Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100019242> ?p ?o ?g. }
- W2100019242 endingPage "1884" @default.
- W2100019242 startingPage "1871" @default.
- W2100019242 abstract "The eukaryotic transcript elongation factor TFIIS is encoded by a nonessential gene, PPR2, in Saccharomyces cerevisiae. Disruptions of PPR2 are lethal in conjunction with a disruption in the nonessential gene TAF14/TFG3. While investigating which of the Taf14p-containing complexes may be responsible for the synthetic lethality between ppr2Delta and taf14Delta, we discovered genetic interactions between PPR2 and both TFG1 and TFG2 encoding the two larger subunits of the TFIIF complex that also contains Taf14p. Mutant alleles of tfg1 or tfg2 that render cells cold sensitive have improved growth at low temperature in the absence of TFIIS. Remarkably, the amino-terminal 130 amino acids of TFIIS, which are dispensable for the known in vitro and in vivo activities of TFIIS, are required to complement the lethality in taf14Delta ppr2Delta cells. Analyses of deletion and chimeric gene constructs of PPR2 implicate contributions by different regions of this N-terminal domain. No strong common phenotypes were identified for the ppr2Delta and taf14Delta strains, implying that the proteins are not functionally redundant. Instead, the absence of Taf14p in the cell appears to create a dependence on an undefined function of TFIIS mediated by its N-terminal region. This region of TFIIS is also at least in part responsible for the deleterious effect of TFIIS on tfg1 or tfg2 cold-sensitive cells. Together, these results suggest a physiologically relevant functional connection between TFIIS and TFIIF." @default.
- W2100019242 created "2016-06-24" @default.
- W2100019242 creator A5015185317 @default.
- W2100019242 creator A5022667027 @default.
- W2100019242 creator A5029493897 @default.
- W2100019242 creator A5044011031 @default.
- W2100019242 creator A5046168818 @default.
- W2100019242 creator A5046732964 @default.
- W2100019242 creator A5067826029 @default.
- W2100019242 creator A5072640420 @default.
- W2100019242 date "2006-08-01" @default.
- W2100019242 modified "2023-10-14" @default.
- W2100019242 title "Genetic Interactions Between TFIIF and TFIIS" @default.
- W2100019242 cites W1480264538 @default.
- W2100019242 cites W1485158076 @default.
- W2100019242 cites W1501611496 @default.
- W2100019242 cites W1505354077 @default.
- W2100019242 cites W1518174569 @default.
- W2100019242 cites W1524374453 @default.
- W2100019242 cites W1547269929 @default.
- W2100019242 cites W1563789995 @default.
- W2100019242 cites W1593921822 @default.
- W2100019242 cites W1606017721 @default.
- W2100019242 cites W1848777874 @default.
- W2100019242 cites W1850569493 @default.
- W2100019242 cites W1856778266 @default.
- W2100019242 cites W1925058512 @default.
- W2100019242 cites W1939319816 @default.
- W2100019242 cites W1964719548 @default.
- W2100019242 cites W1967615956 @default.
- W2100019242 cites W1968917541 @default.
- W2100019242 cites W1973862916 @default.
- W2100019242 cites W1975232273 @default.
- W2100019242 cites W1978989417 @default.
- W2100019242 cites W1979470231 @default.
- W2100019242 cites W1980888037 @default.
- W2100019242 cites W1984786490 @default.
- W2100019242 cites W1986184988 @default.
- W2100019242 cites W1987086781 @default.
- W2100019242 cites W1996183389 @default.
- W2100019242 cites W2002085309 @default.
- W2100019242 cites W2002957811 @default.
- W2100019242 cites W2004617021 @default.
- W2100019242 cites W2004622897 @default.
- W2100019242 cites W2004828705 @default.
- W2100019242 cites W2016533945 @default.
- W2100019242 cites W2026574531 @default.
- W2100019242 cites W2029596636 @default.
- W2100019242 cites W2030465328 @default.
- W2100019242 cites W2031964894 @default.
- W2100019242 cites W2036970927 @default.
- W2100019242 cites W2040327353 @default.
- W2100019242 cites W2044793296 @default.
- W2100019242 cites W2062630554 @default.
- W2100019242 cites W2063000913 @default.
- W2100019242 cites W2074405508 @default.
- W2100019242 cites W2079393473 @default.
- W2100019242 cites W2082908076 @default.
- W2100019242 cites W2084622414 @default.
- W2100019242 cites W2088997237 @default.
- W2100019242 cites W2090392380 @default.
- W2100019242 cites W2090690907 @default.
- W2100019242 cites W2091168900 @default.
- W2100019242 cites W2092238955 @default.
- W2100019242 cites W2094015812 @default.
- W2100019242 cites W2094149905 @default.
- W2100019242 cites W2096275987 @default.
- W2100019242 cites W2100132835 @default.
- W2100019242 cites W2103995020 @default.
- W2100019242 cites W2106317558 @default.
- W2100019242 cites W2108053853 @default.
- W2100019242 cites W2108707557 @default.
- W2100019242 cites W2115476283 @default.
- W2100019242 cites W2119741754 @default.
- W2100019242 cites W2127533921 @default.
- W2100019242 cites W2130784145 @default.
- W2100019242 cites W2133634303 @default.
- W2100019242 cites W2133703753 @default.
- W2100019242 cites W2135068917 @default.
- W2100019242 cites W2136217363 @default.
- W2100019242 cites W2146281325 @default.
- W2100019242 cites W2151221308 @default.
- W2100019242 cites W2158364267 @default.
- W2100019242 cites W2165289894 @default.
- W2100019242 cites W2169333620 @default.
- W2100019242 cites W2170565831 @default.
- W2100019242 cites W2267558770 @default.
- W2100019242 cites W2801944549 @default.
- W2100019242 cites W4206289657 @default.
- W2100019242 cites W4254536048 @default.
- W2100019242 doi "https://doi.org/10.1534/genetics.106.058834" @default.
- W2100019242 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1569716" @default.
- W2100019242 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16648643" @default.
- W2100019242 hasPublicationYear "2006" @default.
- W2100019242 type Work @default.
- W2100019242 sameAs 2100019242 @default.
- W2100019242 citedByCount "10" @default.
- W2100019242 countsByYear W21000192422015 @default.