FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2099883909> ?p ?o ?g. }

• W2099883909 endingPage "4557" @default.
• W2099883909 startingPage "4540" @default.
• W2099883909 abstract "This study on human cytochrome P450 reductase (CPR) presents a comprehensive analysis of the thermodynamic and kinetic effects of pH and solvent on two- and four-electron reduction in this diflavin enzyme. pH-dependent redox potentiometry revealed that the thermodynamic equilibrium between various two-electron reduced enzyme species (FMNH*,FADH*; FMN,FADH2; FMNH2,FAD) is independent of pH. No shift from the blue, neutral di-semiquinone (FMNH*,FADH*) towards the red, anionic species is observed upon increasing the pH from 6.5 to 8.5. Spectrophotometric analysis of events following the mixing of oxidized CPR and NADPH (1 to 1) in a stopped-flow instrument demonstrates that the establishment of this thermodynamic equilibrium becomes a very slow process at elevated pH, indicative of a pH-gating mechanism. The final level of blue di-semiquinone formation is found to be pH independent. Stopped-flow experiments using excess NADPH over CPR provide evidence that both pH and solvent significantly influence the kinetic exposure of the blue di-semiquinone intermediate, yet the observed rate constants are essentially pH independent. Thus, the kinetic pH-gating mechanism under stoichiometric conditions is of no significant kinetic relevance for four-electron reduction, but rather modulates the observed semiquinone absorbance at 600 nm in a pH-dependent manner. The use of proton inventory experiments and primary kinetic isotope effects are described as kinetic tools to disentangle the intricate pH-dependent kinetic mechanism in CPR. Our analysis of the pH and isotope dependence in human CPR reveals previously hidden complexity in the mechanism of electron transfer in this complex flavoprotein." @default.
• W2099883909 created "2016-06-24" @default.
• W2099883909 creator A5032255280 @default.
• W2099883909 creator A5058612126 @default.
• W2099883909 creator A5059752371 @default.
• W2099883909 creator A5065173176 @default.
• W2099883909 date "2008-08-04" @default.
• W2099883909 modified "2023-10-16" @default.
• W2099883909 title "Inter-flavin electron transfer in cytochrome P450 reductase - effects of solvent and pH identify hidden complexity in mechanism" @default.
• W2099883909 cites W126145571 @default.
• W2099883909 cites W1485515329 @default.
• W2099883909 cites W1488421511 @default.
• W2099883909 cites W1488511002 @default.
• W2099883909 cites W1554826669 @default.
• W2099883909 cites W1561190910 @default.
• W2099883909 cites W1565796024 @default.
• W2099883909 cites W1577480508 @default.
• W2099883909 cites W1579297624 @default.
• W2099883909 cites W1597669330 @default.
• W2099883909 cites W1603947018 @default.
• W2099883909 cites W1675570769 @default.
• W2099883909 cites W1756287317 @default.
• W2099883909 cites W1828405647 @default.
• W2099883909 cites W1978235062 @default.
• W2099883909 cites W1985939956 @default.
• W2099883909 cites W1986080936 @default.
• W2099883909 cites W1989194230 @default.
• W2099883909 cites W1990741945 @default.
• W2099883909 cites W1993081084 @default.
• W2099883909 cites W2000443708 @default.
• W2099883909 cites W2003672482 @default.
• W2099883909 cites W2004103100 @default.
• W2099883909 cites W2005194798 @default.
• W2099883909 cites W2006352258 @default.
• W2099883909 cites W2006465601 @default.
• W2099883909 cites W2008025632 @default.
• W2099883909 cites W2016281849 @default.
• W2099883909 cites W2016492332 @default.
• W2099883909 cites W2021296090 @default.
• W2099883909 cites W2026098747 @default.
• W2099883909 cites W2031904472 @default.
• W2099883909 cites W2032647645 @default.
• W2099883909 cites W2044740207 @default.
• W2099883909 cites W2047016674 @default.
• W2099883909 cites W2054166508 @default.
• W2099883909 cites W2059328616 @default.
• W2099883909 cites W2062254955 @default.
• W2099883909 cites W2067691482 @default.
• W2099883909 cites W2068906133 @default.
• W2099883909 cites W2069819653 @default.
• W2099883909 cites W2070424612 @default.
• W2099883909 cites W2071961369 @default.
• W2099883909 cites W2072284369 @default.
• W2099883909 cites W2077301367 @default.
• W2099883909 cites W2078331312 @default.
• W2099883909 cites W2079000200 @default.
• W2099883909 cites W2079463072 @default.
• W2099883909 cites W2080167437 @default.
• W2099883909 cites W2082207917 @default.
• W2099883909 cites W2089254396 @default.
• W2099883909 cites W2093344389 @default.
• W2099883909 cites W2094116568 @default.
• W2099883909 cites W2095216354 @default.
• W2099883909 cites W2098096628 @default.
• W2099883909 cites W2129558307 @default.
• W2099883909 cites W2135568463 @default.
• W2099883909 cites W2147814630 @default.
• W2099883909 cites W2157445553 @default.
• W2099883909 cites W2158330515 @default.
• W2099883909 cites W2171437001 @default.
• W2099883909 cites W2200991233 @default.
• W2099883909 cites W4238686153 @default.
• W2099883909 doi "https://doi.org/10.1111/j.1742-4658.2008.06597.x" @default.
• W2099883909 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18681889" @default.
• W2099883909 hasPublicationYear "2008" @default.
• W2099883909 type Work @default.
• W2099883909 sameAs 2099883909 @default.
• W2099883909 citedByCount "39" @default.
• W2099883909 countsByYear W20998839092012 @default.
• W2099883909 countsByYear W20998839092013 @default.
• W2099883909 countsByYear W20998839092014 @default.
• W2099883909 countsByYear W20998839092015 @default.
• W2099883909 countsByYear W20998839092016 @default.
• W2099883909 countsByYear W20998839092017 @default.
• W2099883909 countsByYear W20998839092019 @default.
• W2099883909 countsByYear W20998839092021 @default.
• W2099883909 countsByYear W20998839092023 @default.
• W2099883909 crossrefType "journal-article" @default.
• W2099883909 hasAuthorship W2099883909A5032255280 @default.
• W2099883909 hasAuthorship W2099883909A5058612126 @default.
• W2099883909 hasAuthorship W2099883909A5059752371 @default.
• W2099883909 hasAuthorship W2099883909A5065173176 @default.
• W2099883909 hasBestOaLocation W20998839091 @default.
• W2099883909 hasConcept C121332964 @default.
• W2099883909 hasConcept C123669783 @default.
• W2099883909 hasConcept C135889238 @default.
• W2099883909 hasConcept C178790620 @default.
• W2099883909 hasConcept C179104552 @default.

• next