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• W2099691770 abstract "Uropathogenic Escherichia coli strain J96 carries multiple determinants for fimbrial adhesins. The regulatory protein PapB of P fimbriae has previously been implicated in potential coregulatory events. The focB gene of the F1C fimbria determinant is highly homologous to papB; the translated sequences share 81% identity. In this study we investigated the role of PapB and FocB in regulation of the F1C fimbriae. By using gel mobility shift assays, we showed that FocB binds to sequences in both the pap and foc operons in a somewhat different manner than PapB. The results of both in vitro cross-linking and in vivo oligomerization tests indicated that FocB could function in an oligomeric fashion. Furthermore, our results suggest that PapB and FocB can form heterodimers and that these complexes can repress expression of the foc operon. The effect of FocB on expression of type 1 fimbriae was also tested. Taken together, the results that we present expand our knowledge about a regulatory network for different adhesin gene systems in uropathogenic E. coli and suggest a hierarchy for expression of the fimbrial adhesins." @default.
• W2099691770 created "2016-06-24" @default.
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• W2099691770 date "2008-02-01" @default.
• W2099691770 modified "2023-10-15" @default.
• W2099691770 title "Regulatory Interactions among Adhesin Gene Systems of Uropathogenic <i>Escherichia coli</i>" @default.
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• W2099691770 doi "https://doi.org/10.1128/iai.01010-07" @default.
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