FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2099685008> ?p ?o ?g. }

• W2099685008 endingPage "15240" @default.
• W2099685008 startingPage "15235" @default.
• W2099685008 abstract "The RtcB protein has recently been identified as a 3′-phosphate RNA ligase that directly joins an RNA strand ending with a 2′,3′-cyclic phosphate to the 5′-hydroxyl group of another RNA strand in a GTP/Mn 2+ -dependent reaction. Here, we report two crystal structures of Pyrococcus horikoshii RNA-splicing ligase RtcB in complex with Mn 2+ alone (RtcB/ Mn 2+ ) and together with a covalently bound GMP (RtcB-GMP/Mn 2+ ). The RtcB/ Mn 2+ structure (at 1.6 Å resolution) shows two Mn 2+ ions at the active site, and an array of sulfate ions nearby that indicate the binding sites of the RNA phosphate backbone. The structure of the RtcB-GMP/Mn 2+ complex (at 2.3 Å resolution) reveals the detailed geometry of guanylylation of histidine 404. The critical roles of the key residues involved in the binding of the two Mn 2+ ions, the four sulfates, and GMP are validated in extensive mutagenesis and biochemical experiments, which also provide a thorough characterization for the three steps of the RtcB ligation pathway: ( i ) guanylylation of the enzyme, ( ii ) guanylyl-transfer to the RNA substrate, and ( iii ) overall ligation. These results demonstrate that the enzyme’s substrate-induced GTP binding site and the putative reactive RNA ends are in the vicinity of the binuclear Mn 2+ active center, which provides detailed insight into how the enzyme-bound GMP is tansferred to the 3′-phosphate of the RNA substrate for activation and subsequent nucleophilic attack by the 5′-hydroxyl of the second RNA substrate, resulting in the ligated product and release of GMP." @default.
• W2099685008 created "2016-06-24" @default.
• W2099685008 creator A5006042602 @default.
• W2099685008 creator A5010384396 @default.
• W2099685008 creator A5022233637 @default.
• W2099685008 creator A5033720199 @default.
• W2099685008 creator A5038581059 @default.
• W2099685008 creator A5048145842 @default.
• W2099685008 creator A5060425260 @default.
• W2099685008 creator A5063450287 @default.
• W2099685008 creator A5066627537 @default.
• W2099685008 creator A5084926998 @default.
• W2099685008 date "2012-09-04" @default.
• W2099685008 modified "2023-10-01" @default.
• W2099685008 title "Structural and mechanistic insights into guanylylation of RNA-splicing ligase RtcB joining RNA between 3′-terminal phosphate and 5′-OH" @default.
• W2099685008 cites W172203261 @default.
• W2099685008 cites W1780093098 @default.
• W2099685008 cites W1967653717 @default.
• W2099685008 cites W1969467746 @default.
• W2099685008 cites W1970997159 @default.
• W2099685008 cites W1972915201 @default.
• W2099685008 cites W1977824655 @default.
• W2099685008 cites W1978547782 @default.
• W2099685008 cites W1979657493 @default.
• W2099685008 cites W1986327332 @default.
• W2099685008 cites W1990502537 @default.
• W2099685008 cites W1993813359 @default.
• W2099685008 cites W1996223053 @default.
• W2099685008 cites W1996810566 @default.
• W2099685008 cites W2005463994 @default.
• W2099685008 cites W2012304127 @default.
• W2099685008 cites W2020859487 @default.
• W2099685008 cites W2033387234 @default.
• W2099685008 cites W2035875913 @default.
• W2099685008 cites W2040584193 @default.
• W2099685008 cites W2040993603 @default.
• W2099685008 cites W2041421251 @default.
• W2099685008 cites W2065232441 @default.
• W2099685008 cites W2065942265 @default.
• W2099685008 cites W2076156469 @default.
• W2099685008 cites W2076317271 @default.
• W2099685008 cites W2081407431 @default.
• W2099685008 cites W2081451822 @default.
• W2099685008 cites W2106246537 @default.
• W2099685008 cites W2123261396 @default.
• W2099685008 cites W2136356801 @default.
• W2099685008 cites W2162586031 @default.
• W2099685008 doi "https://doi.org/10.1073/pnas.1213795109" @default.
• W2099685008 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3458315" @default.
• W2099685008 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22949672" @default.
• W2099685008 hasPublicationYear "2012" @default.
• W2099685008 type Work @default.
• W2099685008 sameAs 2099685008 @default.
• W2099685008 citedByCount "47" @default.
• W2099685008 countsByYear W20996850082013 @default.
• W2099685008 countsByYear W20996850082014 @default.
• W2099685008 countsByYear W20996850082015 @default.
• W2099685008 countsByYear W20996850082016 @default.
• W2099685008 countsByYear W20996850082017 @default.
• W2099685008 countsByYear W20996850082018 @default.
• W2099685008 countsByYear W20996850082019 @default.
• W2099685008 countsByYear W20996850082020 @default.
• W2099685008 countsByYear W20996850082021 @default.
• W2099685008 countsByYear W20996850082022 @default.
• W2099685008 countsByYear W20996850082023 @default.
• W2099685008 crossrefType "journal-article" @default.
• W2099685008 hasAuthorship W2099685008A5006042602 @default.
• W2099685008 hasAuthorship W2099685008A5010384396 @default.
• W2099685008 hasAuthorship W2099685008A5022233637 @default.
• W2099685008 hasAuthorship W2099685008A5033720199 @default.
• W2099685008 hasAuthorship W2099685008A5038581059 @default.
• W2099685008 hasAuthorship W2099685008A5048145842 @default.
• W2099685008 hasAuthorship W2099685008A5060425260 @default.
• W2099685008 hasAuthorship W2099685008A5063450287 @default.
• W2099685008 hasAuthorship W2099685008A5066627537 @default.
• W2099685008 hasAuthorship W2099685008A5084926998 @default.
• W2099685008 hasBestOaLocation W20996850081 @default.
• W2099685008 hasConcept C104317684 @default.
• W2099685008 hasConcept C1342335 @default.
• W2099685008 hasConcept C175114707 @default.
• W2099685008 hasConcept C181199279 @default.
• W2099685008 hasConcept C185592680 @default.
• W2099685008 hasConcept C2778332380 @default.
• W2099685008 hasConcept C2909068217 @default.
• W2099685008 hasConcept C41183919 @default.
• W2099685008 hasConcept C552990157 @default.
• W2099685008 hasConcept C55493867 @default.
• W2099685008 hasConcept C67705224 @default.
• W2099685008 hasConcept C71240020 @default.
• W2099685008 hasConcept C76346623 @default.
• W2099685008 hasConceptScore W2099685008C104317684 @default.
• W2099685008 hasConceptScore W2099685008C1342335 @default.
• W2099685008 hasConceptScore W2099685008C175114707 @default.
• W2099685008 hasConceptScore W2099685008C181199279 @default.
• W2099685008 hasConceptScore W2099685008C185592680 @default.
• W2099685008 hasConceptScore W2099685008C2778332380 @default.
• W2099685008 hasConceptScore W2099685008C2909068217 @default.
• W2099685008 hasConceptScore W2099685008C41183919 @default.

• next