FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2099687964> ?p ?o ?g. }

• W2099687964 endingPage "1647" @default.
• W2099687964 startingPage "1638" @default.
• W2099687964 abstract "We describe for the first time the creation of a library of 140 synthetic promoters specifically designed to regulate the expression of recombinant genes in CHO cells. Initially, 10 common viral promoter sequences known to be active in CHO cells were analyzed using bioinformatic sequence analysis programs to determine the identity and relative abundance of transcription factor regulatory elements (TFREs; or transcription factor binding sites) they contained. Based on this, 28 synthetic reporters were constructed that each harbored seven repeats of a discrete TFRE sequence upstream of a minimal CMV core promoter element and secreted alkaline phosphatase (SEAP) reporter gene. After evaluation of the relative activity of TFREs by transient expression in CHO-S cells, we constructed a first generation library of 96 synthetic promoters derived from random ligation of six active TFREs inserted into the same reporter construct backbone. Comparison of the sequence and relative activity of first generation promoters revealed that individual TFRE blocks were either relatively abundant in active promoters (NFκB, E-box), equally distributed across promoters of varying activity (C/EBPα, GC-box) or relatively abundant in low activity promoters (E4F1, CRE). These data were utilized to create a second generation of 44 synthetic promoters based on random ligation of a fixed ratio of 4 TFREs (NFκB 5: E-box 3: C/EBPα 1: GC-box 1). Comparison of the sequence and relative activity of second generation promoters revealed that the most active promoters contained relatively high numbers of both NFκB and E-box TFREs in approximately equal proportion, with a correspondingly low number of GC-box and C/EBPα blocks. The most active second generation promoters achieved approximately twice the activity of a control construct harboring the human cytomegalovirus (CMV) promoter. Lastly, we evaluated the function of a subset of synthetic promoters exhibiting a broad range of activity in different CHO cell host cell lines (CHO-S, CHO-K1, and CHO-DG44) and across extended fed-batch transient expression in CHO-S cells. In general, the different synthetic promoters both maintained their relative activity and the most active promoters consistently and significantly exceeded the activity of the CMV control promoter. For advanced cell engineering strategies our synthetic promoter libraries offer precise control of recombinant transcriptional activity in CHO cells spanning over two orders of magnitude. Biotechnol. Bioeng. 2014;111: 1638–1647. © 2014 Wiley Periodicals, Inc." @default.
• W2099687964 created "2016-06-24" @default.
• W2099687964 creator A5022494700 @default.
• W2099687964 creator A5045290531 @default.
• W2099687964 creator A5051523162 @default.
• W2099687964 creator A5086656543 @default.
• W2099687964 date "2014-05-01" @default.
• W2099687964 modified "2023-10-06" @default.
• W2099687964 title "Synthetic promoters for CHO cell engineering" @default.
• W2099687964 cites W1688835773 @default.
• W2099687964 cites W1932107019 @default.
• W2099687964 cites W1966222319 @default.
• W2099687964 cites W1972382682 @default.
• W2099687964 cites W1975420528 @default.
• W2099687964 cites W1976697406 @default.
• W2099687964 cites W1979371976 @default.
• W2099687964 cites W1980645953 @default.
• W2099687964 cites W1988327779 @default.
• W2099687964 cites W1988370350 @default.
• W2099687964 cites W1993617984 @default.
• W2099687964 cites W1997465134 @default.
• W2099687964 cites W2002091538 @default.
• W2099687964 cites W2019508778 @default.
• W2099687964 cites W2021927081 @default.
• W2099687964 cites W2023666553 @default.
• W2099687964 cites W2028382049 @default.
• W2099687964 cites W2032901225 @default.
• W2099687964 cites W2036228067 @default.
• W2099687964 cites W2038455449 @default.
• W2099687964 cites W2040587998 @default.
• W2099687964 cites W2047381200 @default.
• W2099687964 cites W2048941695 @default.
• W2099687964 cites W2054035212 @default.
• W2099687964 cites W2065045640 @default.
• W2099687964 cites W2068522955 @default.
• W2099687964 cites W2079401546 @default.
• W2099687964 cites W2084549565 @default.
• W2099687964 cites W2090641782 @default.
• W2099687964 cites W2093343938 @default.
• W2099687964 cites W2125882787 @default.
• W2099687964 cites W2127173397 @default.
• W2099687964 cites W2128571608 @default.
• W2099687964 cites W2129656350 @default.
• W2099687964 cites W2140373206 @default.
• W2099687964 cites W2161577298 @default.
• W2099687964 cites W2165268542 @default.
• W2099687964 cites W2171567367 @default.
• W2099687964 doi "https://doi.org/10.1002/bit.25227" @default.
• W2099687964 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24615264" @default.
• W2099687964 hasPublicationYear "2014" @default.
• W2099687964 type Work @default.
• W2099687964 sameAs 2099687964 @default.
• W2099687964 citedByCount "57" @default.
• W2099687964 countsByYear W20996879642014 @default.
• W2099687964 countsByYear W20996879642015 @default.
• W2099687964 countsByYear W20996879642016 @default.
• W2099687964 countsByYear W20996879642017 @default.
• W2099687964 countsByYear W20996879642018 @default.
• W2099687964 countsByYear W20996879642019 @default.
• W2099687964 countsByYear W20996879642020 @default.
• W2099687964 countsByYear W20996879642021 @default.
• W2099687964 countsByYear W20996879642022 @default.
• W2099687964 countsByYear W20996879642023 @default.
• W2099687964 crossrefType "journal-article" @default.
• W2099687964 hasAuthorship W2099687964A5022494700 @default.
• W2099687964 hasAuthorship W2099687964A5045290531 @default.
• W2099687964 hasAuthorship W2099687964A5051523162 @default.
• W2099687964 hasAuthorship W2099687964A5086656543 @default.
• W2099687964 hasBestOaLocation W20996879641 @default.
• W2099687964 hasConcept C101762097 @default.
• W2099687964 hasConcept C104317684 @default.
• W2099687964 hasConcept C138885662 @default.
• W2099687964 hasConcept C150194340 @default.
• W2099687964 hasConcept C153911025 @default.
• W2099687964 hasConcept C161733203 @default.
• W2099687964 hasConcept C167625842 @default.
• W2099687964 hasConcept C179716448 @default.
• W2099687964 hasConcept C179926584 @default.
• W2099687964 hasConcept C41895202 @default.
• W2099687964 hasConcept C54355233 @default.
• W2099687964 hasConcept C54985914 @default.
• W2099687964 hasConcept C86339819 @default.
• W2099687964 hasConcept C86803240 @default.
• W2099687964 hasConceptScore W2099687964C101762097 @default.
• W2099687964 hasConceptScore W2099687964C104317684 @default.
• W2099687964 hasConceptScore W2099687964C138885662 @default.
• W2099687964 hasConceptScore W2099687964C150194340 @default.
• W2099687964 hasConceptScore W2099687964C153911025 @default.
• W2099687964 hasConceptScore W2099687964C161733203 @default.
• W2099687964 hasConceptScore W2099687964C167625842 @default.
• W2099687964 hasConceptScore W2099687964C179716448 @default.
• W2099687964 hasConceptScore W2099687964C179926584 @default.
• W2099687964 hasConceptScore W2099687964C41895202 @default.
• W2099687964 hasConceptScore W2099687964C54355233 @default.
• W2099687964 hasConceptScore W2099687964C54985914 @default.
• W2099687964 hasConceptScore W2099687964C86339819 @default.
• W2099687964 hasConceptScore W2099687964C86803240 @default.
• W2099687964 hasIssue "8" @default.

• next