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• W2100020152 abstract "ASH1 mRNA localizes at the bud tip of late-anaphase yeast, resulting in accumulation of Ash1p in the daughter nucleus. We show that disruption of the secondary structure, but not the protein coding, of all four ASH1 localization elements resulted in RNA and protein delocalization. Localization of both was incrementally restored by replacement of each of the four elements. However, transposition of the elements to the 3'UTR reinstated the RNA, but not the protein, localization. Interestingly, the mutant ASH1 mRNA was translated more efficiently, suggesting that asymmetry of Ash1p resulted from translational inhibition by the localization elements. In support of this, Ash1p asymmetry could be rescued by slowing its translation." @default.
• W2100020152 created "2016-06-24" @default.
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• W2100020152 date "2002-12-01" @default.
• W2100020152 modified "2023-10-12" @default.
• W2100020152 title "Asymmetric Sorting of Ash1p in Yeast Results from Inhibition of Translation by Localization Elements in the mRNA" @default.
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• W2100020152 doi "https://doi.org/10.1016/s1097-2765(02)00694-9" @default.
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