Matches in SemOpenAlex for { <https://semopenalex.org/work/W2099681791> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2099681791 endingPage "263" @default.
- W2099681791 startingPage "259" @default.
- W2099681791 abstract "It is difficult to purify DNA from mature tree leaves at the end of the growing season, because of their thick cell wall, and high content in polysaccharides, phenolic compounds and endonucleases. A simple, fast and efficient method for DNA purification from 100 mg fresh weight leaf samples is described here. It has been developed for extracting DNA from mature leaves of Quercus, Fraxinus Prunus and Acer. The protocol is a modified CTAB (hexadecyltrimethylammonium bromide) method including a combina- tion of β-mercaptoethanol, polyvinylpyrrolidone, sodium dodecyl sulfate and lithium chloride including short centrifugation runs. It is very efficient yielding up to 950 μg DNA/g of fresh weight, even when very mature leaves are processed. The extracted DNA was used as template to characterise oaks by microsatellite analysis. Its efficiency has been compared to four commercially available kits and two other published. CTAB protocols. The protocol is also inexpensive compared to commercial kits. (© Inra/Elsevier, Paris.)" @default.
- W2099681791 created "2016-06-24" @default.
- W2099681791 creator A5013154137 @default.
- W2099681791 creator A5063155180 @default.
- W2099681791 date "1999-01-01" @default.
- W2099681791 modified "2023-10-12" @default.
- W2099681791 title "An efficient micro-method of DNA isolation from mature leaves of four hardwood tree species Acer, Fraxinus, Prunus and Quercus" @default.
- W2099681791 cites W2026913927 @default.
- W2099681791 doi "https://doi.org/10.1051/forest:19990308" @default.
- W2099681791 hasPublicationYear "1999" @default.
- W2099681791 type Work @default.
- W2099681791 sameAs 2099681791 @default.
- W2099681791 citedByCount "156" @default.
- W2099681791 countsByYear W20996817912012 @default.
- W2099681791 countsByYear W20996817912013 @default.
- W2099681791 countsByYear W20996817912014 @default.
- W2099681791 countsByYear W20996817912015 @default.
- W2099681791 countsByYear W20996817912016 @default.
- W2099681791 countsByYear W20996817912017 @default.
- W2099681791 countsByYear W20996817912018 @default.
- W2099681791 countsByYear W20996817912019 @default.
- W2099681791 countsByYear W20996817912020 @default.
- W2099681791 countsByYear W20996817912021 @default.
- W2099681791 countsByYear W20996817912022 @default.
- W2099681791 countsByYear W20996817912023 @default.
- W2099681791 crossrefType "journal-article" @default.
- W2099681791 hasAuthorship W2099681791A5013154137 @default.
- W2099681791 hasAuthorship W2099681791A5063155180 @default.
- W2099681791 hasBestOaLocation W20996817911 @default.
- W2099681791 hasConcept C144027150 @default.
- W2099681791 hasConcept C2775941552 @default.
- W2099681791 hasConcept C2778934191 @default.
- W2099681791 hasConcept C2779692143 @default.
- W2099681791 hasConcept C2780674770 @default.
- W2099681791 hasConcept C59822182 @default.
- W2099681791 hasConcept C86803240 @default.
- W2099681791 hasConcept C89423630 @default.
- W2099681791 hasConceptScore W2099681791C144027150 @default.
- W2099681791 hasConceptScore W2099681791C2775941552 @default.
- W2099681791 hasConceptScore W2099681791C2778934191 @default.
- W2099681791 hasConceptScore W2099681791C2779692143 @default.
- W2099681791 hasConceptScore W2099681791C2780674770 @default.
- W2099681791 hasConceptScore W2099681791C59822182 @default.
- W2099681791 hasConceptScore W2099681791C86803240 @default.
- W2099681791 hasConceptScore W2099681791C89423630 @default.
- W2099681791 hasIssue "3" @default.
- W2099681791 hasLocation W20996817911 @default.
- W2099681791 hasLocation W20996817912 @default.
- W2099681791 hasLocation W20996817913 @default.
- W2099681791 hasLocation W20996817914 @default.
- W2099681791 hasOpenAccess W2099681791 @default.
- W2099681791 hasPrimaryLocation W20996817911 @default.
- W2099681791 hasRelatedWork W166776715 @default.
- W2099681791 hasRelatedWork W2034420173 @default.
- W2099681791 hasRelatedWork W2094330411 @default.
- W2099681791 hasRelatedWork W2283358449 @default.
- W2099681791 hasRelatedWork W2372738923 @default.
- W2099681791 hasRelatedWork W322437757 @default.
- W2099681791 hasRelatedWork W4233255859 @default.
- W2099681791 hasRelatedWork W4233296972 @default.
- W2099681791 hasRelatedWork W4244477645 @default.
- W2099681791 hasRelatedWork W4310650695 @default.
- W2099681791 hasVolume "56" @default.
- W2099681791 isParatext "false" @default.
- W2099681791 isRetracted "false" @default.
- W2099681791 magId "2099681791" @default.
- W2099681791 workType "article" @default.