• W2099661054 cites W2169506054 @default.
• W2099661054 cites W4239427216 @default.
• W2099661054 doi "https://doi.org/10.1186/1475-2875-13-158" @default.
• W2099661054 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4026594" @default.
• W2099661054 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24767409" @default.
• W2099661054 hasPublicationYear "2014" @default.
• W2099661054 type Work @default.
• W2099661054 sameAs 2099661054 @default.
• W2099661054 citedByCount "25" @default.
• W2099661054 countsByYear W20996610542015 @default.
• W2099661054 countsByYear W20996610542016 @default.
• W2099661054 countsByYear W20996610542017 @default.
• W2099661054 countsByYear W20996610542018 @default.
• W2099661054 countsByYear W20996610542019 @default.
• W2099661054 countsByYear W20996610542020 @default.
• W2099661054 countsByYear W20996610542021 @default.
• W2099661054 countsByYear W20996610542022 @default.
• W2099661054 countsByYear W20996610542023 @default.
• W2099661054 crossrefType "journal-article" @default.
• W2099661054 hasAuthorship W2099661054A5019928797 @default.
• W2099661054 hasAuthorship W2099661054A5045725145 @default.
• W2099661054 hasAuthorship W2099661054A5059493447 @default.
• W2099661054 hasAuthorship W2099661054A5059907819 @default.
• W2099661054 hasAuthorship W2099661054A5063733503 @default.
• W2099661054 hasAuthorship W2099661054A5069843611 @default.
• W2099661054 hasAuthorship W2099661054A5074945505 @default.
• W2099661054 hasAuthorship W2099661054A5077124817 @default.
• W2099661054 hasAuthorship W2099661054A5080307493 @default.
• W2099661054 hasBestOaLocation W20996610541 @default.
• W2099661054 hasConcept C119128265 @default.
• W2099661054 hasConcept C153911025 @default.
• W2099661054 hasConcept C159047783 @default.
• W2099661054 hasConcept C185592680 @default.
• W2099661054 hasConcept C190612196 @default.
• W2099661054 hasConcept C203014093 @default.
• W2099661054 hasConcept C2778048844 @default.
• W2099661054 hasConcept C2778371730 @default.
• W2099661054 hasConcept C2779997623 @default.
• W2099661054 hasConcept C2781188995 @default.
• W2099661054 hasConcept C43617362 @default.
• W2099661054 hasConcept C60644358 @default.
• W2099661054 hasConcept C86803240 @default.
• W2099661054 hasConcept C90856448 @default.
• W2099661054 hasConceptScore W2099661054C119128265 @default.
• W2099661054 hasConceptScore W2099661054C153911025 @default.
• W2099661054 hasConceptScore W2099661054C159047783 @default.
• W2099661054 hasConceptScore W2099661054C185592680 @default.
• W2099661054 hasConceptScore W2099661054C190612196 @default.
• W2099661054 hasConceptScore W2099661054C203014093 @default.
• W2099661054 hasConceptScore W2099661054C2778048844 @default.
• W2099661054 hasConceptScore W2099661054C2778371730 @default.
• W2099661054 hasConceptScore W2099661054C2779997623 @default.
• W2099661054 hasConceptScore W2099661054C2781188995 @default.
• W2099661054 hasConceptScore W2099661054C43617362 @default.
• W2099661054 hasConceptScore W2099661054C60644358 @default.
• W2099661054 hasConceptScore W2099661054C86803240 @default.
• W2099661054 hasConceptScore W2099661054C90856448 @default.
• W2099661054 hasIssue "1" @default.
• W2099661054 hasLocation W20996610541 @default.
• W2099661054 hasLocation W20996610542 @default.
• W2099661054 hasLocation W20996610543 @default.
• W2099661054 hasLocation W20996610544 @default.
• W2099661054 hasOpenAccess W2099661054 @default.
• W2099661054 hasPrimaryLocation W20996610541 @default.
• W2099661054 hasRelatedWork W2100262490 @default.
• W2099661054 hasRelatedWork W2126740217 @default.
• W2099661054 hasRelatedWork W2145726869 @default.
• W2099661054 hasRelatedWork W2147272002 @default.

• next