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- W2099690878 abstract "Detailed circular dichroism studies have been carried out to monitor thermal as well as denaturant induced unfolding of CYP175A1 from Thermus thermophilus and its mesophilic homologue, CYP101 from Pseudomonas putida. The unfolding midpoint temperatures for tertiary and secondary structures of the substrate-free CYP175A1 were found to be 83.7 °C and 87 °C respectively, while the corresponding midpoint temperatures for substrate-free CYP101 were at 47.7 °C and 51 °C respectively. The apparent Cm value for GdnHCl induced unfolding of secondary structure of CYP175A1 was found to be 2.6 M. The thermodynamic stability curves determined from GdnHCl induced unfolding of the enzymes at different temperatures, showed that CYP175A1 had higher free energy compared to CYP101 at temperature >10 °C. The results clearly established that the high thermostability of CYP175A1 arises predominantly due to higher enthalpy of the thermostable enzyme compared to CYP101. The larger number of salt bridges was proposed to be responsible for higher enthalpy of stabilization of CYP175A1." @default.
- W2099690878 created "2016-06-24" @default.
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- W2099690878 date "2010-05-01" @default.
- W2099690878 modified "2023-09-23" @default.
- W2099690878 title "Thermodynamic basis of the thermostability of CYP175A1 from Thermus thermophilus" @default.
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- W2099690878 doi "https://doi.org/10.1016/j.ijbiomac.2010.01.014" @default.
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