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- W2100031579 abstract "The effect of succinate on the production of cloned glucoamylase by a recombinant yeast system was investigated. Saccharomyces cerevisiae was transformed with the 2 μ-based plasmid YEpSUCSTA containing the SUC2 promoter, the STA signal sequence, and the STA structural gene. Specific glucoamylase expression was 89% higher in a semi-synthetic medium containing succinate than in medium without succinate. This increase in glucoamylase activity in succinate-containing media reflects two roles of succinate; protection of rapid decrease in pH in the culture broth and enhancement of glucoamylase biosynthesis." @default.
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- W2100031579 date "1998-03-01" @default.
- W2100031579 modified "2023-09-27" @default.
- W2100031579 title "Enhancement, by succinate addition, of the production of cloned glucoamylase from recombinant yeast using a SUC2 promoter" @default.
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- W2100031579 doi "https://doi.org/10.1016/s0032-9592(97)00042-3" @default.
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