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- W2099686913 abstract "st1:*{behavior:url(#ieooui) } /* Style Definitions */ table.MsoNormalTable{mso-style-name:Table Normal;mso-tstyle-rowband-size:0;mso-tstyle-colband-size:0;mso-style-noshow:yes;mso-style-parent:;mso-padding-alt:0cm 5.4pt 0cm 5.4pt;mso-para-margin:0cm;mso-para-margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:10.0pt;font-family:Times New Roman;mso-ansi-language:#0400;mso-fareast-language:#0400;mso-bidi-language:#0400;} A matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer has been constructed at the Department of Physics, University of Colombo, Sri Lanka. In this mass spectrometer, a solid sample which contains analyte molecules embedded in a suitable matrix compound in excess is exposed to a UV pulsed nitrogen laser source (λ = 337 nm). The desorbed secondary ions from the sample are mass analyzed using the time-of-flight technique. The first MALDI-TOF mass spectra of two peptides namely [tyr 8 ]-bradykinin (1076.2 u) and porcine N-acetyl renin substrate tetradecapeptide (1801.1 u) with the matrix 2,5 di-hydro benzoic acid (154.1 u) have been obtained. Each peptide produced a MH + peak corresponding to its intact molecular ion with m/q values of 1089 ± 6 and 1794 ± 8 respectively. doi:10.4038/sljp.v1i0.168 Sri Lankan Journal of Physics, Vol.1,(2000) 33-42" @default.
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- W2099686913 date "2000-12-17" @default.
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- W2099686913 title "Construction of a matrix assisted laser desorption/ionization time-of-flight mass spectrometer" @default.
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