FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2099769969> ?p ?o ?g. }

• W2099769969 endingPage "733" @default.
• W2099769969 startingPage "725" @default.
• W2099769969 abstract "A novel transcriptional regulator responding to glucose was designed with a substrate-binding protein (SBP) as a probe towards intracellular sensing system for glucose in mammalian cells. A chimeric protein of an SBP for glucose (GBP) and a LacI-type regulator, LacI (SLCP(GL) ), was designed, constructed and characterized using Escherichia coli recombinant protein. We report that SLCP(GL) has a glucose-specific binding ability and an operator-sequence specific DNA-binding ability. The loss of its DNA-binding ability in the presence of glucose suggests a role as a transcriptional regulator in vitro. The glucose-dependent gene regulation function of SLCP(GL) in cells was investigated using mammalian cells co-transfected with SLCP(GL) and Lac operator-fused luciferase gene constructs. The luciferase activity of the transfected cells increased with the glucose concentration in the medium, showing that the expression of the luciferase gene is regulated by SLCP(GL) , which can dissociate from DNA in a glucose concentration-dependent manner. Therefore, we demonstrated that SLCP(GL) functions as a glucose-sensitive transcriptional regulator in mammalian cells. These results reveal the possibility of developing an SBP-based regulator as a probe of intracellular sensing and gene regulation system for mammalian cells in response to a desired ligands depending on the SBP ligand specificity." @default.
• W2099769969 created "2016-06-24" @default.
• W2099769969 creator A5012756708 @default.
• W2099769969 creator A5029088690 @default.
• W2099769969 creator A5046634847 @default.
• W2099769969 creator A5050797306 @default.
• W2099769969 date "2010-12-04" @default.
• W2099769969 modified "2023-10-14" @default.
• W2099769969 title "Construction of a novel glucose-sensing molecule based on a substrate-binding protein for intracellular sensing" @default.
• W2099769969 cites W1594096554 @default.
• W2099769969 cites W1857623540 @default.
• W2099769969 cites W1965205049 @default.
• W2099769969 cites W1969195169 @default.
• W2099769969 cites W1969540020 @default.
• W2099769969 cites W1972784441 @default.
• W2099769969 cites W1979871091 @default.
• W2099769969 cites W1980749266 @default.
• W2099769969 cites W1987198676 @default.
• W2099769969 cites W1988251333 @default.
• W2099769969 cites W1996195231 @default.
• W2099769969 cites W1999979145 @default.
• W2099769969 cites W2005553065 @default.
• W2099769969 cites W2007085003 @default.
• W2099769969 cites W2007405901 @default.
• W2099769969 cites W2009217296 @default.
• W2099769969 cites W2011470843 @default.
• W2099769969 cites W2021685870 @default.
• W2099769969 cites W2021985811 @default.
• W2099769969 cites W2022708539 @default.
• W2099769969 cites W2026013887 @default.
• W2099769969 cites W2026209391 @default.
• W2099769969 cites W2028251960 @default.
• W2099769969 cites W2030509920 @default.
• W2099769969 cites W2036593340 @default.
• W2099769969 cites W2052044284 @default.
• W2099769969 cites W2056014433 @default.
• W2099769969 cites W2062147399 @default.
• W2099769969 cites W2078374645 @default.
• W2099769969 cites W2080960105 @default.
• W2099769969 cites W2087989939 @default.
• W2099769969 cites W2096438955 @default.
• W2099769969 cites W2108419658 @default.
• W2099769969 cites W2110083435 @default.
• W2099769969 cites W2131693592 @default.
• W2099769969 cites W2156172387 @default.
• W2099769969 cites W2156440952 @default.
• W2099769969 cites W2161720159 @default.
• W2099769969 cites W2168968107 @default.
• W2099769969 cites W4239748070 @default.
• W2099769969 doi "https://doi.org/10.1002/bit.23006" @default.
• W2099769969 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21404246" @default.
• W2099769969 hasPublicationYear "2010" @default.
• W2099769969 type Work @default.
• W2099769969 sameAs 2099769969 @default.
• W2099769969 citedByCount "11" @default.
• W2099769969 countsByYear W20997699692012 @default.
• W2099769969 countsByYear W20997699692013 @default.
• W2099769969 countsByYear W20997699692014 @default.
• W2099769969 countsByYear W20997699692015 @default.
• W2099769969 countsByYear W20997699692017 @default.
• W2099769969 countsByYear W20997699692018 @default.
• W2099769969 countsByYear W20997699692021 @default.
• W2099769969 crossrefType "journal-article" @default.
• W2099769969 hasAuthorship W2099769969A5012756708 @default.
• W2099769969 hasAuthorship W2099769969A5029088690 @default.
• W2099769969 hasAuthorship W2099769969A5046634847 @default.
• W2099769969 hasAuthorship W2099769969A5050797306 @default.
• W2099769969 hasConcept C104317684 @default.
• W2099769969 hasConcept C111335760 @default.
• W2099769969 hasConcept C116569031 @default.
• W2099769969 hasConcept C150194340 @default.
• W2099769969 hasConcept C153911025 @default.
• W2099769969 hasConcept C170493617 @default.
• W2099769969 hasConcept C185592680 @default.
• W2099769969 hasConcept C27153228 @default.
• W2099769969 hasConcept C40767141 @default.
• W2099769969 hasConcept C54009773 @default.
• W2099769969 hasConcept C552990157 @default.
• W2099769969 hasConcept C55493867 @default.
• W2099769969 hasConcept C6929976 @default.
• W2099769969 hasConcept C79879829 @default.
• W2099769969 hasConcept C86803240 @default.
• W2099769969 hasConcept C95444343 @default.
• W2099769969 hasConceptScore W2099769969C104317684 @default.
• W2099769969 hasConceptScore W2099769969C111335760 @default.
• W2099769969 hasConceptScore W2099769969C116569031 @default.
• W2099769969 hasConceptScore W2099769969C150194340 @default.
• W2099769969 hasConceptScore W2099769969C153911025 @default.
• W2099769969 hasConceptScore W2099769969C170493617 @default.
• W2099769969 hasConceptScore W2099769969C185592680 @default.
• W2099769969 hasConceptScore W2099769969C27153228 @default.
• W2099769969 hasConceptScore W2099769969C40767141 @default.
• W2099769969 hasConceptScore W2099769969C54009773 @default.
• W2099769969 hasConceptScore W2099769969C552990157 @default.
• W2099769969 hasConceptScore W2099769969C55493867 @default.
• W2099769969 hasConceptScore W2099769969C6929976 @default.
• W2099769969 hasConceptScore W2099769969C79879829 @default.
• W2099769969 hasConceptScore W2099769969C86803240 @default.

• next