Matches in SemOpenAlex for { <https://semopenalex.org/work/W2099852313> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2099852313 endingPage "2731" @default.
- W2099852313 startingPage "2723" @default.
- W2099852313 abstract "By chemical and enzymatic methods, two stable complexes between Escherichia coli RNA polymerase and a linear DNA fragment carrying the lac UV5 promoter have been identified. In these binary complexes, DNA can adopt two alternate conformations as a function of temperature. Contacts between RNA polymerase and the DNA phosphate backbone are indistinguishable in these two forms, as revealed by probing with pancreatic DNase I. Protection of enhancement of the reactivity of the bases toward (CH3)2SO4 occurs, however, only in the form that predominates above 22 degrees C, RPo. The form stable at low temperature, RPi, is a closed complex since no single-stranded region is detectable in the DNA. The strong temperature dependence of the equilibrium constant, the midpoint value of the transition, and the rate of conversion between these two forms are in close agreement with a series of measurements performed by using a transcriptional assay and reported in the preceding paper [Buc, H., & McClure, W. R. (1985) Biochemistry (preceding paper in this issue)]. These data further support the postulated mechanism of open complex formation involving three sequential steps: R + P in equilibrium RPc in equilibrium RPi in equilibrium RPo. The binary complex RPc, which accumulates transiently at 37 degrees C before the isomerization leading to open complex formation, is not significantly protected against enzymatic cleavage or chemical modification and is therefore distinct from RPi and RPo.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
- W2099852313 created "2016-06-24" @default.
- W2099852313 creator A5005907388 @default.
- W2099852313 creator A5009031124 @default.
- W2099852313 creator A5073092630 @default.
- W2099852313 date "1985-05-21" @default.
- W2099852313 modified "2023-10-16" @default.
- W2099852313 title "Changes in the DNA structure of the lac UV5 promoter during formation of an open complex with Escherichia coli RNA polymerase" @default.
- W2099852313 cites W1558516755 @default.
- W2099852313 cites W1711115402 @default.
- W2099852313 cites W1963817464 @default.
- W2099852313 cites W1980505207 @default.
- W2099852313 cites W1989080074 @default.
- W2099852313 cites W1996261926 @default.
- W2099852313 cites W1996642589 @default.
- W2099852313 cites W2003636265 @default.
- W2099852313 cites W2019895587 @default.
- W2099852313 cites W2030551247 @default.
- W2099852313 cites W2034739843 @default.
- W2099852313 cites W2057011469 @default.
- W2099852313 cites W2067362564 @default.
- W2099852313 cites W2088351463 @default.
- W2099852313 cites W2094861116 @default.
- W2099852313 cites W2113394490 @default.
- W2099852313 cites W2126758146 @default.
- W2099852313 cites W2146784596 @default.
- W2099852313 cites W248136678 @default.
- W2099852313 cites W260734399 @default.
- W2099852313 cites W2887798820 @default.
- W2099852313 doi "https://doi.org/10.1021/bi00332a019" @default.
- W2099852313 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3896305" @default.
- W2099852313 hasPublicationYear "1985" @default.
- W2099852313 type Work @default.
- W2099852313 sameAs 2099852313 @default.
- W2099852313 citedByCount "123" @default.
- W2099852313 countsByYear W20998523132015 @default.
- W2099852313 countsByYear W20998523132016 @default.
- W2099852313 countsByYear W20998523132017 @default.
- W2099852313 countsByYear W20998523132019 @default.
- W2099852313 countsByYear W20998523132021 @default.
- W2099852313 countsByYear W20998523132022 @default.
- W2099852313 crossrefType "journal-article" @default.
- W2099852313 hasAuthorship W2099852313A5005907388 @default.
- W2099852313 hasAuthorship W2099852313A5009031124 @default.
- W2099852313 hasAuthorship W2099852313A5073092630 @default.
- W2099852313 hasConcept C104317684 @default.
- W2099852313 hasConcept C153911025 @default.
- W2099852313 hasConcept C181199279 @default.
- W2099852313 hasConcept C185592680 @default.
- W2099852313 hasConcept C194232998 @default.
- W2099852313 hasConcept C2776449523 @default.
- W2099852313 hasConcept C547475151 @default.
- W2099852313 hasConcept C552990157 @default.
- W2099852313 hasConcept C55493867 @default.
- W2099852313 hasConcept C67705224 @default.
- W2099852313 hasConcept C71240020 @default.
- W2099852313 hasConcept C82381507 @default.
- W2099852313 hasConcept C86756495 @default.
- W2099852313 hasConcept C86803240 @default.
- W2099852313 hasConceptScore W2099852313C104317684 @default.
- W2099852313 hasConceptScore W2099852313C153911025 @default.
- W2099852313 hasConceptScore W2099852313C181199279 @default.
- W2099852313 hasConceptScore W2099852313C185592680 @default.
- W2099852313 hasConceptScore W2099852313C194232998 @default.
- W2099852313 hasConceptScore W2099852313C2776449523 @default.
- W2099852313 hasConceptScore W2099852313C547475151 @default.
- W2099852313 hasConceptScore W2099852313C552990157 @default.
- W2099852313 hasConceptScore W2099852313C55493867 @default.
- W2099852313 hasConceptScore W2099852313C67705224 @default.
- W2099852313 hasConceptScore W2099852313C71240020 @default.
- W2099852313 hasConceptScore W2099852313C82381507 @default.
- W2099852313 hasConceptScore W2099852313C86756495 @default.
- W2099852313 hasConceptScore W2099852313C86803240 @default.
- W2099852313 hasIssue "11" @default.
- W2099852313 hasLocation W20998523131 @default.
- W2099852313 hasLocation W20998523132 @default.
- W2099852313 hasOpenAccess W2099852313 @default.
- W2099852313 hasPrimaryLocation W20998523131 @default.
- W2099852313 hasRelatedWork W1972665833 @default.
- W2099852313 hasRelatedWork W1976736675 @default.
- W2099852313 hasRelatedWork W2002473487 @default.
- W2099852313 hasRelatedWork W2019087025 @default.
- W2099852313 hasRelatedWork W2023767520 @default.
- W2099852313 hasRelatedWork W2025398173 @default.
- W2099852313 hasRelatedWork W2025468017 @default.
- W2099852313 hasRelatedWork W2037443437 @default.
- W2099852313 hasRelatedWork W2074484440 @default.
- W2099852313 hasRelatedWork W2413421032 @default.
- W2099852313 hasVolume "24" @default.
- W2099852313 isParatext "false" @default.
- W2099852313 isRetracted "false" @default.
- W2099852313 magId "2099852313" @default.
- W2099852313 workType "article" @default.