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- W2099877869 abstract "ABSTRACT Haemophilus ducreyi , the causative agent of chancroid, is highly resistant to the complement-mediated bactericidal activity of normal human serum (NHS). Previously, we identified DsrA (for ducreyi serum resistance A), a major factor required for expression of the serum resistance phenotype in H. ducreyi . We describe here a second outer membrane protein, DltA (for ducreyi lectin A), which also contributes to serum resistance in H. ducreyi . Isogenic dltA mutants, constructed in 35000HP wild-type and FX517 dsrA backgrounds, were more susceptible to the bactericidal effects of NHS than each respective parent, demonstrating the additive effect of the mutations. Furthermore, expression of dltA in H. influenzae strain Rd rendered this highly susceptible strain partially resistant to 5% NHS compared to a vector-control strain. Although primary basic local alignment search tool analysis of the dltA open reading frame revealed no close bacterial homologue, similarity to the β-chain of the eukaryotic lectin ricin was noted. DltA shares highly conserved structural motifs with the ricin β chain, such as cysteines and lectin-binding domains. To determine whether dltA was a lectin, ligand blots and affinity chromatography experiments were performed. DltA was affinity purified on immobilized lactose and N -acetylgalactosamine, and N-glycosylated but not glycosidase-treated model glycoproteins bound DltA. These data indicate that DltA is a lectin with specificity for lactose-related carbohydrates (CHO) and is important for H. ducreyi serum resistance." @default.
- W2099877869 created "2016-06-24" @default.
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- W2099877869 creator A5084145394 @default.
- W2099877869 date "2004-06-01" @default.
- W2099877869 modified "2023-09-23" @default.
- W2099877869 title "A Novel Lectin, DltA, Is Required for Expression of a Full Serum Resistance Phenotype in<i>Haemophilus ducreyi</i>" @default.
- W2099877869 cites W1525730872 @default.
- W2099877869 cites W1558542220 @default.
- W2099877869 cites W1752606869 @default.
- W2099877869 cites W1818347659 @default.
- W2099877869 cites W1830131811 @default.
- W2099877869 cites W1850124138 @default.
- W2099877869 cites W1858949917 @default.
- W2099877869 cites W1864976436 @default.
- W2099877869 cites W1871392693 @default.
- W2099877869 cites W1871516478 @default.
- W2099877869 cites W1921249079 @default.
- W2099877869 cites W1921730143 @default.
- W2099877869 cites W1922098314 @default.
- W2099877869 cites W1943943023 @default.
- W2099877869 cites W1964020649 @default.
- W2099877869 cites W1978194678 @default.
- W2099877869 cites W1981174591 @default.
- W2099877869 cites W1985782125 @default.
- W2099877869 cites W1986018380 @default.
- W2099877869 cites W1993683370 @default.
- W2099877869 cites W2000389746 @default.
- W2099877869 cites W2003521206 @default.
- W2099877869 cites W2016012006 @default.
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- W2099877869 cites W2024747540 @default.
- W2099877869 cites W2027274717 @default.
- W2099877869 cites W2027567211 @default.
- W2099877869 cites W2027908852 @default.
- W2099877869 cites W2035814555 @default.
- W2099877869 cites W2037448992 @default.
- W2099877869 cites W2047798559 @default.
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- W2099877869 cites W2088808709 @default.
- W2099877869 cites W2090273752 @default.
- W2099877869 cites W2090508190 @default.
- W2099877869 cites W2090879318 @default.
- W2099877869 cites W2097191298 @default.
- W2099877869 cites W2100110490 @default.
- W2099877869 cites W2100837269 @default.
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- W2099877869 cites W2115227037 @default.
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- W2099877869 cites W2116980164 @default.
- W2099877869 cites W2117389146 @default.
- W2099877869 cites W2124522718 @default.
- W2099877869 cites W2124694773 @default.
- W2099877869 cites W2125724987 @default.
- W2099877869 cites W2134210196 @default.
- W2099877869 cites W2134840969 @default.
- W2099877869 cites W2141195253 @default.
- W2099877869 cites W2148359654 @default.
- W2099877869 cites W2149379604 @default.
- W2099877869 cites W2149628876 @default.
- W2099877869 cites W2151063485 @default.
- W2099877869 cites W2152200583 @default.
- W2099877869 cites W2152662332 @default.
- W2099877869 cites W2155189175 @default.
- W2099877869 cites W2155259518 @default.
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- W2099877869 cites W2159542619 @default.
- W2099877869 cites W2163407499 @default.
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- W2099877869 cites W2169158441 @default.
- W2099877869 cites W2169743329 @default.
- W2099877869 cites W2172188425 @default.
- W2099877869 cites W2250068495 @default.
- W2099877869 cites W3911104 @default.
- W2099877869 cites W4230785217 @default.
- W2099877869 cites W4300119281 @default.
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- W2099877869 doi "https://doi.org/10.1128/iai.72.6.3418-3428.2004" @default.
- W2099877869 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/415671" @default.
- W2099877869 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15155648" @default.
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