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- W2099707406 abstract "The oral cavity supports a complex and finely balanced consortia of microbial species,many of which co-operate within highly structured biofilm communities. Given theimportance of this microbiome in oral disease, considerable scientific effort has beenput into surveying its diversity, determining the nature of interactions between itsmembers, and exploring its determinants. This dissertation addressed each of theseareas; the three principal objectives were (1) to assess microbial diversity in the mouthusing culture-based methods, (2) to use next-generation sequencing technologies toexplore person-to-person and temporal variation in oral microbiome composition, and(3) to use an in vitro model system to analyse variation in the biofilm forming capacityof members of the oral microbiome.Objective 1: Samples of the microbiome were collected from one individual and platedonto a range of different axenic media, incubated under a range of differentconditions. The diversity of isolates obtained was assessed on the basis of classicalphenotypic characteristics and by using partial 16S rDNA sequence comparison. Twelvespecies were identified, all of which were well-recognised members of the oralmicrobiota.Objective 2: Next-generation sequencing was performed on 16S rDNA fragmentsamplified from plaque samples were collected from the oral cavity of three healthyadult human volunteers each month for a period of eight months. A wide diversity ofOTUs was detected in all samples that could be delineated into 13 phyla and 48families. 60 OTUs could be identified at the species level. As expected, general linearmodels revealed statistically significant variation among the OTUs present in differentindividuals and within individuals over time.Objective 3: Fourteen different oral streptococci strains were screened for biofilmformation using the established microtitre plate biofilm assay. The results of this studywere inconsistent but it appeared that most strains best formed biofilms after aboutfour days of incubation, and by day seven, bacteria had died. Optimisation of thistechnique is required.The results of this dissertation add to current knowledge about the diversity anddynamics of the human oral microbiome. This study has also obtained a set of lowpassage isolates of various members of the human microbiome and has begun tooptimise an in vitro biofilm assay. Together, these will provide a useful resource forfuture exploration of the contribution of individual bacterial species to human oralbiofilm infrastructure." @default.
- W2099707406 created "2016-06-24" @default.
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- W2099707406 date "2015-06-29" @default.
- W2099707406 modified "2023-09-27" @default.
- W2099707406 title "Polyphasic characterisation of the human oral microbiome" @default.
- W2099707406 hasPublicationYear "2015" @default.
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